Obsc Obscurin [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Obscprovided by FlyBase
Official Full Name: Obscurinprovided by FlyBase
Primary source: FLYBASE:FBgn0053519
Locus tag: Dmel_CG33519
See related: AllianceGenome:FB:FBgn0053519
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BEST:HL01080; CG18019; CG18020; CG18021; CG30171; CG30175; CG33519; CG3901; CT12925; CT40322; CT40326; Dmel\CG33519; dmObsc; obs; unc-89; Unc-89; UNC-89
Summary: Enables ATP binding activity; protein kinase binding activity; and tropomyosin binding activity. Involved in adult somatic muscle development; protein localization to M-band; and sarcomere organization. Located in M band. Is expressed in several structures, including adult heart; anterior-posterior subdivision of organism; somatic muscle cell; and spermatozoon. Human ortholog(s) of this gene implicated in centronuclear myopathy 5. Orthologous to human SPEG (striated muscle enriched protein kinase). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 60B1-60B2; 2-106 cM

Exon count:: 39

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (23995543..24014254, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (19883066..19901777, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Unlike obscurin in vertebrate skeletal muscle, Drosophila obscurin is necessary for the correct assembly of the IFM sarcomere.
Title: Binding partners of the kinase domains in Drosophila obscurin and their effect on the structure of the flight muscle.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D1S3696 (e-PCR)
- UniSTS:474274

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
NOT enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
NOT enables protein kinase activity	IKR Inferred from Key Residues more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables tropomyosin binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in adult somatic muscle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to M-band	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in sarcomere organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in M band	HDA more info	PubMed
located_in M band	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: obscurin

Names: CG33519-PC; CG33519-PD; CG33519-PE; CG33519-PF; CG33519-PG; CG33519-PH; Obsc-PC; Obsc-PD; Obsc-PE; Obsc-PF; Obsc-PG; Obsc-PH; PROJECTIN-like; Unc-89

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

23995543..24014254 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001299877.1 → NP_001286806.1 obscurin, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286806.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A8DYP0

Conserved Domains (10) summary

PHA03247
Location:493 → 714

PHA03247; large tegument protein UL36; Provisional

cd00063
Location:3433 → 3525

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd00160
Location:90 → 260

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13325
Location:275 → 388

PH_unc89; unc89 pleckstrin homology (PH) domain

cd14109
Location:2867 → 3125

PK_Unc-89_rpt1; Pseudokinase domain, first repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cd14112
Location:3578 → 3836

STKc_Unc-89_rpt2; Catalytic kinase domain, second repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

pfam07679
Location:2402 → 2490

I-set; Immunoglobulin I-set domain

cd00096
Location:2419 → 2423

Ig; Ig strand B [structural motif]

pfam13927
Location:1797 → 1887

Ig_3; Immunoglobulin domain

cl11960
Location:2100 → 2191

Ig; Immunoglobulin domain
NM_001299874.1 → NP_001286803.1 obscurin, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286803.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A8DYP0

Conserved Domains (10) summary

cd00096
Location:1145 → 1212

Ig; Immunoglobulin domain

smart00408
Location:1518 → 1585

IGc2; Immunoglobulin C-2 Type

smart00220
Location:3161 → 3415

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd00063
Location:3723 → 3815

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd00160
Location:90 → 260

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam07679
Location:2692 → 2780

I-set; Immunoglobulin I-set domain

cd13325
Location:275 → 388

PH_unc89; unc89 pleckstrin homology (PH) domain

cd14109
Location:3157 → 3415

PK_Unc-89_rpt1; Pseudokinase domain, first repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cd14112
Location:3868 → 4126

STKc_Unc-89_rpt2; Catalytic kinase domain, second repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cl11960
Location:2703 → 2772

Ig; Immunoglobulin domain
NM_001103970.2 → NP_001097440.1 obscurin, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097440.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A0A0B4LGC9, A0A0B4LGE3, A0A0B4LGI5, A0A0B4LHF2, A8DYP0, Q1ZYJ8, Q59E65

Conserved Domains (10) summary

cd00063
Location:3748 → 3840

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd00160
Location:90 → 260

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam07679
Location:2717 → 2805

I-set; Immunoglobulin I-set domain

cd13325
Location:275 → 388

PH_unc89; unc89 pleckstrin homology (PH) domain

cd14109
Location:3182 → 3440

PK_Unc-89_rpt1; Pseudokinase domain, first repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cd14112
Location:3893 → 4151

STKc_Unc-89_rpt2; Catalytic kinase domain, second repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

pfam13927
Location:1406 → 1487

Ig_3; Immunoglobulin domain

pfam16031
Location:502 → 596

TonB_N; TonB N-terminal region

cl26464
Location:493 → 690

Atrophin-1; Atrophin-1 family

cl28434
Location:3654 → 3744

I-set; Immunoglobulin I-set domain
NM_001014545.3 → NP_001014545.3 obscurin, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001014545.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A8DYP0

Conserved Domains (10) summary

cd00096
Location:1146 → 1213

Ig; Immunoglobulin domain

smart00408
Location:1519 → 1586

IGc2; Immunoglobulin C-2 Type

smart00220
Location:3162 → 3416

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd00063
Location:3724 → 3816

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd00160
Location:91 → 261

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam07679
Location:2693 → 2781

I-set; Immunoglobulin I-set domain

cd13325
Location:276 → 389

PH_unc89; unc89 pleckstrin homology (PH) domain

cd14109
Location:3158 → 3416

PK_Unc-89_rpt1; Pseudokinase domain, first repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cd14112
Location:3869 → 4127

STKc_Unc-89_rpt2; Catalytic kinase domain, second repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cl11960
Location:2704 → 2773

Ig; Immunoglobulin domain
NM_001299876.1 → NP_001286805.1 obscurin, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286805.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A8DYP0

Conserved Domains (8) summary

cd00096
Location:237 → 304

Ig; Immunoglobulin domain

smart00408
Location:610 → 677

IGc2; Immunoglobulin C-2 Type

smart00220
Location:2278 → 2532

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd00063
Location:2840 → 2932

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:1809 → 1897

I-set; Immunoglobulin I-set domain

cd14109
Location:2274 → 2532

PK_Unc-89_rpt1; Pseudokinase domain, first repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cd14112
Location:2985 → 3243

STKc_Unc-89_rpt2; Catalytic kinase domain, second repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cl11960
Location:1820 → 1889

Ig; Immunoglobulin domain
NM_001299875.1 → NP_001286804.1 obscurin, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286804.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A8DYP0

Conserved Domains (9) summary

cd00096
Location:892 → 959

Ig; Immunoglobulin domain

smart00408
Location:1265 → 1332

IGc2; Immunoglobulin C-2 Type

smart00220
Location:2933 → 3187

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd00063
Location:3495 → 3587

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:2464 → 2552

I-set; Immunoglobulin I-set domain

cd13325
Location:22 → 135

PH_unc89; unc89 pleckstrin homology (PH) domain

cd14109
Location:2929 → 3187

PK_Unc-89_rpt1; Pseudokinase domain, first repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cd14112
Location:3640 → 3898

STKc_Unc-89_rpt2; Catalytic kinase domain, second repeat, of the Giant Serine/Threonine Kinase Uncoordinated protein 89

cl11960
Location:2475 → 2544

Ig; Immunoglobulin domain