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    ATSPO11-1 Spo11/DNA topoisomerase VI, subunit A protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 820506, updated on 18-Sep-2024

    Summary

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    Official Symbol
    ATSPO11-1
    Official Full Name
    Spo11/DNA topoisomerase VI, subunit A protein
    Primary source
    TAIR:AT3G13170
    Locus tag
    AT3G13170
    See related
    Araport:AT3G13170
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    SPO11-1
    Summary
    Encodes AtSPO11-1, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. AtSPO11-1 accumulates in foci in early G2. At 1 h post-S phase, no foci are observed, but by 3 h a majority (80%) of meiocytes at this time point contain >50 foci. However, by 5 h, AtSPO11-1 foci are no longer detectable. This suggests that the protein undergoes a rapid cycle of accumulation and disappearance in meiocytes over a period of between 1 and 5 h post-S phase.
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    Genomic context

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    See ATSPO11-1 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    15
    Sequence:
    Chromosome: 3; NC_003074.8 (4231395..4234206, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene hydroxyproline-rich glycoprotein family protein Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene uncharacterized protein Neighboring gene NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana. Chen HW, et al. Nucleic Acids Res, 2024 May 8. PMID 38499490, Free PMC Article
    2. Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions. Choi K, et al. Genome Res, 2018 Apr. PMID 29530928, Free PMC Article
    3. A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis. Shingu Y, et al. FEBS J, 2010 May. PMID 20423461
    4. AtSPO11-1 is necessary for efficient meiotic recombination in plants. Grelon M, et al. EMBO J, 2001 Feb 1. PMID 11157765, Free PMC Article
    5. Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern. Sprink T, et al. Int J Mol Sci, 2021 Aug 28. PMID 34502253, Free PMC Article

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.
      Title: Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.
    2. Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.
      Title: Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.
    3. As transposon mobility in plant genomes is restricted by DNA methylation, we used the met1 DNA methyltransferase mutant to investigate the role of heterochromatin in SPO11-1-oligo distributions. Epigenetic activation of meiotic DSBs in proximity to centromeres and transposons occurred in met1 mutants, coincident with reduced nucleosome occupancy, gain of transcription, and H3K4me3
      Title: Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions.
    4. Results identified a group of spo11-1 mutants with defects in meiosis that specifically correlate with the defects in DNA binding at an identified DNA binding surface on SPO11-1.
      Title: A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis.
    5. Data show that the catalytically active tyrosine residues of SPO11-1 are required for meiotic double-strand break induction in Arabidopsis.
      Title: The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis.
    6. AtSPO11-1 and AtSPO11-2 genetically interact and function in meiotic recombination. [AtSPO11-1]
      Title: Arabidopsis SPO11-2 functions with SPO11-1 in meiotic recombination.
    Submit: New GeneRIF Correction

    General gene information

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    NM_001338034.1
    NM_001338035.1
    NM_112156.2

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within chiasma assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within homologous chromosome pairing at meiosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within maintenance of meiotic sister chromatid cohesion IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within meiotic DNA double-strand break formation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within meiotic DNA double-strand break processing IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within reciprocal meiotic recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    Spo11/DNA topoisomerase VI, subunit A protein
    NP_187923.1
    • ATSPO11-1; CONTAINS InterPro DOMAIN/s: Spo11/DNA topoisomerase VI, subunit A, N-terminal (InterPro:IPR013049), Meiotic recombination, Spo11 (InterPro:IPR013048), Spo11/DNA topoisomerase VI, subunit A (InterPro:IPR002815); BEST Arabidopsis thaliana protein match is: Spo11/DNA topoisomerase VI, subunit A protein (TAIR:AT5G02820.1); Has 908 Blast hits to 908 proteins in 313 species: Archae - 216; Bacteria - 16; Metazoa - 170; Fungi - 122; Plants - 151; Viruses - 0; Other Eukaryotes - 233 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      4231395..4234206 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_112156.2 → NP_187923.1  Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_187923.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9C5S3, Q9LK56, Q9M4A2
      UniProtKB/TrEMBL
      A0A178VGV5, A0A5S9XC69, A0A654FGW4
      Conserved Domains (1) summary
      COG1697
      Location:6 → 357
      Spo11; DNA topoisomerase VI, subunit A [Replication, recombination and repair]

    2. NM_001338034.1 → NP_001326966.1  Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1I9LQV8

    3. NM_001338035.1 → NP_001326967.1  Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1I9LQV8
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9M4A2.1

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