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    CPK28 calcium-dependent protein kinase 28 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 836753, updated on 18-Sep-2024

    Summary

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    Official Symbol
    CPK28
    Official Full Name
    calcium-dependent protein kinase 28
    Primary source
    TAIR:AT5G66210
    Locus tag
    AT5G66210
    See related
    Araport:AT5G66210
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    calcium-dependent protein kinase 28; K2A18.29; K2A18_29
    Summary
    member of Calcium Dependent Protein Kinase
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    Genomic context

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    See CPK28 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    11
    Sequence:
    Chromosome: 5; NC_003076.8 (26456142..26459654, complement)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene sulfurtransferase 18 Neighboring gene S-adenosyl-L-methionine-dependent methyltransferases superfamily protein Neighboring gene ferredoxin-NADP[+]-oxidoreductase 1 Neighboring gene armadillo repeat only 2 Neighboring gene Chalcone-flavanone isomerase family protein Neighboring gene Chalcone-flavanone isomerase family protein Neighboring gene miscRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. CPK28 is a modulator of purinergic signaling in plant growth and defense. Sowders JM, et al. Plant J, 2024 May. PMID 38308597,
    2. Phosphorylation status of CPK28 affects its ubiquitination and protein stability. Liu X, et al. New Phytol, 2023 Feb. PMID 36333900
    3. Phosphorylation-dependent subfunctionalization of the calcium-dependent protein kinase CPK28. Bredow M, et al. Proc Natl Acad Sci U S A, 2021 May 11. PMID 33941701, Free PMC Article
    4. Calcium-dependent protein kinase CPK28 targets the methionine adenosyltransferases for degradation by the 26S proteasome and affects ethylene biosynthesis and lignin deposition in Arabidopsis. Jin Y, et al. Plant J, 2017 Apr. PMID 28112445
    5. The calcium-dependent protein kinase CPK28 regulates development by inducing growth phase-specific, spatially restricted alterations in jasmonic acid levels independent of defense responses in Arabidopsis. Matschi S, et al. Plant Cell, 2015 Mar. PMID 25736059, Free PMC Article

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. CPK28 is a modulator of purinergic signaling in plant growth and defense.
      Title: CPK28 is a modulator of purinergic signaling in plant growth and defense.
    2. Phosphorylation status of CPK28 affects its ubiquitination and protein stability.
      Title: Phosphorylation status of CPK28 affects its ubiquitination and protein stability.
    3. The calcium-dependent protein kinase CPK28 is targeted by the ubiquitin ligases ATL31 and ATL6 for proteasome-mediated degradation to fine-tune immune signaling in Arabidopsis.
      Title: The calcium-dependent protein kinase CPK28 is targeted by the ubiquitin ligases ATL31 and ATL6 for proteasome-mediated degradation to fine-tune immune signaling in Arabidopsis.
    4. Phosphorylation-dependent subfunctionalization of the calcium-dependent protein kinase CPK28.
      Title: Phosphorylation-dependent subfunctionalization of the calcium-dependent protein kinase CPK28.
    5. The work suggests that CPK28 targets MATs (MAT1, MAT2 and MAT3) for degradation by the 26S proteasome pathway, and thus affects ethylene biosynthesis and lignin deposition in Arabidopsis.
      Title: Calcium-dependent protein kinase CPK28 targets the methionine adenosyltransferases for degradation by the 26S proteasome and affects ethylene biosynthesis and lignin deposition in Arabidopsis.
    6. CPK28 confers developmental processes by the tissue-specific balance of jasmonic acid (JA) and gibberellic acid without affecting JA-mediated defense responses.
      Title: The calcium-dependent protein kinase CPK28 regulates development by inducing growth phase-specific, spatially restricted alterations in jasmonic acid levels independent of defense responses in Arabidopsis.
    7. contributes to the PAMP-induced Ca(2+) burst, supporting its role as a negative regulator of BIK1
      Title: The calcium-dependent protein kinase CPK28 negatively regulates the BIK1-mediated PAMP-induced calcium burst.
    8. CPK28 facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several pathogen-associated molecular patterns
      Title: The calcium-dependent protein kinase CPK28 buffers plant immunity and regulates BIK1 turnover.
    9. CPK28 is a developmentally controlled regulator for coordinated stem elongation and secondary growth.
      Title: Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    NM_001085337.1
    NM_001126036.2
    NM_001345751.1
    NM_001345752.1
    NM_126019.4
    NM_180949.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables calcium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein serine/threonine phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in negative regulation of defense response to bacterium IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of secondary shoot formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    calcium-dependent protein kinase 28
    NP_001078806.1
    • calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, ATP binding, calcium ion binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 122907 Blast hits to 120110 proteins in 3700 species: Archae - 157; Bacteria - 14345; Metazoa - 44968; Fungi - 14295; Plants - 27583; Viruses - 455; Other Eukaryotes - 21104 (source: NCBI BLink).
    NP_001119508.1
    • calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, calcium ion binding, ATP binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), EF-hand-like domain (InterPro:IPR011992), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    NP_201422.1
    • calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, ATP binding, calcium ion binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    NP_851280.1
    • calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, calcium ion binding, ATP binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 124563 Blast hits to 121753 proteins in 3731 species: Archae - 157; Bacteria - 14240; Metazoa - 45960; Fungi - 14836; Plants - 27830; Viruses - 455; Other Eukaryotes - 21085 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      26456142..26459654 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_180949.3 → NP_851280.1  calcium-dependent protein kinase 28 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_851280.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A8MQP5, Q0WNW9, Q8LDS1, Q9FKW4
      UniProtKB/TrEMBL
      A0A178UH42, A0A5S9YI95, A0A7G2FJB0
      Conserved Domains (2) summary
      COG5126
      Location:346 → 502
      FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]
      cd05117
      Location:61 → 321
      STKc_CAMK; The catalytic domain of CAMK family Serine/Threonine Kinases

    2. NM_001345751.1 → NP_001331199.1  calcium-dependent protein kinase 28 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1P8BD52, A0A7G2FJB0
      Conserved Domains (2) summary
      COG5126
      Location:346 → 472
      FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]
      cd05117
      Location:61 → 321
      STKc_CAMK; The catalytic domain of CAMK family Serine/Threonine Kinases

    3. NM_001126036.2 → NP_001119508.1  calcium-dependent protein kinase 28 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001119508.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A7G2FJB0
      Conserved Domains (2) summary
      smart00220
      Location:62 → 322
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd05117
      Location:61 → 321
      STKc_CAMK; The catalytic domain of CAMK family Serine/Threonine Kinases

    4. NM_001345752.1 → NP_001331200.1  calcium-dependent protein kinase 28 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A7G2FJB0

    5. NM_126019.4 → NP_201422.1  calcium-dependent protein kinase 28 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_201422.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A8MQP5, Q0WNW9, Q8LDS1, Q9FKW4
      UniProtKB/TrEMBL
      A0A178UH42, A0A5S9YI95, A0A7G2FJB0
      Conserved Domains (2) summary
      COG5126
      Location:346 → 502
      FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]
      cd05117
      Location:61 → 321
      STKc_CAMK; The catalytic domain of CAMK family Serine/Threonine Kinases

    6. NM_001085337.1 → NP_001078806.1  calcium-dependent protein kinase 28 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001078806.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A7G2FJB0, B9DGD0
      Conserved Domains (4) summary
      smart00220
      Location:62 → 322
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd00051
      Location:369 → 430
      EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
      cd05117
      Location:61 → 321
      STKc_CAMK; The catalytic domain of CAMK family Serine/Threonine Kinases
      pfam13499
      Location:370 → 430
      EF-hand_7; EF-hand domain pair
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9FKW4.1

    Gene LinkOut

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