cnt-1 ANK_REP_REGION domain-containing protein;Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2;Truncated ctn-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: cnt-1
Official Full Name: ANK_REP_REGION domain-containing protein;Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2;Truncated ctn-1
Primary source: WormBase:WBGene00000565
Locus tag: CELE_Y17G7B.15
See related: AllianceGenome:WB:WBGene00000565
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables phosphatidylinositol phosphate binding activity and small GTPase binding activity. Involved in apoptotic process; negative regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; and positive regulation of endosome to plasma membrane protein transport. Located in basolateral plasma membrane; cytoplasmic side of apical plasma membrane; and endosome membrane. Used to study cancer. Orthologous to human ACAP1 (ArfGAP with coiled-coil, ankyrin repeat and PH domains 1); ACAP2 (ArfGAP with coiled-coil, ankyrin repeat and PH domains 2); and ACAP3 (ArfGAP with coiled-coil, ankyrin repeat and PH domains 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See cnt-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 13

Sequence:: Chromosome: II; NC_003280.10 (12072170..12088868, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The study reports the identification of a CED-3 caspase substrate in Caenorhabditis elegans, CNT-1, that is cleaved during apoptosis to generate an N-terminal phosphoinositide-binding fragment (tCNT-1).
Title: Caspase-activated phosphoinositide binding by CNT-1 promotes apoptosis by inhibiting the AKT pathway.
One RAB-10-binding partner that we identified, CNT-1, is the only C. elegans homolog of the mammalian Arf6 GTPase-activating proteins ACAP1 and ACAP2.
Title: RAB-10-GTPase-mediated regulation of endosomal phosphatidylinositol-4,5-bisphosphate.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y17G7B.16 (e-PCR)
- UniSTS:320385

Y17G7B.15 (e-PCR)
- UniSTS:319741

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-3,4,5-trisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in apoptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endosome to plasma membrane protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic side of apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in recycling endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in recycling endosome membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ANK_REP_REGION domain-containing protein;Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2;Truncated ctn-1

NP_001022413.1

Confirmed by transcript evidence

NP_001335541.1

Confirmed by transcript evidence

NP_001368630.1

Confirmed by transcript evidence

NP_001369802.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

12072170..12088868 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001383965.2 → NP_001369802.1 Truncated ctn-1 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9XXH8, Q9XXH9, Q9XZQ1, Q9XZQ2

Conserved Domains (6) summary

PTZ00322
Location:628 → 795

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

cd07603
Location:30 → 233

BAR_ACAPs; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with Coiled-coil, ANK repeat and PH domain containing proteins

cd13250
Location:278 → 377

PH_ACAP; ArfGAP with coiled-coil, ankyrin repeat and PH domains Pleckstrin homology (PH) domain

sd00045
Location:690 → 721

ANK; ANK repeat [structural motif]

cd08835
Location:447 → 565

ArfGap_ACAP; ArfGAP domain of ACAP (ArfGAP with Coiled-coil, ANK repeat and PH domains) proteins

pfam12796
Location:695 → 775

Ank_2; Ankyrin repeats (3 copies)
NM_001027242.4 → NP_001022413.1 Truncated ctn-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001022413.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9XXH8

Conserved Domains (6) summary

cd00204
Location:582 → 691

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd13250
Location:194 → 293

PH_ACAP; ArfGAP with coiled-coil, ankyrin repeat and PH domains Pleckstrin homology (PH) domain

pfam00169
Location:192 → 288

PH; PH domain

pfam01412
Location:365 → 481

ArfGap; Putative GTPase activating protein for Arf

pfam12796
Location:582 → 670

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:606 → 637

ANK; ANK repeat [structural motif]
NM_001381624.1 → NP_001368630.1 Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1N7SYT2

Conserved Domains (5) summary

PTZ00322
Location:377 → 544

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

cd13250
Location:27 → 126

PH_ACAP; ArfGAP with coiled-coil, ankyrin repeat and PH domains Pleckstrin homology (PH) domain

sd00045
Location:439 → 470

ANK; ANK repeat [structural motif]

cl28907
Location:196 → 314

ArfGap; GTPase-activating protein (GAP) for the ADP ribosylation factors (ARFs)

pfam12796
Location:444 → 524

Ank_2; Ankyrin repeats (3 copies)
NM_001348640.3 → NP_001335541.1 ANK_REP_REGION domain-containing protein [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1N7SYR6

Conserved Domains (4) summary

PTZ00322
Location:125 → 292

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

sd00045
Location:187 → 218

ANK; ANK repeat [structural motif]

cl28907
Location:1 → 62

ArfGap; GTPase-activating protein (GAP) for the ADP ribosylation factors (ARFs)

pfam12796
Location:192 → 272

Ank_2; Ankyrin repeats (3 copies)