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    PREP2 presequence protease 2 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 841387, updated on 18-Sep-2024

    Summary

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    Official Symbol
    PREP2
    Official Full Name
    presequence protease 2
    Primary source
    TAIR:AT1G49630
    Locus tag
    AT1G49630
    See related
    Araport:AT1G49630
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATPREP2; F14J22.13; F14J22_13; presequence protease 2
    Summary
    Zinc metalloprotease pitrilysin subfamily A. Signal peptide degrading enzyme targeted to mitochondria and chloroplasts. Expressed in flower, leaf and root. Not expressed in silique and shoot.
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    Genomic context

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    See PREP2 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    20
    Sequence:
    Chromosome: 1; NC_003070.9 (18367590..18375494, complement)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene Cyclin-dependent kinase inhibitor family protein Neighboring gene alpha/beta-Hydrolases superfamily protein Neighboring gene alpha/beta-Hydrolases superfamily protein Neighboring gene carboxyesterase 5

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Phenotypical consequences of expressing the dually targeted Presequence Protease, AtPreP, exclusively in mitochondria. Kmiec B, et al. Biochimie, 2014 May. PMID 24373893
    2. Two novel targeting peptide degrading proteases, PrePs, in mitochondria and chloroplasts, so similar and still different. Ståhl A, et al. J Mol Biol, 2005 Jun 17. PMID 15893767
    3. Deletion of an organellar peptidasome PreP affects early development in Arabidopsis thaliana. Nilsson Cederholm S, et al. Plant Mol Biol, 2009 Nov. PMID 19701724
    4. Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2. Glaser E, et al. Biol Chem, 2006 Oct-Nov. PMID 17081117
    5. Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2. Bhushan S, et al. Plant Cell Physiol, 2005 Jun. PMID 15827031

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Exclusive mitochondrial expression of AtPreP results in total restoration of the proteolytic activity, but it does not restore the wild-type phenotype.
      Title: Phenotypical consequences of expressing the dually targeted Presequence Protease, AtPreP, exclusively in mitochondria.
    2. Data show that the AtPreP presequence has the capacity to target GFP into the mitochondrial matrix of all three species.
      Title: Targeting capacity and conservation of PreP homologues localization in mitochondria of different species.
    3. The AtPreP peptidasome is required for efficient plant growth and organelle function particularly during early development.
      Title: Deletion of an organellar peptidasome PreP affects early development in Arabidopsis thaliana.
    4. AtPreP1 and AtPreP2 genes are differentially expressed in Arabidopsis
      Title: Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2.
    5. AtPrePI and AtPrePII are metalloproteases that degrade targeting peptides in mitochondria and chloroplasts. [AtPrePII]
      Title: Two novel targeting peptide degrading proteases, PrePs, in mitochondria and chloroplasts, so similar and still different.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_001333396.1
    NM_001333397.1
    NM_103851.3
    NM_180630.2
    NM_180631.2

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within protein processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion ISM
    Inferred from Sequence Model
    more info
    		  
    located_in nucleus HDA PubMed 

    General protein information

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    Preferred Names
    presequence protease 2
    NP_175386.2
    • presequence protease 2 (PREP2); FUNCTIONS IN: metallopeptidase activity, metalloendopeptidase activity, zinc ion binding, catalytic activity, metal ion binding; INVOLVED IN: proteolysis, protein maturation by peptide bond cleavage; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase M16, C-terminal (InterPro:IPR007863), Peptidase M16C associated (InterPro:IPR013578), Peptidase M16, N-terminal (InterPro:IPR011765), Metalloenzyme, LuxS/M16 peptidase-like, metal-binding (InterPro:IPR011249), Peptidase M16, core (InterPro:IPR011237); BEST Arabidopsis thaliana protein match is: presequence protease 1 (TAIR:AT3G19170.1); Has 3964 Blast hits to 3937 proteins in 1352 species: Archae - 7; Bacteria - 2621; Metazoa - 205; Fungi - 264; Plants - 79; Viruses - 0; Other Eukaryotes - 788 (source: NCBI BLink).
    NP_850961.1
    • presequence protease 2 (PREP2); FUNCTIONS IN: metallopeptidase activity, metalloendopeptidase activity, zinc ion binding, catalytic activity, metal ion binding; INVOLVED IN: proteolysis, protein maturation by peptide bond cleavage; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase M16, C-terminal (InterPro:IPR007863), Peptidase M16C associated (InterPro:IPR013578), Peptidase M16, N-terminal (InterPro:IPR011765), Metalloenzyme, LuxS/M16 peptidase-like, metal-binding (InterPro:IPR011249), Peptidase M16, core (InterPro:IPR011237); BEST Arabidopsis thaliana protein match is: presequence protease 1 (TAIR:AT3G19170.1); Has 3964 Blast hits to 3937 proteins in 1352 species: Archae - 7; Bacteria - 2621; Metazoa - 205; Fungi - 264; Plants - 79; Viruses - 0; Other Eukaryotes - 788 (source: NCBI BLink).
    NP_850962.1
    • presequence protease 2 (PREP2); FUNCTIONS IN: metallopeptidase activity, metalloendopeptidase activity, catalytic activity, zinc ion binding, metal ion binding; INVOLVED IN: proteolysis, protein maturation by peptide bond cleavage; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase M16, C-terminal (InterPro:IPR007863), Peptidase M16C associated (InterPro:IPR013578), Peptidase M16, N-terminal (InterPro:IPR011765), Metalloenzyme, LuxS/M16 peptidase-like, metal-binding (InterPro:IPR011249), Peptidase M16, core (InterPro:IPR011237); BEST Arabidopsis thaliana protein match is: presequence protease 1 (TAIR:AT3G19170.1); Has 3964 Blast hits to 3937 proteins in 1352 species: Archae - 7; Bacteria - 2621; Metazoa - 205; Fungi - 264; Plants - 79; Viruses - 0; Other Eukaryotes - 788 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      18367590..18375494 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_180630.2 → NP_850961.1  presequence protease 2 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850961.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q8VY06, Q9FX91
      UniProtKB/TrEMBL
      A0A5S9WKE7
      Conserved Domains (4) summary
      COG1026
      Location:105 → 1078
      Cym1; Zn-dependent peptidase, M16 (insulinase) family [Posttranslational modification, protein turnover, chaperones]
      pfam00675
      Location:153 → 228
      Peptidase_M16; Insulinase (Peptidase family M16)
      pfam05193
      Location:300 → 486
      Peptidase_M16_C; Peptidase M16 inactive domain
      pfam08367
      Location:567 → 811
      M16C_assoc; Peptidase M16C associated

    2. NM_180631.2 → NP_850962.1  presequence protease 2 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850962.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q8VY06, Q9FX91
      UniProtKB/TrEMBL
      A0A5S9WKE7
      Conserved Domains (4) summary
      COG1026
      Location:105 → 1078
      Cym1; Zn-dependent peptidase, M16 (insulinase) family [Posttranslational modification, protein turnover, chaperones]
      pfam00675
      Location:153 → 228
      Peptidase_M16; Insulinase (Peptidase family M16)
      pfam05193
      Location:300 → 486
      Peptidase_M16_C; Peptidase M16 inactive domain
      pfam08367
      Location:567 → 811
      M16C_assoc; Peptidase M16C associated

    3. NM_103851.3 → NP_175386.2  presequence protease 2 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_175386.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q8VY06, Q9FX91
      UniProtKB/TrEMBL
      A0A5S9WKE7
      Conserved Domains (4) summary
      COG1026
      Location:105 → 1078
      Cym1; Zn-dependent peptidase, M16 (insulinase) family [Posttranslational modification, protein turnover, chaperones]
      pfam00675
      Location:153 → 228
      Peptidase_M16; Insulinase (Peptidase family M16)
      pfam05193
      Location:300 → 486
      Peptidase_M16_C; Peptidase M16 inactive domain
      pfam08367
      Location:567 → 811
      M16C_assoc; Peptidase M16C associated

    4. NM_001333396.1 → NP_001320455.1  presequence protease 2 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178W3J5, A0A1P8AMU8

    5. NM_001333397.1 → NP_001320454.1  presequence protease 2 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1P8AMX1, A0A5S9WKE7
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8VY06.1

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