Smurf1 SMAD specific E3 ubiquitin protein ligase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Smurf1provided by MGI
Official Full Name: SMAD specific E3 ubiquitin protein ligase 1provided by MGI
Primary source: MGI:MGI:1923038
See related: Ensembl:ENSMUSG00000038780 AllianceGenome:MGI:1923038
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA1625; 4930431E10Rik
Summary: Predicted to enable SMAD binding activity; signaling receptor binding activity; and ubiquitin protein ligase activity. Involved in type 2 mitophagy. Acts upstream of or within negative regulation of ossification and protein ubiquitination. Predicted to be located in axon and neuronal cell body. Predicted to be active in cytoplasm and plasma membrane. Predicted to colocalize with mitochondrion. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system; and respiratory system. Orthologous to human SMURF1 (SMAD specific E3 ubiquitin protein ligase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in whole brain E14.5 (RPKM 16.3), ovary adult (RPKM 16.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 84.83 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (144813305..144902657, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (144876495..144965899, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SMURF1 activates the cGAS/STING/IFN-1 signal axis by mediating YY1 ubiquitination to accelerate the progression of lupus nephritis.
Title: SMURF1 activates the cGAS/STING/IFN-1 signal axis by mediating YY1 ubiquitination to accelerate the progression of lupus nephritis.
Smurf1 Modulates Smad Signaling Pathway in Fibrotic Cataract Formation.
Title: Smurf1 Modulates Smad Signaling Pathway in Fibrotic Cataract Formation.
piR-368 promotes odontoblastic differentiation of dental papilla cells via the Smad1/5 signaling pathway by targeting Smurf1.
Title: piR-368 promotes odontoblastic differentiation of dental papilla cells via the Smad1/5 signaling pathway by targeting Smurf1.
Talin-1 inhibits Smurf1-mediated Stat3 degradation to modulate beta-cell proliferation and mass in mice.
Title: Talin-1 inhibits Smurf1-mediated Stat3 degradation to modulate β-cell proliferation and mass in mice.
A Golgi-resident GPR108 cooperates with E3 ubiquitin ligase Smurf1 to suppress antiviral innate immunity.
Title: A Golgi-resident GPR108 cooperates with E3 ubiquitin ligase Smurf1 to suppress antiviral innate immunity.
The degradation of TGR5 mediated by Smurf1 contributes to diabetic nephropathy.
Title: The degradation of TGR5 mediated by Smurf1 contributes to diabetic nephropathy.
Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA.
Title: Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA.
HSP90beta chaperoning SMURF1-mediated LATS proteasomal degradation in the regulation of bone formation.
Title: HSP90β chaperoning SMURF1-mediated LATS proteasomal degradation in the regulation of bone formation.
Deletion of Smurf1 attenuates liver steatosis via stabilization of p53.
Title: Deletion of Smurf1 attenuates liver steatosis via stabilization of p53.
MicroRNA 379 Regulates Klotho Deficiency-Induced Cardiomyocyte Apoptosis Via Repression of Smurf1.
Title: MicroRNA 379 Regulates Klotho Deficiency-Induced Cardiomyocyte Apoptosis Via Repression of Smurf1.

Submit: New GeneRIF Correction See all GeneRIFs (44)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB445477 (e-PCR)
- UniSTS:207913

Smurf1 (e-PCR), detects polymorphism
- UniSTS:497286

Smurf1 (e-PCR), detects polymorphism
- UniSTS:527557

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables I-SMAD binding	ISO Inferred from Sequence Orthology more info
enables R-SMAD binding	ISO Inferred from Sequence Orthology more info
enables SMAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables activin receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transforming growth factor beta receptor binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	TAS Traceable Author Statement more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in BMP signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in engulfment of target by autophagosome	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of BMP signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of activin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of ossification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendrite extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein export from nucleus	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein targeting to vacuole involved in autophagy	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor catabolic process	ISO Inferred from Sequence Orthology more info
involved_in substrate localization to autophagosome	ISO Inferred from Sequence Orthology more info
involved_in type 2 mitophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Component	Evidence Code	Pubs
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	TAS Traceable Author Statement more info
colocalizes_with mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase SMURF1

Names: HECT-type E3 ubiquitin transferase SMURF1; Smad ubiquitination regulatory factor 1

NP_001033716.1

EC 2.3.2.26

NP_083714.3

EC 2.3.2.26

XP_006504947.1

EC 2.3.2.26

XP_011239318.1

EC 2.3.2.26

XP_011239319.1

EC 2.3.2.26

XP_036021480.1

EC 2.3.2.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001038627.1 → NP_001033716.1 E3 ubiquitin-protein ligase SMURF1 isoform 1

See identical proteins and their annotated locations for NP_001033716.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer protein (isoform 1).

Source sequence(s)

AC113295, AK015264, AK034736, CB524571, CX568994

Consensus CDS

CCDS39380.1

UniProtKB/Swiss-Prot

Q3U412, Q8K300, Q9CUN6

Related

ENSMUSP00000082827.5, ENSMUST00000085684.11

Conserved Domains (4) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:281 → 313

WW; Domain with 2 conserved Trp (W) residues

smart00119
Location:397 → 728

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd00078
Location:373 → 728

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
NM_029438.3 → NP_083714.3 E3 ubiquitin-protein ligase SMURF1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).

Source sequence(s)

AC113295, AK015264, CB524571, CX568994

Consensus CDS

CCDS39381.1

UniProtKB/TrEMBL

E9Q4K9

Related

ENSMUSP00000098029.3, ENSMUST00000100461.7

Conserved Domains (4) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:281 → 313

WW; Domain with 2 conserved Trp (W) residues

smart00119
Location:397 → 725

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd00078
Location:373 → 725

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

144813305..144902657 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036165587.1 → XP_036021480.1 E3 ubiquitin-protein ligase SMURF1 isoform X3

Conserved Domains (3) summary

cd08382
Location:14 → 128

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:285 → 317

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:377 → 732

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_011241017.3 → XP_011239319.1 E3 ubiquitin-protein ligase SMURF1 isoform X4

Conserved Domains (3) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:236 → 267

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:324 → 679

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_011241016.3 → XP_011239318.1 E3 ubiquitin-protein ligase SMURF1 isoform X2

Conserved Domains (3) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:307 → 339

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:399 → 751

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_006504884.3 → XP_006504947.1 E3 ubiquitin-protein ligase SMURF1 isoform X1

UniProtKB/TrEMBL

E9PYU8

Related

ENSMUSP00000106305.2, ENSMUST00000110677.8

Conserved Domains (3) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:307 → 339

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:399 → 754

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...