Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pfkfb4provided by MGI
Official Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4provided by MGI
Primary source: MGI:MGI:2687284
See related: Ensembl:ENSMUSG00000025648 AllianceGenome:MGI:2687284
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: C230090D14
Summary: Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in fructose 2,6-bisphosphate metabolic process. Predicted to be active in cytosol. Is expressed in several structures, including eye; genitourinary system; gut; nervous system; and respiratory system. Orthologous to human PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in large intestine adult (RPKM 18.1), small intestine adult (RPKM 17.4) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F2; 9 59.63 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (108817336..108861302)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (108988272..109032236)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness.
Title: PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness.
Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
Title: Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
Inhibition of PFKFB4 suppresses glycolysis and proliferation of multiple human cancer cell lines.
Title: Targeting the sugar metabolism of tumors with a first-in-class 6-phosphofructo-2-kinase (PFKFB4) inhibitor.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (2)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 6-phosphofructo-2-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 6-phosphofructo-2-kinase activity	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IEA Inferred from Electronic Annotation more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in carbohydrate phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in fructose 2,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 2,6-bisphosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in fructose metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4

Names: 6PF-2-K/Fru-2,6-P2ase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; PFK/FBPase 4

NP_766607.3

EC 2.7.1.105

EC 3.1.3.46

XP_006512196.1

EC 2.7.1.105

EC 3.1.3.46

XP_017168891.1

EC 2.7.1.105

EC 3.1.3.46

XP_017168892.1

EC 2.7.1.105

EC 3.1.3.46

XP_017168893.1

EC 2.7.1.105

EC 3.1.3.46

XP_030100264.1

EC 2.7.1.105

EC 3.1.3.46

XP_036010927.1

EC 2.7.1.105

EC 3.1.3.46

XP_036010928.1

EC 2.7.1.105

EC 3.1.3.46

XP_036010929.1

EC 2.7.1.105

EC 3.1.3.46

XP_036010930.1

EC 2.7.1.105

EC 3.1.3.46

XP_036010931.1

EC 2.7.1.105

EC 3.1.3.46

XP_036010932.1

EC 2.7.1.105

EC 3.1.3.46

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001431581.1 → NP_001418510.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform 2

Status: VALIDATED

Source sequence(s)

AC140383
NM_001431582.1 → NP_001418511.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform 3

Status: VALIDATED

Source sequence(s)

AC140383
NM_173019.6 → NP_766607.3 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform 1

See identical proteins and their annotated locations for NP_766607.3

Status: VALIDATED

Source sequence(s)

AC140383

Consensus CDS

CCDS23541.1

UniProtKB/Swiss-Prot

Q5UD55, Q5UD56, Q5UD57, Q5Y296, Q6DTY7, Q6PFE8

Related

ENSMUSP00000142378.2, ENSMUST00000198140.5

Conserved Domains (2) summary

pfam01591
Location:35 → 249

6PF2K; 6-phosphofructo-2-kinase

pfam00300
Location:252 → 435

His_Phos_1; Histidine phosphatase superfamily (branch 1)

RNA

NR_153429.2 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC140383
NR_153430.2 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC140383

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

108817336..108861302

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155037.1 → XP_036010930.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X4

Conserved Domains (2) summary

pfam00300
Location:305 → 477

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:24 → 302

6PF2K; 6-phosphofructo-2-kinase
XM_036155035.1 → XP_036010928.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X2

Conserved Domains (2) summary

pfam00300
Location:308 → 480

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:27 → 305

6PF2K; 6-phosphofructo-2-kinase
XM_036155038.1 → XP_036010931.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X5

Conserved Domains (2) summary

pfam00300
Location:294 → 466

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:13 → 291

6PF2K; 6-phosphofructo-2-kinase
XM_006512133.3 → XP_006512196.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X6

Conserved Domains (2) summary

pfam01591
Location:35 → 249

6PF2K; 6-phosphofructo-2-kinase

pfam00300
Location:252 → 431

His_Phos_1; Histidine phosphatase superfamily (branch 1)
XM_036155036.1 → XP_036010929.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

Conserved Domains (2) summary

pfam00300
Location:316 → 502

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:30 → 313

6PF2K; 6-phosphofructo-2-kinase
XM_036155034.1 → XP_036010927.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X1

Conserved Domains (2) summary

pfam00300
Location:316 → 488

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:30 → 313

6PF2K; 6-phosphofructo-2-kinase
XM_030244404.2 → XP_030100264.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X7

Conserved Domains (2) summary

pfam01591
Location:1 → 215

6PF2K; 6-phosphofructo-2-kinase

pfam00300
Location:218 → 397

His_Phos_1; Histidine phosphatase superfamily (branch 1)
XM_017313402.3 → XP_017168891.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X8

UniProtKB/TrEMBL

C9VZF2

Conserved Domains (2) summary

pfam01591
Location:1 → 215

6PF2K; 6-phosphofructo-2-kinase

pfam00300
Location:218 → 401

His_Phos_1; Histidine phosphatase superfamily (branch 1)
XM_017313404.2 → XP_017168893.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X10

UniProtKB/TrEMBL

C9VZF3

Conserved Domains (2) summary

pfam00300
Location:61 → 244

His_Phos_1; Histidine phosphatase superfamily (branch 1)

cl17190
Location:1 → 58

NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...
XM_036155039.1 → XP_036010932.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X11

Conserved Domains (2) summary

pfam00300
Location:34 → 206

His_Phos_1; Histidine phosphatase superfamily (branch 1)

cl17190
Location:1 → 31

NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...
XM_017313403.2 → XP_017168892.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X9

Conserved Domains (2) summary

pfam00300
Location:61 → 240

His_Phos_1; Histidine phosphatase superfamily (branch 1)

cl17190
Location:1 → 58

NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_001039215.1: Suppressed sequence

Description

NM_001039215.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
NM_001039216.1: Suppressed sequence

Description

NM_001039216.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
NM_001039217.1: Suppressed sequence

Description

NM_001039217.1: This RefSeq was removed because currently there is support for the transcript but not for the protein.