CHST4 carbohydrate sulfotransferase 4 [Homo sapiens (human)] - Gene

Summary

Official Symbol: CHST4provided by HGNC
Official Full Name: carbohydrate sulfotransferase 4provided by HGNC
Primary source: HGNC:HGNC:1972
See related: Ensembl:ENSG00000140835 AllianceGenome:HGNC:1972
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GST3; LSST; GlcNAc6ST2; HECGLCNAC6ST
Summary: This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]
Expression: Restricted expression toward gall bladder (RPKM 42.6) See more
Orthologs: mouse all
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Genomic context

Location:: 16q22.2

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	16	NC_000016.10 (71526120..71538748)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	16	NC_060940.1 (77343320..77355947)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	16	NC_000016.9 (71560023..71572651)

Chromosome 16 - NC_000016.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

mRNA transcript analyses on the genes involved in synthesizing GlcNAc-6-O-sulfated glycans in human colon cancer tissues indicated that GlcNAc6ST-2 (CHST4) is preferentially expressed in cancer cells ... GlcNAc6ST-3 (CHST5) was only expressed in nonmalignant epithelial cells, whereas GlcNAc6ST-1 (CHST2) was expressed equally in both cancerous and nonmalignant epithelial cells.
Title: Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry-based sulfoglycomic analysis.
Data suggest that GlcNAc6ST-2 (CHST4) is critical for biosynthesis of MECA-79 sulfated glycans in apical membranes of small-sized intrahepatic bile ducts and of cholangiolocellular carcinoma (CoCC) cells; MUC1 protein decorated with GlcNAc6ST-2-dependent MECA-79 sulfated glycans may serve as a useful CoCC marker.
Title: Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma.
A method for O-GlcNAc detection using in vitro sulfation with two N-acetylglucosamine (GlcNAc)-specific sulfotransferases, carbohydrate sulfotransferase 2 and carbohydrate sulfotransferase 4.
Title: Detecting O-GlcNAc using in vitro sulfation.
GlcNAc6ST2 could therefore be a good serological marker for detecting early-stage uterine cervical and corpus cancers.
Title: N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer.
Observational study of gene-disease association. (HuGE Navigator)
Title: Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects.
involved in enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate
Title: Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate.
Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma
Title: Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp160, precursor	env	Sulfate is linked to the carbohydrate chains of HIV-1 glycoproteins gp160, gp120, and gp41 during the endoplasmic reticulum to Golgi transport of the glycoproteins	PubMed

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables N-acetylglucosamine 6-O-sulfotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables N-acetylglucosamine 6-O-sulfotransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables N-acetylglucosamine 6-O-sulfotransferase activity	TAS Traceable Author Statement more info
enables sulfotransferase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in N-acetylglucosamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in N-acetylglucosamine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in O-glycan processing	TAS Traceable Author Statement more info
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in cell adhesion	TAS Traceable Author Statement more info	PubMed
involved_in cell-cell signaling	TAS Traceable Author Statement more info	PubMed
involved_in immune response	TAS Traceable Author Statement more info	PubMed
involved_in inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of leukocyte tethering or rolling	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein sulfation	TAS Traceable Author Statement more info	PubMed
involved_in sulfur compound metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in sulfur compound metabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	NAS Non-traceable Author Statement more info	PubMed
located_in Golgi membrane	TAS Traceable Author Statement more info
is_active_in trans-Golgi network	IBA Inferred from Biological aspect of Ancestor more info
located_in trans-Golgi network	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: carbohydrate sulfotransferase 4

Names: GST-3; HEC-GlcNAc6ST; L-selectin ligand sulfotransferase; N-acetylglucosamine 6-O-sulfotransferase 2; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; glcNAc6ST-2; gn6st-2; high endothelial cells N-acetylglucosamine 6-O-sulfotransferase

NP_001159867.1

EC 2.8.2.-

NP_005760.1

EC 2.8.2.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029801.1 RefSeqGene

Range

5307..17629

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001166395.2 → NP_001159867.1 carbohydrate sulfotransferase 4

See identical proteins and their annotated locations for NP_001159867.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AC010547, BC035282, DA045171

Consensus CDS

CCDS10902.1

UniProtKB/Swiss-Prot

Q8IV46, Q8NCG5, Q9Y5R3

Related

ENSP00000441204.3, ENST00000539698.4

Conserved Domains (1) summary

cl21551
Location:168 → 358

Sulfotransfer_1; Sulfotransferase domain
NM_005769.2 → NP_005760.1 carbohydrate sulfotransferase 4

See identical proteins and their annotated locations for NP_005760.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) and variant 2 encode the same protein.

Source sequence(s)

AC010547, AF131235, BC035282, DA496702

Consensus CDS

CCDS10902.1

UniProtKB/Swiss-Prot

Q8IV46, Q8NCG5, Q9Y5R3

Related

ENSP00000341206.5, ENST00000338482.5

Conserved Domains (1) summary

cl21551
Location:168 → 358

Sulfotransfer_1; Sulfotransferase domain