Slc19a1 solute carrier family 19 (folate transporter), member 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slc19a1provided by MGI
Official Full Name: solute carrier family 19 (folate transporter), member 1provided by MGI
Primary source: MGI:MGI:103182
See related: Ensembl:ENSMUSG00000001436 AllianceGenome:MGI:103182
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: RFC; RFC1; RFC-1
Summary: Enables folic acid transmembrane transporter activity and methotrexate transmembrane transporter activity. Involved in folate import across plasma membrane and methotrexate transport. Located in apical plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including abdominal aortic aneurysm; cleft lip; dextro-looped transposition of the great arteries; hematologic cancer; and malaria. Orthologous to human SLC19A1 (solute carrier family 19 member 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in kidney adult (RPKM 62.7), ovary adult (RPKM 25.1) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 39.72 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (76868103..76886266)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (77032269..77050432)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Reduced folate carrier 1 is present in retinal microvessels and crucial for the inner blood retinal barrier integrity.
Title: Reduced folate carrier 1 is present in retinal microvessels and crucial for the inner blood retinal barrier integrity.
The authors identified the cellular protein used as a receptor by GLN Endogenous Retrovirus for cell entry as SLC19A1, the reduced folate carrier.
Title: Identification of the Receptor Used by the Ecotropic Mouse GLN Endogenous Retrovirus.
The mRNAs encoding Folr2 and the intestinal folate transporter Slc46a1 were not detected in 2-cel embryos and blastocysts.
Title: Folate transport in mouse cumulus-oocyte complexes and preimplantation embryos.
Interleukin-6 regulated the efficacy of methotrexate by decreasing the expression of SLC19A1.
Title: Interleukin-6 regulates anti-arthritic effect of methotrexate via reduction of SLC19A1 expression in a mouse arthritis model.
expressed in placenta at highest level during early development
Title: Spatial and temporal expression of folate-related transporters and metabolic enzymes during mouse placental development.
Intestinal folate uptake process undergoes differentiation-dependent regulation and that this regulation is mediated via changes in the level of expression of both the RFC and PCFT.
Title: Differentiation-dependent regulation of the intestinal folate uptake process: studies with Caco-2 cells and native mouse intestine.
Inactivation of RFC1 impacts the expression of several ligands and interacting proteins in the cubilin-amnionless-megalin complex that are involved in the maternal-fetal transport of folate and other nutrients.
Title: Microarray analysis of E9.5 reduced folate carrier (RFC1; Slc19a1) knockout embryos reveals altered expression of genes in the cubilin-megalin multiligand endocytic receptor complex.
Decreased expression of the reduced folate carrier and folypolyglutamate synthetase is the basis for acquired resistance to the pemetrexed antifolate (LY231514) in an L1210 murine leukemia cell line.
Title: Decreased expression of the reduced folate carrier and folypolyglutamate synthetase is the basis for acquired resistance to the pemetrexed antifolate (LY231514) in an L1210 murine leukemia cell line.
existence of four unique noncoding exons and promoters for mRFC and demonstrate a facile induction of mRNAs for mRFC and multidrug resistance-associated proteins 1 and 3 in intestine in response to changes in dietary folate intake
Title: Structure and regulation of the murine reduced folate carrier gene: identification of four noncoding exons and promoters and regulation by dietary folates.
First evidence of RFC expression in rapidly dividing cells of developing neural tube, craniofacial region, limb buds and heart duggests that these regions may be particularly susceptible to folate deficiency.
Title: Reduced-folate carrier (RFC) is expressed in placenta and yolk sac, as well as in cells of the developing forebrain, hindbrain, neural tube, craniofacial region, eye, limb buds and heart.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (2) 1 citation

General gene information

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Markers

Cltc (e-PCR), detects polymorphism
- UniSTS:259594

U32469 (e-PCR)
- UniSTS:125722

Slc19a1 (e-PCR), detects polymorphism
- UniSTS:259141

PMC184426P1 (e-PCR)
- UniSTS:271687

AI323572 (e-PCR)
- UniSTS:180060

U66103 (e-PCR)
- UniSTS:163478

NoName (e-PCR)
- UniSTS:548589

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 2',3'-cyclic GMP-AMP binding	IEA Inferred from Electronic Annotation more info
enables 2',3'-cyclic GMP-AMP binding	ISO Inferred from Sequence Orthology more info
enables antiporter activity	ISO Inferred from Sequence Orthology more info
enables antiporter activity	ISS Inferred from Sequence or Structural Similarity more info
NOT enables cyclic-GMP-AMP transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables cyclic-GMP-AMP transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables folate:monoatomic anion antiporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables folate:monoatomic anion antiporter activity	ISO Inferred from Sequence Orthology more info
enables folate:monoatomic anion antiporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables folic acid binding	IBA Inferred from Biological aspect of Ancestor more info
enables folic acid binding	IEA Inferred from Electronic Annotation more info
enables folic acid binding	ISO Inferred from Sequence Orthology more info
enables folic acid transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables methotrexate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables methotrexate transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables organic anion transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables xenobiotic transmembrane transporter activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
NOT involved_in cyclic-GMP-AMP transmembrane import across plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in cyclic-GMP-AMP transmembrane import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in female pregnancy	IEA Inferred from Electronic Annotation more info
involved_in folate import across plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in folate import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in folate transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in folate transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in folate transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in folic acid transport	ISO Inferred from Sequence Orthology more info
involved_in methotrexate transport	IDA Inferred from Direct Assay more info	PubMed
involved_in methotrexate transport	ISO Inferred from Sequence Orthology more info
involved_in monoatomic anion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in organic anion transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cGAS/STING signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cGAS/STING signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in xenobiotic transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in xenobiotic transmembrane transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in basolateral plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in brush border membrane	IEA Inferred from Electronic Annotation more info
located_in brush border membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: reduced folate transporter

Names: IFC-1; folate transporter 1; intestinal folate carrier 1; intestinal folate carrier protein; plasma membrane folate antiporter SLC19A1; reduced folate carrier 1; solute carrier family 19 (sodium/hydrogen exchanger), member 1; solute carrier family 19 member 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001199271.2 → NP_001186200.1 reduced folate transporter isoform a

See identical proteins and their annotated locations for NP_001186200.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains an alternate exon for its 5' UTR, compared to variant 1. Variants 1-4 all encode the same isoform (a).

Source sequence(s)

AC055777

Consensus CDS

CCDS35947.1

UniProtKB/Swiss-Prot

P41438, Q62450, Q62451

UniProtKB/TrEMBL

Q542F3, Q9DCR3

Related

ENSMUSP00000116784.2, ENSMUST00000144234.8

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
NM_001428820.1 → NP_001415749.1 reduced folate transporter isoform a

Status: VALIDATED

Source sequence(s)

AC055777

UniProtKB/Swiss-Prot

P41438, Q62450, Q62451

UniProtKB/TrEMBL

Q542F3
NM_001428821.1 → NP_001415750.1 reduced folate transporter isoform a

Status: VALIDATED

Source sequence(s)

AC055777

UniProtKB/Swiss-Prot

P41438, Q62450, Q62451

UniProtKB/TrEMBL

Q542F3
NM_001428822.1 → NP_001415751.1 reduced folate transporter isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (5), as well as variant 6, encodes isoform b.

Source sequence(s)

AC055777
NM_001428823.1 → NP_001415752.1 reduced folate transporter isoform b

Status: VALIDATED

Source sequence(s)

AC055777
NM_001428824.1 → NP_001415753.1 reduced folate transporter isoform c

Status: VALIDATED

Source sequence(s)

AC055777
NM_001428825.1 → NP_001415754.1 reduced folate transporter isoform d

Status: VALIDATED

Description

Transcript Variant: This variant (8), as well as variant 9, encodes isoform d.

Source sequence(s)

AC055777
NM_001428826.1 → NP_001415755.1 reduced folate transporter isoform d

Status: VALIDATED

Source sequence(s)

AC055777
NM_001428827.1 → NP_001415756.1 reduced folate transporter isoform e

Status: VALIDATED

Source sequence(s)

AC055777
NM_001428828.1 → NP_001415757.1 reduced folate transporter isoform f

Status: VALIDATED

Source sequence(s)

AC055777
NM_031196.4 → NP_112473.1 reduced folate transporter isoform a

See identical proteins and their annotated locations for NP_112473.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1-4 all encode the same isoform (a).

Source sequence(s)

AC055777

Consensus CDS

CCDS35947.1

UniProtKB/Swiss-Prot

P41438, Q62450, Q62451

UniProtKB/TrEMBL

Q542F3, Q9DCR3

Related

ENSMUSP00000101050.4, ENSMUST00000105410.10

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier

RNA

NR_190499.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190500.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190501.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190502.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190503.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190504.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190505.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190506.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190507.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190508.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190509.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777
NR_190510.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC055777

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

76868103..76886266

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006513416.4 → XP_006513479.1 reduced folate transporter isoform X1

See identical proteins and their annotated locations for XP_006513479.1

UniProtKB/Swiss-Prot

P41438, Q62450, Q62451

UniProtKB/TrEMBL

Q542F3, Q9DCR3

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_006513417.4 → XP_006513480.1 reduced folate transporter isoform X1

See identical proteins and their annotated locations for XP_006513480.1

UniProtKB/Swiss-Prot

P41438, Q62450, Q62451

UniProtKB/TrEMBL

Q542F3, Q9DCR3

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_030244971.2 → XP_030100831.1 reduced folate transporter isoform X2

Conserved Domains (1) summary

cl27502
Location:1 → 273

Folate_carrier; Reduced folate carrier
XM_006513414.5 → XP_006513477.1 reduced folate transporter isoform X1

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_006513419.5 → XP_006513482.1 reduced folate transporter isoform X2

Conserved Domains (1) summary

cl27502
Location:1 → 273

Folate_carrier; Reduced folate carrier
XM_036155714.1 → XP_036011607.1 reduced folate transporter isoform X3

Conserved Domains (1) summary

cl27502
Location:1 → 273

Folate_carrier; Reduced folate carrier
XM_006513415.5 → XP_006513478.1 reduced folate transporter isoform X1

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier
XM_036155713.1 → XP_036011606.1 reduced folate transporter isoform X1

UniProtKB/Swiss-Prot

P41438, Q62450, Q62451

UniProtKB/TrEMBL

Q542F3, Q9DCR3

Conserved Domains (1) summary

TIGR00806
Location:1 → 512

rfc; RFC reduced folate carrier