NEK6 NIMA related kinase 6 [Homo sapiens (human)] - Gene

Summary

Official Symbol: NEK6provided by HGNC
Official Full Name: NIMA related kinase 6provided by HGNC
Primary source: HGNC:HGNC:7749
See related: Ensembl:ENSG00000119408 MIM:604884; AllianceGenome:HGNC:7749
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: SID6-1512
Summary: The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Expression: Ubiquitous expression in placenta (RPKM 13.1), appendix (RPKM 13.0) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 9q33.3

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	9	NC_000009.12 (124257606..124353307)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	9	NC_060933.1 (136455708..136551354)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	9	NC_000009.11 (127019885..127115586)

Chromosome 9 - NC_000009.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CRISPR/Cas9 screen in human iPSC-derived cortical neurons identifies NEK6 as a novel disease modifier of C9orf72 poly(PR) toxicity.
Title: CRISPR/Cas9 screen in human iPSC-derived cortical neurons identifies NEK6 as a novel disease modifier of C9orf72 poly(PR) toxicity.
NEK6 Regulates Redox Balance and DNA Damage Response in DU-145 Prostate Cancer Cells.
Title: NEK6 Regulates Redox Balance and DNA Damage Response in DU-145 Prostate Cancer Cells.
Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma.
Title: Predictive Role of NEK6 in Prognosis and Immune Infiltration in Head and Neck Squamous Cell Carcinoma.
NEK6 is an injury-responsive kinase cooperating with STAT3 in regulation of reactive astrogliosis.
Title: NEK6 is an injury-responsive kinase cooperating with STAT3 in regulation of reactive astrogliosis.
Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.
Title: Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.
CircNEK6 promoted the progression of thyroid cancer through up-regulating FZD8 and activating Wnt signaling pathway by targeting miR-370-3p.
Title: Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway.
our results imply that Nek6 plays a facilitating role in breast cancer cell proliferation
Title: Overexpression of NIMA-related kinase 6 (NEK6) contributes to malignant growth and dismal prognosis in Human Breast Cancer.
cis-regulatory circuits that lead to overexpression of NEK6, a mitosis-associated kinase, in human B cell lymphoma, were analyzed.
Title: cis-Regulatory Circuits Regulating NEK6 Kinase Overexpression in Transformed B Cells Are Super-Enhancer Independent.
High NEK6 expression is associated with Cluster Amplified Centrosomes in cancer.
Title: Hsp72 and Nek6 Cooperate to Cluster Amplified Centrosomes in Cancer Cells.
Signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14 to properly coordinate cytokinesis.
Title: A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase.

Submit: New GeneRIF Correction See all GeneRIFs (37)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Common genetic variation and performance on standardized cognitive tests. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH119892 (e-PCR)
- UniSTS:133636

RH11599 (e-PCR)
- UniSTS:61308

SHGC-154753 (e-PCR)
- UniSTS:184960

RH12239 (e-PCR)
- UniSTS:63110

SGC33089 (e-PCR)
- UniSTS:78220

RH77983 (e-PCR)
- UniSTS:226

RH11482 (e-PCR)
- UniSTS:58088

G20648 (e-PCR)
- UniSTS:68859

A006A17 (e-PCR)
- UniSTS:68860

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables kinesin binding	IPI Inferred from Physical Interaction more info	PubMed
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info
enables transcription corepressor binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome segregation	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic nuclear membrane disassembly	TAS Traceable Author Statement more info
involved_in mitotic spindle organization	TAS Traceable Author Statement more info
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	HMP more info	PubMed
involved_in protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cellular senescence	TAS Traceable Author Statement more info	PubMed
involved_in regulation of mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in regulation of mitotic metaphase/anaphase transition	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mitotic metaphase/anaphase transition	IDA Inferred from Direct Assay more info	PubMed
involved_in spindle assembly	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in centriolar satellite	IDA Inferred from Direct Assay more info
colocalizes_with centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in cytosol	TAS Traceable Author Statement more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine-protein kinase Nek6

Names: NIMA (never in mitosis gene a)-related kinase 6; never in mitosis A-related kinase 6; nimA-related protein kinase 6; protein kinase SID6-1512; putative serine-threonine protein kinase

NP_001138473.1

EC 2.7.11.34

NP_001159639.1

EC 2.7.11.34

NP_001159640.1

EC 2.7.11.34

NP_001159641.1

EC 2.7.11.34

NP_001159642.1

EC 2.7.11.34

NP_001159643.1

EC 2.7.11.34

NP_055212.2

EC 2.7.11.34

XP_016869706.1

EC 2.7.11.34

XP_024303155.1

EC 2.7.11.34

XP_047278607.1

EC 2.7.11.34

XP_047278608.1

EC 2.7.11.34

XP_047278609.1

EC 2.7.11.34

XP_047278610.1

EC 2.7.11.34

XP_054217767.1

EC 2.7.11.34

XP_054217768.1

EC 2.7.11.34

XP_054217769.1

EC 2.7.11.34

XP_054217770.1

EC 2.7.11.34

XP_054217771.1

EC 2.7.11.34

XP_054217772.1

EC 2.7.11.34

XP_054217773.1

EC 2.7.11.34

XP_054217774.1

EC 2.7.11.34

XP_054217775.1

EC 2.7.11.34

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001145001.3 → NP_001138473.1 serine/threonine-protein kinase Nek6 isoform 1

See identical proteins and their annotated locations for NP_001138473.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 7 encode the same isoform (1).

Source sequence(s)

AF087909, AL137846, BC004174, BC012761, CF994196

Consensus CDS

CCDS48015.1

UniProtKB/Swiss-Prot

Q9HC98

Related

ENSP00000362702.3, ENST00000373600.7

Conserved Domains (2) summary

smart00220
Location:79 → 344

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08228
Location:76 → 343

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
NM_001166167.2 → NP_001159639.1 serine/threonine-protein kinase Nek6 isoform 3

See identical proteins and their annotated locations for NP_001159639.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AF087909, AL137846, BC012761, BM083872

Consensus CDS

CCDS55338.1

UniProtKB/Swiss-Prot

Q9HC98

Related

ENSP00000441469.1, ENST00000540326.5

Conserved Domains (2) summary

smart00220
Location:63 → 328

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08228
Location:60 → 327

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
NM_001166168.2 → NP_001159640.1 serine/threonine-protein kinase Nek6 isoform 2

See identical proteins and their annotated locations for NP_001159640.1

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 5 and 6 encode the same isoform (2).

Source sequence(s)

AF087909, AL137846, AL162724, BC012761, BP279761

Consensus CDS

CCDS6854.1

UniProtKB/Swiss-Prot

B7Z2D9, Q5TBG3, Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9HC98, Q9ULX2

Related

ENSP00000442636.1, ENST00000545174.5

Conserved Domains (1) summary

cd08228
Location:42 → 309

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
NM_001166169.2 → NP_001159641.1 serine/threonine-protein kinase Nek6 isoform 4

See identical proteins and their annotated locations for NP_001159641.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AK294614, AL137846, AL162724, BC012761

Consensus CDS

CCDS55339.1

UniProtKB/Swiss-Prot

Q9HC98

Related

ENSP00000439651.1, ENST00000539416.5

Conserved Domains (2) summary

smart00220
Location:70 → 335

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08228
Location:67 → 334

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
NM_001166170.2 → NP_001159642.1 serine/threonine-protein kinase Nek6 isoform 2

See identical proteins and their annotated locations for NP_001159642.1

Status: REVIEWED

Description

Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 5 and 6 encode the same isoform (2).

Source sequence(s)

AL137846, AL162724, BC012761

Consensus CDS

CCDS6854.1

UniProtKB/Swiss-Prot

B7Z2D9, Q5TBG3, Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9HC98, Q9ULX2

Related

ENSP00000441426.1, ENST00000546191.5

Conserved Domains (1) summary

cd08228
Location:42 → 309

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
NM_001166171.2 → NP_001159643.1 serine/threonine-protein kinase Nek6 isoform 1

See identical proteins and their annotated locations for NP_001159643.1

Status: REVIEWED

Description

Transcript Variant: This variant (7) differs in the 5' UTR compared to variant 1. Both variants 1 and 7 encode the same protein.

Source sequence(s)

AL137846, AL162724, BC012761, DB350175

Consensus CDS

CCDS48015.1

UniProtKB/Swiss-Prot

Q9HC98

Related

ENSP00000377749.2, ENST00000394199.6

Conserved Domains (2) summary

smart00220
Location:79 → 344

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08228
Location:76 → 343

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
NM_014397.6 → NP_055212.2 serine/threonine-protein kinase Nek6 isoform 2

See identical proteins and their annotated locations for NP_055212.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 5 and 6 encode the same isoform (2).

Source sequence(s)

AF087909, AL137846, BC012761, CF994196

Consensus CDS

CCDS6854.1

UniProtKB/Swiss-Prot

B7Z2D9, Q5TBG3, Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9HC98, Q9ULX2

Related

ENSP00000319734.5, ENST00000320246.10

Conserved Domains (1) summary

cd08228
Location:42 → 309

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000009.12 Reference GRCh38.p14 Primary Assembly

Range

124257606..124353307

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047422652.1 → XP_047278608.1 serine/threonine-protein kinase Nek6 isoform X2
XM_024447387.2 → XP_024303155.1 serine/threonine-protein kinase Nek6 isoform X4

UniProtKB/Swiss-Prot

B7Z2D9, Q5TBG3, Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9HC98, Q9ULX2

Conserved Domains (1) summary

cd08228
Location:42 → 309

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
XM_047422653.1 → XP_047278609.1 serine/threonine-protein kinase Nek6 isoform X3
XM_017014217.2 → XP_016869706.1 serine/threonine-protein kinase Nek6 isoform X2

UniProtKB/Swiss-Prot

Q9HC98

Conserved Domains (2) summary

smart00220
Location:79 → 344

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08228
Location:76 → 343

STKc_Nek6; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 6
XM_047422651.1 → XP_047278607.1 serine/threonine-protein kinase Nek6 isoform X1
XM_047422654.1 → XP_047278610.1 serine/threonine-protein kinase Nek6 isoform X2