Tpx2 TPX2, microtubule-associated [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tpx2provided by MGI
Official Full Name: TPX2, microtubule-associatedprovided by MGI
Primary source: MGI:MGI:1919369
See related: Ensembl:ENSMUSG00000027469 AllianceGenome:MGI:1919369
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DIL2; p100; REPP86; 2610005B21Rik
Summary: Enables importin-alpha family protein binding activity and protein kinase binding activity. Involved in mitotic spindle assembly and regulation of mitotic spindle organization. Located in axon hillock and spindle. Is expressed in cerebral cortex ventricular layer; large intestine; nephrogenic zone; and small intestine. Orthologous to human TPX2 (TPX2 microtubule nucleation factor). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in CNS E11.5 (RPKM 33.6), liver E14 (RPKM 29.8) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H1; 2 75.41 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (152689859..152737241)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (152847964..152895321)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Downregulation of TPX2 impairs the antitumor activity of CD8+ T cells in hepatocellular carcinoma.
Title: Downregulation of TPX2 impairs the antitumor activity of CD8+ T cells in hepatocellular carcinoma.
Depleting TPX2 decreases microtubule formation frequency specifically at the tip and the base of the neurite, and these correlate precisely with the regions where active GTP-bound Ran proteins are enriched.
Title: Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons.
Bcl2l10, Tpx2, and Aurka co-localized on the meiotic spindles, and Bcl2l10 was present in the same complex with Tpx2.
Title: Bcl2l10, a new Tpx2 binding partner, is a master regulator of Aurora kinase A in mouse oocytes.
Study demonstrates that the Golgi resident protein GM130 activates the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and further captures them to couple mitotic membranes to the spindle.
Title: GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2 and Capturing Microtubules.
The findings indicated that Tpx2 is essential for maintaining genomic stability through its role in spindle regulation.
Title: Tpx2 controls spindle integrity, genome stability, and tumor development.
Tpx2 redistributes from the nucleus to the apical process, and promotes nuclear migration during G2-phase by altering microtubule organization.
Title: Regulation of interkinetic nuclear migration by cell cycle-coupled active and passive mechanisms in the developing brain.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D2Mit285 (e-PCR), detects polymorphism
- UniSTS:116279

NoName (e-PCR)
- UniSTS:234560

D2Dcr107 (e-PCR), detects polymorphism
- UniSTS:507585

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables importin-alpha family protein binding	IDA Inferred from Direct Assay more info	PubMed
enables importin-alpha family protein binding	ISO Inferred from Sequence Orthology more info
enables importin-alpha family protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables molecular adaptor activity	IEA Inferred from Electronic Annotation more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in activation of protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in activation of protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in microtubule nucleation	IEA Inferred from Electronic Annotation more info
involved_in microtubule nucleation	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of microtubule depolymerization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of microtubule depolymerization	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spindle assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in aster	ISO Inferred from Sequence Orthology more info
located_in axon hillock	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle	ISO Inferred from Sequence Orthology more info
located_in spindle pole	IEA Inferred from Electronic Annotation more info
colocalizes_with spindle pole	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: targeting protein for Xklp2

Names: TPX2, microtubule-associated protein homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001141975.2 → NP_001135447.1 targeting protein for Xklp2

See identical proteins and their annotated locations for NP_001135447.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1.

Source sequence(s)

AL731857, AL833801

Consensus CDS

CCDS16900.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Related

ENSMUSP00000136457.2, ENSMUST00000178997.8

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein
NM_001141976.2 → NP_001135448.1 targeting protein for Xklp2

See identical proteins and their annotated locations for NP_001135448.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1.

Source sequence(s)

AL731857, AL833801

Consensus CDS

CCDS16900.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein
NM_001141977.2 → NP_001135449.1 targeting protein for Xklp2

See identical proteins and their annotated locations for NP_001135449.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript.

Source sequence(s)

AL731857, AL833801

Consensus CDS

CCDS16900.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Related

ENSMUSP00000128888.2, ENSMUST00000164120.8

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein
NM_001141978.2 → NP_001135450.1 targeting protein for Xklp2

See identical proteins and their annotated locations for NP_001135450.1

Status: VALIDATED

Description

Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1.

Source sequence(s)

AL731857, AL833801

Consensus CDS

CCDS16900.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Related

ENSMUSP00000105441.2, ENSMUST00000109816.8

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein
NM_028109.5 → NP_082385.3 targeting protein for Xklp2

See identical proteins and their annotated locations for NP_082385.3

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1.

Source sequence(s)

AL731857, AL833801

Consensus CDS

CCDS16900.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Related

ENSMUSP00000028969.9, ENSMUST00000028969.9

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

152689859..152737241

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006500213.3 → XP_006500276.1 targeting protein for Xklp2 isoform X1

See identical proteins and their annotated locations for XP_006500276.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein
XM_006500215.3 → XP_006500278.1 targeting protein for Xklp2 isoform X1

See identical proteins and their annotated locations for XP_006500278.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein
XM_006500214.3 → XP_006500277.1 targeting protein for Xklp2 isoform X1

See identical proteins and their annotated locations for XP_006500277.1

UniProtKB/Swiss-Prot

A2APB8, Q3U500, Q6P9S6

UniProtKB/TrEMBL

Q8BTJ3

Conserved Domains (3) summary

pfam06886
Location:661 → 717

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 68

Aurora-A_bind; Aurora-A binding

pfam12214
Location:367 → 489

TPX2_importin; Cell cycle regulated microtubule associated protein