U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from Protein

    • Showing Current items.

    ABA3 molybdenum cofactor sulfurase (LOS5) (ABA3) [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 838224, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    ABA3
    Official Full Name
    molybdenum cofactor sulfurase (LOS5) (ABA3)
    Primary source
    TAIR:AT1G16540
    Locus tag
    AT1G16540
    See related
    Araport:AT1G16540
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ABA DEFICIENT 3; ACI2; ALTERED CHLOROPLAST IMPORT 2; ATABA3; AtLOS5; F19K19.13; F19K19_13; LOS5; LOW OSMOTIC STRESS 5; MOLYBDENUM COFACTOR SULFURASE; SIR3; SIRTINOL RESISTANT 3
    Summary
    Encodes molybdenum cofactor sulfurase. Involved in Moco biosynthesis. Involved in the conversion of ABA-aldehyde to ABA, the last step of abscisic acid (ABA) biosynthesis. <i>sir</i> loss-of-function mutants are resistant to sirtinol, a modulator of auxin signaling.N terminal domain is similar to bacterial NifS suggesting a common mechanism for sulphur mobilization and transfer. Also involved in protein import into chloroplasts.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See ABA3 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    20
    Sequence:
    Chromosome: 1; NC_003070.9 (5659370..5665488)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene interactor of constitutive active ROPs protein Neighboring gene ASYMMETRIC LEAVES 2-like 9 Neighboring gene miscRNA Neighboring gene Per1-like family protein Neighboring gene ncRNA

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Increased abscisic acid levels in transgenic maize overexpressing AtLOS5 mediated root ion fluxes and leaf water status under salt stress. Zhang J, et al. J Exp Bot, 2016 Mar. PMID 26743432, Free PMC Article
    2. Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci. Léon-Kloosterziel KM, et al. Plant J, 1996 Oct. PMID 8893542
    3. The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress- and osmotic stress-responsive gene expression. Xiong L, et al. Plant Cell, 2001 Sep. PMID 11549764, Free PMC Article
    4. Characterization of the NifS-like domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration. Heidenreich T, et al. J Biol Chem, 2005 Feb 11. PMID 15561708
    5. Binding of sulfurated molybdenum cofactor to the C-terminal domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration. Wollers S, et al. J Biol Chem, 2008 Apr 11. PMID 18258600

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18.
      Title: The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18.
    2. AtLOS5 overexpression induced the expression of ABA biosynthetic genes to promote abscissic acid accumulation.
      Title: Increased abscisic acid levels in transgenic maize overexpressing AtLOS5 mediated root ion fluxes and leaf water status under salt stress.
    3. These data suggest that overexpression of LOS5/ABA3 could improve drought tolerance in transgenic soybean via enhanced abscissic acid accumulation.
      Title: Expression of an Arabidopsis molybdenum cofactor sulphurase gene in soybean enhances drought tolerance and increases yield under field conditions.
    4. Data indicate that overexpression of Arabidopsis molybdenum cofactor sulfurase gene (LOS5) in maize markedly enhanced the expression of ZmAO and aldehyde oxidase (AO) activity, leading to abscisic acid (ABA) accumulation and increased drought tolerance.
      Title: Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize (Zea mays L.).
    5. Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance.
      Title: Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton.
    6. Identification of persulfide-binding and disulfide-forming cysteine residues in the NifS-like domain of the molybdenum cofactor sulfurase ABA3.
      Title: Identification of persulfide-binding and disulfide-forming cysteine residues in the NifS-like domain of the molybdenum cofactor sulfurase ABA3 by cysteine-scanning mutagenesis.
    7. Overexpression of LOS5 in transgenic tobacco can enhance drought tolerance.
      Title: Arabidopsis LOS5/ABA3 overexpression in transgenic tobacco (Nicotiana tabacum cv. Xanthi-nc) results in enhanced drought tolerance.
    8. Mutation in Moco-sulfurase causes diminished aldehyde oxidase and xanthine dehydrogenase activities and loss of efficient chloroplast protein import.
      Title: A forward genetic screen to explore chloroplast protein import in vivo identifies Moco sulfurase, pivotal for ABA and IAA biosynthesis and purine turnover.
    9. the C-terminal domain of ABA3 might act as a scaffold protein where prebound desulfo-molybdenum cofactor is converted into sulfurated cofactor prior to activation of aldehyde oxidase and xanthine dehydrogenase.
      Title: Binding of sulfurated molybdenum cofactor to the C-terminal domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration.
    10. analysis of the NifS-like domain of ABA3 from Arabidopsis thaliana which has a role in molybdenum cofactor sulfuration
      Title: Characterization of the NifS-like domain of ABA3 from Arabidopsis thaliana provides insight into the mechanism of molybdenum cofactor sulfuration.
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    General gene information

    Go to the top of the page Help
    NM_001332231.1
    NM_101519.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables molybdenum cofactor sulfurtransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables molybdenum cofactor sulfurtransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables selenocysteine lyase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within abscisic acid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within auxin-activated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within defense response to bacterium IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within molybdenum incorporation into molybdenum-molybdopterin complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within protein import into chloroplast stroma IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to glucose IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to heat IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to osmotic stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to salt stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within stomatal movement IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within sugar mediated signaling pathway TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cellular_component ND
    No biological Data available
    more info
    		  
    located_in cytoplasm ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    molybdenum cofactor sulfurase (LOS5) (ABA3)
    NP_564001.1
    • ABA DEFICIENT 3 (ABA3); CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), MOSC, N-terminal beta barrel (InterPro:IPR005303), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Molybdenum cofactor sulfurase, C-terminal (InterPro:IPR005302), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: Molybdenum cofactor sulfurase family protein (TAIR:AT1G30910.1); Has 7940 Blast hits to 7904 proteins in 1914 species: Archae - 94; Bacteria - 5396; Metazoa - 390; Fungi - 394; Plants - 292; Viruses - 1; Other Eukaryotes - 1373 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      5659370..5665488
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001332230.1 → NP_001322901.1  molybdenum cofactor sulfurase (LOS5) (ABA3) [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178WK01, A0A1P8AVE2, A0A5S9USV8

    2. NM_101519.3 → NP_564001.1  molybdenum cofactor sulfurase (LOS5) (ABA3) [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_564001.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9C5X8, Q9FX72
      UniProtKB/TrEMBL
      A0A178WK01, A0A5S9USV8
      Conserved Domains (4) summary
      PLN02724
      Location:1 → 819
      PLN02724; Molybdenum cofactor sulfurase
      pfam03473
      Location:680 → 814
      MOSC; MOSC domain
      pfam03476
      Location:530 → 649
      MOSC_N; MOSC N-terminal beta barrel domain
      cl18945
      Location:33 → 489
      AAT_I; Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the ...

    3. NM_001332231.1 → NP_001322902.1  molybdenum cofactor sulfurase (LOS5) (ABA3) [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178WIB1, A0A1P8AVG9
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9C5X8.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.