eri-1 3'-5' exonuclease eri-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: eri-1
Official Full Name: 3'-5' exonuclease eri-1
Primary source: WormBase:WBGene00001332
Locus tag: CELE_T07A9.5
See related: AllianceGenome:WB:WBGene00001332
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables 3'-5'-RNA exonuclease activity. Involved in several processes, including RNA metabolic process; meiotic chromosome segregation; and regulation of gene expression. Located in cytoplasm. Is expressed in neurons and spermatheca. Orthologous to human ERI1 (exoribonuclease 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See eri-1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 12

Sequence:: Chromosome: IV; NC_003282.8 (415635..419958, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In the absence of eri-1, reserpine mediated lifespan extension (RMLE) is shortened. In the dop-3 loss-of-function background, lack of eri-1 completely abolishes RMLE. This suggests that dop-3 and eri-1 act in independent parallel pathways for RMLE and these two pathways are essential and sufficient for the longevity enhancement by reserpine in C. elegans.
Title: Reserpine requires the D2-type receptor, dop-3, and the exoribonuclease, eri-1, to extend the lifespan in C. elegans.
results identify novel components of the endo RNAi machinery, demonstrate differential requirements for the Eri factors in the sperm-producing germline, and begin to delineate the functional requirement for the ERI/DICER complex in sperm development
Title: Requirement for the ERI/DICER complex in endogenous RNA interference and sperm development in Caenorhabditis elegans.
In C. elegans, two protein isoforms of ERI-1 are localized to the cytoplasm, and each has distinct functions in ribosomal RNA processing and negative regulation of RNA interference.
Title: The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi.
These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes.
Title: Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants.
After exposure to dsRNA or siRNAs, animals with eri-1 mutations accumulate more siRNAs than wild-type animals; a negative regulator that may normally function to limit the duration, cell-type specificity or endogenous functions of RNA interference
Title: A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans.
Feeding unc-13 & eri-1 strains dsRNA corresponding to a neuronally expressed GFP reporter resulted in reduction of GFP in double mutants compared to GFP expression in eri-1 mutants, indicating that UNC-13 functions in conjunction with ERI-1 in RNAi.
Title: Synaptic neurotransmission protein UNC-13 affects RNA interference in neurons.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS4-100 (e-PCR)
- UniSTS:242087

T07A9.5 (e-PCR)
- UniSTS:313287

T07A9.4 (e-PCR)
- UniSTS:318445

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables 3'-5'-RNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5'-RNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IBA Inferred from Biological aspect of Ancestor more info
involved_in exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IDA Inferred from Direct Assay more info	PubMed
involved_in germ cell development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in multicellular organismal reproductive process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of post-transcriptional gene silencing by regulatory ncRNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated gene silencing	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 3'-5' exonuclease eri-1

NP_001368343.1

Confirmed by transcript evidence

NP_001368344.1

Confirmed by transcript evidence

NP_741292.1

Confirmed by transcript evidence

NP_741293.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

415635..419958 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_171244.5 → NP_741292.1 3'-5' exonuclease eri-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_741292.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O44406, O44407, U4PC85, U4PCK7

Conserved Domains (2) summary

cd06133
Location:151 → 334

ERI-1_3'hExo_like; DEDDh 3'-5' exonuclease domain of Caenorhabditis elegans ERI-1, human 3' exonuclease, and similar proteins

smart00513
Location:92 → 126

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation
NM_001380006.1 → NP_001368344.1 3'-5' exonuclease eri-1 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (1) summary

cd06133
Location:2 → 128

ERI-1_3'hExo_like; DEDDh 3'-5' exonuclease domain of Caenorhabditis elegans ERI-1, human 3' exonuclease, and similar proteins
NM_171245.5 → NP_741293.1 3'-5' exonuclease eri-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_741293.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O44406

Conserved Domains (2) summary

cd06133
Location:151 → 334

ERI-1_3'hExo_like; DEDDh 3'-5' exonuclease domain of Caenorhabditis elegans ERI-1, human 3' exonuclease, and similar proteins

smart00513
Location:92 → 126

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation
NM_001380007.1 → NP_001368343.1 3'-5' exonuclease eri-1 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (1) summary

cd06133
Location:2 → 128

ERI-1_3'hExo_like; DEDDh 3'-5' exonuclease domain of Caenorhabditis elegans ERI-1, human 3' exonuclease, and similar proteins