ABCB4 ATP binding cassette subfamily B4 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: ABCB4
Official Full Name: ATP binding cassette subfamily B4
Primary source: TAIR:AT2G47000
Locus tag: AT2G47000
See related: Araport:AT2G47000
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS P-GLYCOPROTEIN 4; AtABCB4; ATP binding cassette subfamily B4; ATP-binding cassette B4; ATPGP4; F14M4.17; MDR4; MULTIDRUG RESISTANCE 4; P-GLYCOPROTEIN 4; PGP4
Summary: Encodes an auxin efflux transmembrane transporter that is a member of the multidrug resistance P-glycoprotein (MDR/PGP) subfamily of ABC transporters. Functions in the basipetal redirection of auxin from the root tip. Exhibits apolar plasma membrane localization in the root cap and polar localization in tissues above and is involved in root hair elongation.
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Genomic context

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See ABCB4 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 12

Sequence:: Chromosome: 2; NC_003071.7 (19309643..19315343, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

2,4-dichlorophenoxyacetic acid (2,4-D) is a non-competitive inhibitor of indole-3-acetic acid (IAA) transport by ABCB4 and indicate that ABCB4 is a target of 2,4-D herbicidal activity.
Title: The Arabidopsis concentration-dependent influx/efflux transporter ABCB4 regulates cellular auxin levels in the root epidermis.
ABCB4 localizes in a nonpolar manner and is stably retained in the plasma membrane.
Title: ATP-binding cassette B4, an auxin-efflux transporter, stably associates with the plasma membrane and shows distinctive intracellular trafficking from that of PIN-FORMED proteins.
Results suggest that PGP4 is an auxin efflux transporter in plants and that its trafficking to the plasma membrane involves both BFA-sensitive and -insensitive pathways.
Title: P-glycoprotein4 displays auxin efflux transporter-like action in Arabidopsis root hair cells and tobacco cells.
Mutations in Multidrug Resistance-Like1 (MDR1) reduced acropetal auxin transport in roots by 80% without affecting basipetal transport. Conversely, mutations in MDR4 blocked 50% of basipetal transport without affecting acropetal transport.
Title: Separating the roles of acropetal and basipetal auxin transport on gravitropism with mutations in two Arabidopsis multidrug resistance-like ABC transporter genes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001337233.1

Supported by ESTs:

NM_001337234.1

Supported by ESTs:

NM_001337235.1

Supported by ESTs:

NM_001337236.1

Supported by ESTs:

NM_001337237.1

Supported by ESTs:

NM_001337238.1

Supported by ESTs:

NM_130268.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ABC-type transporter activity	IEA Inferred from Electronic Annotation more info
enables ABC-type xenobiotic transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables auxin efflux transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables auxin influx transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within auxin export across the plasma membrane	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within auxin export across the plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within auxin import into cell	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within auxin polar transport	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within basipetal auxin transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within gravitropism	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to auxin	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to cytokinin	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within root hair elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
located_in membrane	HDA more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: ATP binding cassette subfamily B4

NP_182223.1

ATP binding cassette subfamily B4 (ABCB4); FUNCTIONS IN: xenobiotic-transporting ATPase activity, ATPase activity, coupled to transmembrane movement of substances; INVOLVED IN: in 8 processes; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ABC transporter-like (InterPro:IPR003439), ABC transporter, transmembrane domain, type 1 (InterPro:IPR011527), ABC transporter integral membrane type 1 (InterPro:IPR017940), ABC transporter, transmembrane domain (InterPro:IPR001140), ABC transporter, conserved site (InterPro:IPR017871); BEST Arabidopsis thaliana protein match is: P-glycoprotein 21 (TAIR:AT3G62150.1); Has 844144 Blast hits to 390751 proteins in 4168 species: Archae - 14748; Bacteria - 659498; Metazoa - 17847; Fungi - 13026; Plants - 10001; Viruses - 45; Other Eukaryotes - 128979 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

19309643..19315343 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_130268.4 → NP_182223.1 ATP binding cassette subfamily B4 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_182223.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O80725

UniProtKB/TrEMBL

A0A178VT74, A0A5S9X7N0

Conserved Domains (1) summary

PTZ00265
Location:46 → 1280

PTZ00265; multidrug resistance protein (mdr1); Provisional
NM_001337233.1 → NP_001323915.1 ATP binding cassette subfamily B4 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AYL3, A0A5S9X7N0
NM_001337235.1 → NP_001323913.1 ATP binding cassette subfamily B4 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AYF4, A0A5S9X7N0

Conserved Domains (4) summary

cd03249
Location:384 → 622

ABC_MTABC3_MDL1_MDL2; ATP-binding cassette domain of a mitochondrial protein MTABC3 and related proteins

COG1132
Location:45 → 625

MdlB; ABC-type multidrug transport system, ATPase and permease component [Defense mechanisms]

cd18578
Location:710 → 1025

ABC_6TM_Pgp_ABCB1_D2_like; Six-transmembrane helical domain 2 (TMD2) of P-glycoprotein 1 (Pgp) and related proteins

cl38936
Location:1042 → 1071

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001337234.1 → NP_001323916.1 ATP binding cassette subfamily B4 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AYG2

Conserved Domains (3) summary

cd03249
Location:384 → 622

ABC_MTABC3_MDL1_MDL2; ATP-binding cassette domain of a mitochondrial protein MTABC3 and related proteins

COG1132
Location:45 → 625

MdlB; ABC-type multidrug transport system, ATPase and permease component [Defense mechanisms]

cd18578
Location:710 → 984

ABC_6TM_Pgp_ABCB1_D2_like; Six-transmembrane helical domain 2 (TMD2) of P-glycoprotein 1 (Pgp) and related proteins
NM_001337236.1 → NP_001323914.1 ATP binding cassette subfamily B4 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

O80725

UniProtKB/TrEMBL

A0A178VT74, A0A5S9X7N0

Conserved Domains (1) summary

PTZ00265
Location:46 → 1280

PTZ00265; multidrug resistance protein (mdr1); Provisional
NM_001337237.1 → NP_001323911.1 ATP binding cassette subfamily B4 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

O80725

UniProtKB/TrEMBL

A0A178VT74, A0A5S9X7N0

Conserved Domains (1) summary

PTZ00265
Location:46 → 1280

PTZ00265; multidrug resistance protein (mdr1); Provisional
NM_001337238.1 → NP_001323912.1 ATP binding cassette subfamily B4 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AYG2

Conserved Domains (3) summary

cd03249
Location:384 → 622

ABC_MTABC3_MDL1_MDL2; ATP-binding cassette domain of a mitochondrial protein MTABC3 and related proteins

COG1132
Location:45 → 625

MdlB; ABC-type multidrug transport system, ATPase and permease component [Defense mechanisms]

cd18578
Location:710 → 984

ABC_6TM_Pgp_ABCB1_D2_like; Six-transmembrane helical domain 2 (TMD2) of P-glycoprotein 1 (Pgp) and related proteins