MYB12 myb domain protein 12 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: MYB12
Official Full Name: myb domain protein 12
Primary source: TAIR:AT2G47460
Locus tag: AT2G47460
See related: Araport:AT2G47460
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATMYB12; myb domain protein 12; MYB DOMAIN PROTEIN 12; PFG1; PRODUCTION OF FLAVONOL GLYCOSIDES 1; T30B22.24
Summary: ''MYB12 belongs to subgroup 7 of the R2R3-MYB family. It strongly activates the promoters of chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and - to a lesser extent - chalcone flavanone isomerase (CHI), but cannot activate the promoters of flavonoid-3'hydroxylase (F3'H) and dihydroflavonol 4-reductase (DF). The activation requires a functional MYB recognition element (MRE). Results from the myb12-1f allele indicate that an activation domain might be present in the C-terminus. Overexpression or knock-out plants do not show any obvious phenotype under greenhouse conditions. Young myb12-ko seedlings contain reduced amounts of flavonoids (quercetin and kaempferol), while seedlings as well as leaves of MYB12-OX plants displayed an increased flavonoid content. They did not show any significant difference in anthocyanin content. Expression of CHS and FLS shows a clear correlation to MYB12 expression levels. CHI and F3H show increased transcript levels in the MYB12-OX lines, but no differences in the knock-out. Even in the absence of functional MYB12, flavonol biosynthesis is not completely absent, suggesting functional redundancy. '' The redundant factors are MYB11 and MYB111 although MYB12 is primarily required for flavonol biosynthesis in roots. Mutations in MYB12 block both auxin and ethylene stimulation of flavonoid synthesis.
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Genomic context

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See MYB12 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 3

Sequence:: Chromosome: 2; NC_003071.7 (19476326..19479597)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The transcription factor AtMYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature.
Title: The transcription factor AtMYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature.
The R2R3-MYB transcription factor MYB12 (AtMYB12) activation domain (AD) was identified in the amino acid region 273 to 325 (of 371), with the region 309-325 having the most relevant effect on activation capacity.
Title: The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor.
Results showed that AtMYB12-overexpressing Arabidopsis plants exhibited increased levels of flavonoids and enhanced salt and drought tolerance suggesting that overexpression of AtMYB12 up-regulates genes involved in flavonoid biosynthesis, abscisic acid biosynthesis, proline biosynthesis and ROS-scavenging system, resulting in significant physiological changes.
Title: AtMYB12 regulates flavonoids accumulation and abiotic stress tolerance in transgenic Arabidopsis thaliana.
results demonstrate that modulation of primary carbon metabolism and other pathways by AtMYB12 in tomato may lead to sufficient substrate supply for enhanced content of phenolics in general and flavonols in particular.
Title: AtMYB12 expression in tomato leads to large scale differential modulation in transcriptome and flavonoid content in leaf and fruit tissues.
AtMYB12 was overexpressed in a commercial cultivar of kale and the transgenic plants were tested both in in vitro and in semi-field conditions. A severalfold increase in both total phenolics content and flavonol accumulation was achieved.
Title: Heterologous expression of AtMYB12 in kale (Brassica oleracea var. acephala) leads to high flavonol accumulation.
The quantity and diversity of flavonoids is increased by overexpressing MYB12/PAP1. Flavonoids enhances both biotic and abiotic stress tolerance in crops.
Title: Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids.
The characterization of MYB12 as a flavonol-specific activator of flavonoid biosynthesis in A. thaliana is reported.
Title: The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_130314.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription cis-regulatory region binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within flavonoid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in flavonol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of flavonol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to UV-B	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to UV-B	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to auxin	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to ethylene	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to light stimulus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to salt stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: myb domain protein 12

NP_182268.1

myb domain protein 12 (MYB12); CONTAINS InterPro DOMAIN/s: SANT, DNA-binding (InterPro:IPR001005), Homeodomain-like (InterPro:IPR009057), Myb, DNA-binding (InterPro:IPR014778), HTH transcriptional regulator, Myb-type, DNA-binding (InterPro:IPR017930), Homeodomain-related (InterPro:IPR012287), Myb transcription factor (InterPro:IPR015495); BEST Arabidopsis thaliana protein match is: myb domain protein 11 (TAIR:AT3G62610.1); Has 8833 Blast hits to 8133 proteins in 470 species: Archae - 0; Bacteria - 0; Metazoa - 786; Fungi - 423; Plants - 5965; Viruses - 6; Other Eukaryotes - 1653 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

19476326..19479597

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_130314.4 → NP_182268.1 myb domain protein 12 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_182268.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O22264, Q38IY2, Q9SBG6

UniProtKB/TrEMBL

A0A5S9X8B7, A0A654F7Y3

Conserved Domains (1) summary

PLN03212
Location:5 → 116

PLN03212; Transcription repressor MYB5; Provisional