FAD3 fatty acid desaturase 3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: FAD3
Official Full Name: fatty acid desaturase 3
Primary source: TAIR:AT2G29980
Locus tag: AT2G29980
See related: Araport:AT2G29980
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtFAD3; ENDOPLASMIC RETICULUM 18:2 DESATURASE; F23F1.10; F23F1_10; fatty acid desaturase 3; OMEGA-3 FATTY ACID DESATURASE
Summary: Endoplasmic reticulum enzyme responsible for the synthesis of 18:3 fatty acids from phospholipids. Uses cytochrome b5 as electron donor.
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Genomic context

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See FAD3 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 7

Sequence:: Chromosome: 2; NC_003071.7 (12781524..12785190, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inter-Organellar Effects of Defective ER-Localized Linolenic Acid Formation on Thylakoid Lipid Composition, Non-Photochemical Quenching of Chlorophyll Fluorescence and Xanthophyll Cycle Activity in the Arabidopsis fad3 Mutant.
Title: Inter-Organellar Effects of Defective ER-Localized Linolenic Acid Formation on Thylakoid Lipid Composition, Non-Photochemical Quenching of Chlorophyll Fluorescence and Xanthophyll Cycle Activity in the Arabidopsis fad3 Mutant.
Histone acetyltransferase general control non-repressed protein 5 (GCN5) affects the fatty acid composition of Arabidopsis thaliana seeds by acetylating fatty acid desaturase3 (FAD3).
Title: Histone acetyltransferase general control non-repressed protein 5 (GCN5) affects the fatty acid composition of Arabidopsis thaliana seeds by acetylating fatty acid desaturase3 (FAD3).
ALA10 interacts with FATTY ACID DESATURASE2 (FAD2), and modification of ALA10 expression affects phosphatidylcholine (PC) fatty acyl desaturation by disturbing the balance between FAD2 and FAD3 activities.
Title: ALA10, a Phospholipid Flippase, Controls FAD2/FAD3 Desaturation of Phosphatidylcholine in the ER and Affects Chloroplast Lipid Composition in Arabidopsis thaliana.
SAD6 functions as a Delta9-desaturase, and together with FAD3 it increases the levels of unsaturated fatty acids in crown galls under hypoxia and drought stress conditions.
Title: Two fatty acid desaturases, STEAROYL-ACYL CARRIER PROTEIN Δ9-DESATURASE6 and FATTY ACID DESATURASE3, are involved in drought and hypoxia stress signaling in Arabidopsis crown galls.
FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channel.
Title: FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channeling in vivo.
that bZIP67 binds G-boxes in the FAD3 promoter and enhances FAD3 expression.
Title: bZIP67 regulates the omega-3 fatty acid content of Arabidopsis seed oil by activating fatty acid desaturase3.
Increase in the dosage of FAD3 results in an increase in the linolenic acid content of seed oil.
Title: Two high linolenic mutants of Arabidopsis thaliana contain megabase-scale genome duplications encompassing the FAD3 locus.
The polymethylene-interrupted dienoic fatty acid, mangiferic acid, can be produced in seed oil through the overexpression of a fatty acid n-3 desaturase.
Title: Overexpression of a FAD3 desaturase increases synthesis of a polymethylene-interrupted dienoic fatty acid in seeds of Arabidopsis thaliana L.
The fatty acid composition of membrane lipids influences cell growth and mitochondrial respiration, in particular the expression and capacity of alternative oxidase, under cold stress in fad2 knockout and FDAD3 overexpressing cell lines
Title: Alternative oxidase involvement in cold stress response of Arabidopsis thaliana fad2 and FAD3+ cell suspensions altered in membrane lipid composition.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_128552.4

Supported by ESTs:

NM_179808.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables acyl-lipid omega-3 desaturase (cytochrome b5) activity	IEA Inferred from Electronic Annotation more info
enables omega-3 fatty acid desaturase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables omega-3 fatty acid desaturase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in unsaturated fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within unsaturated fatty acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in endoplasmic reticulum	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: fatty acid desaturase 3

NP_180559.1

fatty acid desaturase 3 (FAD3); CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 8 (TAIR:AT5G05580.1); Has 2578 Blast hits to 2575 proteins in 659 species: Archae - 0; Bacteria - 965; Metazoa - 19; Fungi - 262; Plants - 918; Viruses - 0; Other Eukaryotes - 414 (source: NCBI BLink).

NP_850139.1

fatty acid desaturase 3 (FAD3); CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 8 (TAIR:AT5G05580.1); Has 2074 Blast hits to 2074 proteins in 524 species: Archae - 0; Bacteria - 643; Metazoa - 17; Fungi - 254; Plants - 868; Viruses - 0; Other Eukaryotes - 292 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

12781524..12785190 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_128552.4 → NP_180559.1 fatty acid desaturase 3 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_180559.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P48623, Q0WM09

UniProtKB/TrEMBL

A0A178VX43, A0A5S9X2L1

Conserved Domains (1) summary

PLN02498
Location:12 → 382

PLN02498; omega-3 fatty acid desaturase
NM_179808.2 → NP_850139.1 fatty acid desaturase 3 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850139.1

Status: REVIEWED

UniProtKB/TrEMBL

F4ILQ4

Conserved Domains (1) summary

cl00615
Location:28 → 274

Membrane-FADS-like; The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs, alkane hydroxylases, beta carotene ketolases (CrtW-like), hydroxylases (CrtR-like), and other related proteins. They are present in all groups of organisms with the ...