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    Dock8 dedicator of cytokinesis 8 [ Mus musculus (house mouse) ]

    Gene ID: 76088, updated on 10-Dec-2024

    Summary

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    Official Symbol
    Dock8provided by MGI
    Official Full Name
    dedicator of cytokinesis 8provided by MGI
    Primary source
    MGI:MGI:1921396
    See related
    Ensembl:ENSMUSG00000052085 AllianceGenome:MGI:1921396
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    1200017A24Rik; 5830472H07Rik; A130095G14Rik
    Summary
    Enables guanyl-nucleotide exchange factor activity. Involved in dendritic cell migration; positive regulation of T cell migration; and positive regulation of establishment of T cell polarity. Acts upstream of or within immunological synapse formation and negative regulation of T cell apoptotic process. Located in cell leading edge. Is expressed in central nervous system; retina; and thymus primordium. Human ortholog(s) of this gene implicated in atopic dermatitis; bacterial infectious disease; hyper IgE recurrent infection syndrome 2; immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome; and viral infectious disease (multiple). Orthologous to human DOCK8 (dedicator of cytokinesis 8). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Broad expression in thymus adult (RPKM 25.8), spleen adult (RPKM 17.9) and 18 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Dock8 in Genome Data Viewer
    Location:
    19 B; 19 19.91 cM
    Exon count:
    52
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (24976753..25179796)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (24999321..25202432)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_45808 Neighboring gene forkhead box D4 Neighboring gene STARR-positive B cell enhancer mm9_chr19:25033055-25033356 Neighboring gene STARR-positive B cell enhancer ABC_E7639 Neighboring gene microRNA 3084-1 Neighboring gene STARR-positive B cell enhancer ABC_E5693 Neighboring gene Zn regulated GTPase metalloprotein activator 1 Neighboring gene STARR-seq mESC enhancer starr_45809 Neighboring gene CapStarr-seq enhancer MGSCv37_chr19:25126893-25127076 Neighboring gene STARR-positive B cell enhancer ABC_E1974 Neighboring gene NADH:ubiquinone oxidoreductase subunit B4B Neighboring gene predicted gene, 34235 Neighboring gene STARR-seq mESC enhancer starr_45812 Neighboring gene predicted gene, 34186 Neighboring gene predicted gene, 24252 Neighboring gene STARR-seq mESC enhancer starr_45817 Neighboring gene KN motif and ankyrin repeat domains 1 Neighboring gene STARR-seq mESC enhancer starr_45819 Neighboring gene predicted gene, 34432 Neighboring gene predicted gene, 52392

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Protection from EAE in DOCK8 mutant mice occurs despite increased Th17 cell frequencies in the periphery. Wilson AS, et al. Eur J Immunol, 2019 May. PMID 30729501
    2. DOCK8 is essential for neutrophil mediated clearance of cutaneous S. aureus infection. Wilkie H, et al. Clin Immunol, 2023 Sep. PMID 37385324, Free PMC Article
    3. Dock8 mutations cripple B cell immunological synapses, germinal centers and long-lived antibody production. Randall KL, et al. Nat Immunol, 2009 Dec. PMID 19898472, Free PMC Article
    4. DOCK8 is expressed in microglia, and it regulates microglial activity during neurodegeneration in murine disease models. Namekata K, et al. J Biol Chem, 2019 Sep 6. PMID 31337702, Free PMC Article
    5. IL-21 Rescues the Defect of IL-10-Producing Regulatory B Cells and Improves Allergic Asthma in DOCK8 Deficient Mice. Jiang J, et al. Front Immunol, 2021. PMID 34867940, Free PMC Article

    See all (61) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Regulatory T-cell dysfunction and cutaneous exposure to Staphylococcus aureus underlie eczema in DOCK8 deficiency.
      Title: Regulatory T-cell dysfunction and cutaneous exposure to Staphylococcus aureus underlie eczema in DOCK8 deficiency.
    2. Metabolic fitness of IgA[+] plasma cells in the gut requires DOCK8.
      Title: Metabolic fitness of IgA(+) plasma cells in the gut requires DOCK8.
    3. MYD88L265P augments proximal B-cell receptor signaling in large B-cell lymphomas via an interaction with DOCK8.
      Title: MYD88L265P augments proximal B-cell receptor signaling in large B-cell lymphomas via an interaction with DOCK8.
    4. DOCK8 is essential for neutrophil mediated clearance of cutaneous S. aureus infection.
      Title: DOCK8 is essential for neutrophil mediated clearance of cutaneous S. aureus infection.
    5. IL-21 Rescues the Defect of IL-10-Producing Regulatory B Cells and Improves Allergic Asthma in DOCK8 Deficient Mice.
      Title: IL-21 Rescues the Defect of IL-10-Producing Regulatory B Cells and Improves Allergic Asthma in DOCK8 Deficient Mice.
    6. Cutting Edge: DOCK8 Regulates a Subset of Dendritic Cells That Is Critical for the Development of Experimental Autoimmune Encephalomyelitis.
      Title: Cutting Edge: DOCK8 Regulates a Subset of Dendritic Cells That Is Critical for the Development of Experimental Autoimmune Encephalomyelitis.
    7. DOCK8 Expression in Regulatory T Cells Maintains their Stability and Limits Contact Hypersensitivity.
      Title: DOCK8 Expression in Regulatory T Cells Maintains their Stability and Limits Contact Hypersensitivity.
    8. DOCK8 deficiency causes a skewing to type 2 immunity in the gut with expansion of group 2 innate lymphoid cells.
      Title: DOCK8 deficiency causes a skewing to type 2 immunity in the gut with expansion of group 2 innate lymphoid cells.
    9. Our studies reveal the novel finding that DOCK8 mutant mice, in contrast to the findings in humans, have elevated Th17 cells. Despite this, they do not get exaggerated EAE but appear protected due to a Th17 specific migration defect to the CNS.
      Title: Protection from EAE in DOCK8 mutant mice occurs despite increased Th17 cell frequencies in the periphery.
    10. The DOCK8 is expressed in microglia and regulates microglial activity in disease states.
      Title: DOCK8 is expressed in microglia, and it regulates microglial activity during neurodegeneration in murine disease models.
    Submit: New GeneRIF Correction See all GeneRIFs (23)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables guanyl-nucleotide exchange factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables guanyl-nucleotide exchange factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables guanyl-nucleotide exchange factor activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular response to chemokine IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cellular response to chemokine ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within dendritic cell migration IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within dendritic cell migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in dendritic cell migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within immunological synapse formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within memory T cell proliferation ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within negative regulation of T cell apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of GTPase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of T cell migration IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of T cell migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of T cell migration ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of establishment of T cell polarity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of establishment of T cell polarity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of establishment of T cell polarity ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of Rho protein signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in small GTPase-mediated signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cell leading edge IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell leading edge IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in lamellipodium membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in leading edge membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    dedicator of cytokinesis protein 8

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_028785.3 → NP_083061.2  dedicator of cytokinesis protein 8

      See identical proteins and their annotated locations for NP_083061.2

      Status: VALIDATED

      Source sequence(s)
      AK028968, AK038285, AK050543, AK131180, DT909592
      Consensus CDS
      CCDS37943.1
      UniProtKB/Swiss-Prot
      Q6KAM7, Q6PIS0, Q7TMQ5, Q8C147, Q8CAP6, Q8K105, Q9DBQ2
      UniProtKB/TrEMBL
      B9EJ70
      Related
      ENSMUSP00000025831.7, ENSMUST00000025831.8
      Conserved Domains (3) summary
      cd08696
      Location:560 → 740
      C2_Dock-C; C2 domains found in Dedicator Of CytoKinesis (Dock) class C proteins
      cd11701
      Location:1637 → 2058
      DHR2_DOCK8; Dock Homology Region 2, a GEF domain, of Class C Dedicator of Cytokinesis 8
      pfam11878
      Location:73 → 164
      DUF3398; Domain of unknown function (DUF3398)

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      24976753..25179796
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006527436.5 → XP_006527499.1  dedicator of cytokinesis protein 8 isoform X2

      Conserved Domains (2) summary
      cd08696
      Location:560 → 740
      C2_Dock-C; C2 domains found in Dedicator Of CytoKinesis (Dock) class C proteins
      pfam11878
      Location:56 → 166
      DUF3398; Domain of unknown function (DUF3398)

    2. XM_011247397.4 → XP_011245699.1  dedicator of cytokinesis protein 8 isoform X1

      See identical proteins and their annotated locations for XP_011245699.1

      UniProtKB/TrEMBL
      B9EJ70
      Conserved Domains (3) summary
      cd08696
      Location:492 → 672
      C2_Dock-C; C2 domains found in Dedicator Of CytoKinesis (Dock) class C proteins
      cd11701
      Location:1569 → 1990
      DHR2_DOCK8; Dock Homology Region 2, a GEF domain, of Class C Dedicator of Cytokinesis 8
      pfam11878
      Location:1 → 98
      DUF3398; Domain of unknown function (DUF3398)

    3. XM_006527435.3 → XP_006527498.1  dedicator of cytokinesis protein 8 isoform X1

      See identical proteins and their annotated locations for XP_006527498.1

      UniProtKB/TrEMBL
      B9EJ70
      Conserved Domains (3) summary
      cd08696
      Location:492 → 672
      C2_Dock-C; C2 domains found in Dedicator Of CytoKinesis (Dock) class C proteins
      cd11701
      Location:1569 → 1990
      DHR2_DOCK8; Dock Homology Region 2, a GEF domain, of Class C Dedicator of Cytokinesis 8
      pfam11878
      Location:1 → 98
      DUF3398; Domain of unknown function (DUF3398)

    RNA

    1. XR_004940182.1 RNA Sequence

    2. XR_004940183.1 RNA Sequence

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_175233.3: Suppressed sequence

      Description
      NM_175233.3: This RefSeq was temporarily suppressed because currently there are not sufficient data to support this transcript.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8C147.4 GenPept UniProtKB/Swiss-Prot:Q8C147

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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