Nos Nitric oxide synthase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Nosprovided by FlyBase
Official Full Name: Nitric oxide synthaseprovided by FlyBase
Primary source: FLYBASE:FBgn0011676
Locus tag: Dmel_CG6713
See related: AllianceGenome:FB:FBgn0011676
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG6713; Dmel\CG6713; dNos; dNOS; DNOS; DNOS1; dNOS4; drNOSoxy; dsNOS; iNos; iNOS; nNOS; nos; NOS; NOS1; NOS2
Summary: Enables nitric-oxide synthase activity. Involved in several processes, including nitric oxide biosynthetic process; regulation of heart rate; and response to Gram-negative bacterium. Predicted to be located in peroxisome. Predicted to be active in cytosol; nucleus; and plasma membrane. Is expressed in several structures, including CNS glial cell; adult head; ganglia; imaginal disc; and synaptic neuropil domain. Used to study epilepsy and lissencephaly. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); dementia (multiple); eye disease (multiple); reproductive organ cancer (multiple); and vasculitis (multiple). Orthologous to several human genes including NOS1 (nitric oxide synthase 1) and NOS3 (nitric oxide synthase 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 32B1-32B1; 2-43 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (10804274..10837511, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (10804274..10837511, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nitric oxide mediates neuro-glial interaction that shapes Drosophila circadian behavior.
Title: Nitric oxide mediates neuro-glial interaction that shapes Drosophila circadian behavior.
Here we demonstrate that the Drosophila relaxin receptor homolog Lgr3, a leucine-rich repeat-containing G-protein-coupled receptor, is required for Dilp8-dependent growth coordination and developmental delay during the regeneration checkpoint; we demonstrate that Lgr3 activity in both the CNS and PG is necessary for NOS activation in the PG following damage
Title: Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration Is Mediated by Signaling Through the Relaxin Receptor Lgr3 in the Prothoracic Gland.
Nitric Oxide Synthase Regulates Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration
Title: Nitric Oxide Synthase Regulates Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration.
Hb9 governs neuronal specification and differentiation and activates expression of Nos and fd59a in the Drosophila CNS
Title: Genome-wide identification of Drosophila Hb9 targets reveals a pivotal role in directing the transcriptome within eight neuronal lineages, including activation of nitric oxide synthase and Fd59a/Fox-D.
Nitric oxide synthase is not essential for Drosophila development.
Title: Nitric oxide synthase is not essential for Drosophila development.
The suppressed enzyme expression was shown to affect the cell polarity and movement as well as the structural pattern of organs during Drosophila development.
Title: [Nitric oxide synthase mediates regulation of cell polarity and movement during Drosophila melanogaster morphogenesis].
Reductase domain of Drosophila melanogaster Nos: redox transformations, regulation, and similarity to mamalian homologues.
Title: Reductase domain of Drosophila melanogaster nitric-oxide synthase: redox transformations, regulation, and similarity to mammalian homologues.
Oxygenase domain of Drosophila melanogaster Nos: unique kinetic parameters enable a more efficient NO release.
Title: Oxygenase domain of Drosophila melanogaster nitric oxide synthase: unique kinetic parameters enable a more efficient NO release.
biochemical characterization of Drosophila nitric oxide synthase
Title: Characterization of Drosophila nitric oxide synthase: a biochemical study.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables FMN binding	IBA Inferred from Biological aspect of Ancestor more info
enables FMN binding	IEA Inferred from Electronic Annotation more info
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	NAS Non-traceable Author Statement more info	PubMed
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables heme binding	NAS Non-traceable Author Statement more info	PubMed
enables nitric-oxide synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nitric-oxide synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitric-oxide synthase activity	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables nitric-oxide synthase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables nitric-oxide synthase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in arginine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in imaginal disc development	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of DNA replication	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of cell population proliferation	TAS Traceable Author Statement more info	PubMed
involved_in nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nitric oxide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in nitric oxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nitric oxide biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in nitric oxide mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of heart rate	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of organ growth	TAS Traceable Author Statement more info	PubMed
involved_in response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hormone	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to lipopolysaccharide	IBA Inferred from Biological aspect of Ancestor more info
involved_in synapse assembly	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisome	ISS Inferred from Sequence or Structural Similarity more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: nitric oxide synthase

Names: CG6713-PA; CG6713-PF; CG6713-PK; NO synthase; Nos-PA; Nos-PF; Nos-PK; nitric oixide synthase; nitric oxidesynthase

NP_001027240.1

EC 1.-.-.-

EC 1.14.13.39

NP_001027243.2

EC 1.-.-.-

EC 1.14.13.39

NP_523541.2

EC 1.-.-.-

EC 1.14.13.39

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

10804274..10837511 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078817.4 → NP_523541.2 nitric oxide synthase, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523541.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V0K9, A4V0L0, Q27571, Q4ABG3, Q4ABG4, Q4ABG5, Q4ABG6, Q4ABG7, Q7YU33, Q9U096, Q9VKP8

Related

FBpp0079777

Conserved Domains (4) summary

cd06202
Location:931 → 1328

Nitric_oxide_synthase; The ferredoxin-reductase (FNR) like C-terminal domain of the nitric oxide synthase (NOS) fuses with a heme-containing N-terminal oxidase domain. The reductase portion is similar in structure to NADPH dependent cytochrome-450 reductase (CYPOR), having an ...

COG0369
Location:669 → 1323

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]

cd00795
Location:216 → 624

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:673 → 863

Flavodoxin_1; Flavodoxin
NM_001032069.2 → NP_001027240.1 nitric oxide synthase, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001027240.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q27571

Conserved Domains (2) summary

cd00795
Location:216 → 624

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:673 → 735

Flavodoxin_1; Flavodoxin
NM_001032072.2 → NP_001027243.2 nitric oxide synthase, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001027243.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q27571

Conserved Domains (4) summary

cd06202
Location:680 → 1077

Nitric_oxide_synthase; The ferredoxin-reductase (FNR) like C-terminal domain of the nitric oxide synthase (NOS) fuses with a heme-containing N-terminal oxidase domain. The reductase portion is similar in structure to NADPH dependent cytochrome-450 reductase (CYPOR), having an ...

COG0369
Location:418 → 1072

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]

cd00795
Location:3 → 373

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:422 → 612

Flavodoxin_1; Flavodoxin