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    Mest mesoderm specific transcript [ Mus musculus (house mouse) ]

    Gene ID: 17294, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Mestprovided by MGI
    Official Full Name
    mesoderm specific transcriptprovided by MGI
    Primary source
    MGI:MGI:96968
    See related
    Ensembl:ENSMUSG00000051855 AllianceGenome:MGI:96968
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Peg1
    Summary
    Predicted to enable hydrolase activity. Involved in regulation of lipid storage and response to retinoic acid. Located in endoplasmic reticulum. Is expressed in several structures, including central nervous system; embryo mesenchyme; extraembryonic component; heart and pericardium; and neural retina. Used to study Barth syndrome. Orthologous to human MEST (mesoderm specific transcript). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in limb E14.5 (RPKM 597.0), CNS E11.5 (RPKM 207.7) and 9 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Mest in Genome Data Viewer
    Location:
    6 A3.3; 6 12.53 cM
    Exon count:
    15
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (30733505..30783580)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (30733506..30748466)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene carboxypeptidase A1, pancreatic Neighboring gene centrosomal protein 41 Neighboring gene predicted gene 13782 Neighboring gene STARR-positive B cell enhancer mm9_chr6:30643637-30643938 Neighboring gene STARR-seq mESC enhancer starr_15474 Neighboring gene microRNA 335 Neighboring gene coatomer protein complex, subunit gamma 2 Neighboring gene coatomer protein complex, subunit gamma 2, opposite strand 2 Neighboring gene RIKEN cDNA 4930412F09 gene Neighboring gene predicted gene, 38781 Neighboring gene testis specific gene A13

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Peg1/Mest, an imprinted gene, is involved in mammary gland maturation. Yonekura S, et al. J Cell Physiol, 2019 Feb. PMID 30144363
    2. Identification of developmentally regulated mesodermal-specific transcript in mouse embryonic metanephros. Kanwar YS, et al. Am J Physiol Renal Physiol, 2002 May. PMID 11934706
    3. Dynamic CpG and non-CpG methylation of the Peg1/Mest gene in the mouse oocyte and preimplantation embryo. Imamura T, et al. J Biol Chem, 2005 May 20. PMID 15778220
    4. Diet-induced adipose tissue expansion is mitigated in mice with a targeted inactivation of mesoderm specific transcript (Mest). Anunciado-Koza RP, et al. PLoS One, 2017. PMID 28640866, Free PMC Article
    5. Gene expression of mesoderm-specific transcript is upregulated as preadipocytes differentiate to adipocytes in vitro. Kadota Y, et al. J Physiol Sci, 2012 Sep. PMID 22753118, Free PMC Article

    See all (132) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The paternally imprinted gene Peg1/Mest is involved in mammary alveoli formation.
      Title: Peg1/Mest, an imprinted gene, is involved in mammary gland maturation.
    2. Identification of MEST as an endoplasmic reticulum-specific protein that co-localizes with lipid droplets in cells undergoing adipogenic differentiation supports a function for MEST in the facilitation of lipid accumulation and storage in adipocytes.
      Title: Mesoderm-specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy.
    3. This study demonstrated that the Mest and its intron product, miR-335, play important roles in neuronal migration with Mest regulating the morphological transition of primary neurons required in the formation of the mammalian neocortex.
      Title: Defective neuronal migration and inhibition of bipolar to multipolar transition of migrating neural cells by Mesoderm-Specific Transcript, Mest, in the developing mouse neocortex.
    4. diet-induced ATE in MEST-deficient mice diminishes hypoxia and inflammation in WAT leading to improved glucose tolerance and insulin sensitivity
      Title: Diet-induced adipose tissue expansion is mitigated in mice with a targeted inactivation of mesoderm specific transcript (Mest).
    5. The androgenetic patterns of H19, Snrpn, and Mest were maintained even after differentiation of germline-derived pluripotent stem cells(gPS) into neural stem cells(NSC), whereas the fully unmethylated status of Ndn in SSCs was altered to somatic patterns in gPS cells and gPS-NSCs.
      Title: Epigenetic alteration of imprinted genes during neural differentiation of germline-derived pluripotent stem cells.
    6. The balance of factors controlling fat deposition can be evaluated in part by the differential expression profiles of Mest and Sfrp5 genes with functions linked to fat deposition as long as there is an active accumulation of fat mass.
      Title: Mest and Sfrp5 are biomarkers for healthy adipose tissue.
    7. the expression of Mest and Sfrp5 were tightly associated across the 5 mouse strains with the highest and lowest expression occurring in DBA/2J and C57BL/6J (B6) respectively suggesting a common mechanism for their regulation.
      Title: Adipose tissue Mest and Sfrp5 are concomitant with variations of adiposity among inbred mouse strains fed a non-obesogenic diet.
    8. Although both Mest and miR-335 are highly expressed during muscle development and regeneration, only Mest+/- (maternal/paternal) mice show retardation of body growth.
      Title: Mest but Not MiR-335 Affects Skeletal Muscle Growth and Regeneration.
    9. The paternally expressed imprinted gene Mest was only detected in haploid androgenetic blastocysts and was significantly lower in this group relative to the controls, indicating that the gene maintains expression patterns specific to its parental origin.
      Title: Analysis of imprinted gene expression and implantation in haploid androgenetic mouse embryos.
    10. analysis of modifications of H3 histone in H19 and MEST imprinted genes in blastocysts produced in vitro from non-vitrified and vitrified two-cell embryos
      Title: H19 and MEST gene expression and histone modification in blastocysts cultured from vitrified and fresh two-cell mouse embryos.
    Submit: New GeneRIF Correction See all GeneRIFs (24)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Targeted (3)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in regulation of lipid storage IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to retinoic acid IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mesoderm-specific transcript protein
    Names
    paternally-expressed gene 1 protein

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001252292.1 → NP_001239221.1  mesoderm-specific transcript protein isoform 1

      See identical proteins and their annotated locations for NP_001239221.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).
      Source sequence(s)
      AK146457, AK168732, AK169266, BF608470
      Consensus CDS
      CCDS80512.1
      UniProtKB/TrEMBL
      D3Z383
      Related
      ENSMUSP00000117713.3, ENSMUST00000124665.3
      Conserved Domains (1) summary
      COG0596
      Location:54 → 328
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

    2. NM_001252293.1 → NP_001239222.1  mesoderm-specific transcript protein isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) has a different 5' end, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus when it is compared to isoform 1.
      Source sequence(s)
      AF482999, AK146457, BF608470, CF916557
      Consensus CDS
      CCDS80513.1
      UniProtKB/Swiss-Prot
      Q07646
      Related
      ENSMUSP00000119038.3, ENSMUST00000157040.8
      Conserved Domains (1) summary
      COG0596
      Location:38 → 312
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

    3. NM_008590.2 → NP_032616.1  mesoderm-specific transcript protein isoform 2

      See identical proteins and their annotated locations for NP_032616.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.
      Source sequence(s)
      AK032881, BF608470, CJ162886
      Consensus CDS
      CCDS19979.1
      UniProtKB/Swiss-Prot
      Q07646, Q792T8, Q8BS88, Q8K463, Q99KT5
      Related
      ENSMUSP00000129639.3, ENSMUST00000163949.9
      Conserved Domains (1) summary
      COG0596
      Location:47 → 321
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      30733505..30783580
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036165900.1 → XP_036021793.1  mesoderm-specific transcript protein isoform X2

      UniProtKB/Swiss-Prot
      Q07646, Q792T8, Q8BS88, Q8K463, Q99KT5
      Conserved Domains (1) summary
      COG0596
      Location:47 → 321
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

    2. XM_036165902.1 → XP_036021795.1  mesoderm-specific transcript protein isoform X4

      Conserved Domains (1) summary
      COG0596
      Location:47 → 295
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

    3. XM_036165901.1 → XP_036021794.1  mesoderm-specific transcript protein isoform X3

      Conserved Domains (1) summary
      COG0596
      Location:54 → 302
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

    4. XM_036165899.1 → XP_036021792.1  mesoderm-specific transcript protein isoform X1

      UniProtKB/TrEMBL
      D3Z383
      Conserved Domains (1) summary
      COG0596
      Location:54 → 328
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q07646.1 GenPept UniProtKB/Swiss-Prot:Q07646

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