Rhot2 ras homolog family member T2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rhot2provided by MGI
Official Full Name: ras homolog family member T2provided by MGI
Primary source: MGI:MGI:2384892
See related: Ensembl:ENSMUSG00000025733 AllianceGenome:MGI:2384892
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Arht2; Miro2
Summary: Predicted to enable GTP binding activity and GTPase activity. Predicted to be involved in cellular homeostasis; mitochondrial outer membrane permeabilization; and mitochondrion transport along microtubule. Located in mitochondrial inner membrane. Is expressed in brain; head; retina inner nuclear layer; and retina outer nuclear layer. Orthologous to human RHOT2 (ras homolog family member T2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in adrenal adult (RPKM 113.1), colon adult (RPKM 76.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 12.93 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (26057431..26063499, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (25838457..25844525, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mitochondrial Miro2 expression levels regulate inter-mitochondrial communication along microtubules in adult cardiomyocytes. Degradation of Miro2 through Parkin-mediated ubiquitination contributes to impaired inter-mitochondrial communication and cardiac dysfunction during hypertrophic heart diseases.
Title: Miro2 Regulates Inter-Mitochondrial Communication in the Heart and Protects Against TAC-Induced Cardiac Dysfunction.
Using knockout mouse embryonic fibroblasts, data show that mitochondrial Rho (Miro) GTPases Miro1 and Miro2 are required for normal mitochondrial cristae architecture and endoplasmic reticulum-mitochondria contacts Sites (ERMCS).
Title: Miro clusters regulate ER-mitochondria contact sites and link cristae organization to the mitochondrial transport machinery.
we show for the first time that inhibition of NOS3 generation in glial cells prevents axonal mitochondrial fission and restores mitochondrial motility to confer protection to axons by preserving Miro-2 levels
Title: NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels.
Due to the multifunctionality of CENP-F, the cellular phenotypes observed upon its depletion are difficult to interpret and there is a need to genetically separate its different functions by preventing binding to selected partners. Here we engineer a CENP-F point-mutant that is deficient in Miro1/2 binding and thus is unable to localize to mitochondria, but retains other localizations.
Title: Miro-dependent mitochondrial pool of CENP-F and its farnesylated C-terminal domain are dispensable for normal development in mice.
both Miro proteins can act to coordinate microtubule-dependent and actin-dependent mitochondrial trafficking, Miro1 preferentially acts to control microtubule-dependent trafficking through kinesin and dynein, while Miro2 plays a more prominent role in coordinating mitochondrial interactions with the actin cytoskeleton through a more efficient recruitment and stability of endogenous Myo19 in the mitochondrial membrane.
Title: Miro proteins coordinate microtubule- and actin-dependent mitochondrial transport and distribution.
Using mouse knockout strategies, we demonstrate that Miro1, as opposed to Miro2, is the primary regulator of mitochondrial transport in both axons and dendrites. Miro1 deletion leads to depletion of mitochondria from distal dendrites but not axons, accompanied by a marked reduction in dendritic complexity. Disrupting postnatal mitochondrial distribution in vivo by deleting Miro1 in mature neurons causes a progressive loss
Title: Loss of Dendritic Complexity Precedes Neurodegeneration in a Mouse Model with Disrupted Mitochondrial Distribution in Mature Dendrites.
Armcx genes regulate mitochondrial trafficking in neurons and interact with Miro 1/2 and Trak2.
Title: The Eutherian Armcx genes regulate mitochondrial trafficking in neurons and interact with Miro and Trak2.
molecular cloning and characterization of a novel mouse cDNA encoding a putative atypical GTPase protein, Arht2
Title: Cloning and characterization of the mouse Arht2 gene which encodes a putative atypical GTPase.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D17Aus57 (e-PCR), detects polymorphism
- UniSTS:142108

AI551153 (e-PCR)
- UniSTS:166039

D17Jcs48 (e-PCR), detects polymorphism
- UniSTS:259289

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular homeostasis	ISO Inferred from Sequence Orthology more info
involved_in cellular homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitochondrial outer membrane permeabilization	ISO Inferred from Sequence Orthology more info
involved_in mitochondrial outer membrane permeabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitochondrion organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrion transport along microtubule	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrion transport along microtubule	ISO Inferred from Sequence Orthology more info
involved_in mitochondrion transport along microtubule	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in mitochondrial inner membrane	HDA more info	PubMed
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: mitochondrial Rho GTPase 2

Names: mitochondrial Rho 2; ras homolog gene family, member T2

NP_001351879.1

EC 3.6.5.-

NP_666111.1

EC 3.6.5.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001364950.1 → NP_001351879.1 mitochondrial Rho GTPase 2 isoform 2

Status: VALIDATED

Source sequence(s)

AC159277

Conserved Domains (5) summary

cd01892
Location:412 → 589

Miro2; Mitochondrial Rho family 2 (Miro2), C-terminal

cd01893
Location:11 → 167

Miro1; Mitochondrial Rho family 1 (Miro1), N-terminal

pfam08355
Location:339 → 406

EF_assoc_1; EF hand associated

pfam08356
Location:218 → 303

EF_assoc_2; EF hand associated

pfam13499
Location:307 → 336

EF-hand_7; EF-hand domain pair
NM_145999.3 → NP_666111.1 mitochondrial Rho GTPase 2 isoform 1

See identical proteins and their annotated locations for NP_666111.1

Status: VALIDATED

Source sequence(s)

AC159277, AY311390

Consensus CDS

CCDS37505.1

UniProtKB/Swiss-Prot

Q8JZN7

Related

ENSMUSP00000044639.10, ENSMUST00000043897.16

Conserved Domains (5) summary

smart00174
Location:7 → 169

RHO; Rho (Ras homology) subfamily of Ras-like small GTPases

cd01892
Location:414 → 591

Miro2; Mitochondrial Rho family 2 (Miro2), C-terminal

cd01893
Location:3 → 169

Miro1; Mitochondrial Rho family 1 (Miro1), N-terminal

pfam08355
Location:341 → 408

EF_assoc_1; EF hand associated

pfam08356
Location:221 → 301

EF_assoc_2; EF hand associated