unc-73 CRAL-TRIO domain-containing protein;DH domain-containing protein;Rho guanine nucleotide exchange factor 26;Triple functional domain protein [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: unc-73
Official Full Name: CRAL-TRIO domain-containing protein;DH domain-containing protein;Rho guanine nucleotide exchange factor 26;Triple functional domain protein
Primary source: WormBase:WBGene00006805
Locus tag: CELE_F55C7.7
See related: AllianceGenome:WB:WBGene00006805
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables guanyl-nucleotide exchange factor activity and small GTPase binding activity. Involved in several processes, including generation of neurons; modulation of chemical synaptic transmission; and regulation of neuron projection development. Located in axon; cytoplasm; and muscle cell projection. Is expressed in several structures, including egg-laying apparatus; hermaphrodite gonad; male sex myoblast; nervous system; and tail hypodermis. Used to study epilepsy. Human ortholog(s) of this gene implicated in autosomal dominant intellectual developmental disorder; autosomal dominant intellectual developmental disorder 44; cerebral atherosclerosis; cerebral infarction; and coronary artery disease. Orthologous to human KALRN (kalirin RhoGEF kinase) and TRIO (trio Rho guanine nucleotide exchange factor). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See unc-73 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 37

Sequence:: Chromosome: I; NC_003279.8 (3999300..4031739, complement)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

UNC-73, the C. elegans ortholog of Trio, is a guanine nucleotide exchange factor (GEF) upstream of both CED-10 and MIG-2 that is suppressed by CMRL1
Title: Polarized Rac-dependent protrusions drive epithelial intercalation in the embryonic epidermis of C. elegans.
we propose a bipartite model for UNC-73/TRIO activity in excretory canal extension.
Title: Distinct cell guidance pathways controlled by the Rac and Rho GEF domains of UNC-73/TRIO in Caenorhabditis elegans.
UNC-73 RhoGEF-2 isoforms are required for proper neurotransmitter signaling and may function in the DCV-mediated neuromodulatory regulation of locomotion rate.
Title: UNC-73/trio RhoGEF-2 activity modulates Caenorhabditis elegans motility through changes in neurotransmitter signaling upstream of the GSA-1/Galphas pathway.
The antagonistic effects of CRML-1 and UNC-73 can control the direction of growth cone migration by regulating the levels of the SAX-3 (a Robo homolog) guidance receptor.
Title: C. elegans CARMIL negatively regulates UNC-73/Trio function during neuronal development.
Trio's Rho-specific GEF domain (unc-73)is a major Galpha(q) effector that, together with PLCbeta, mediates the Galpha(q) signaling that drives the locomotion, egg laying, and growth.
Title: Trio's Rho-specific GEF domain is the missing Galpha q effector in C. elegans.
Mutants with impaired growth cone functions, such as unc-73C, often exhibit abnormal terminations and inappropriate trajectories at the distal ends of the M2 axons.
Title: A genetic analysis of axon guidance in the C elegans pharynx.
UNC-73 signals through RhoGEF-2 to regulate pharynx and vulva musculature and to modulate synaptic neurotransmission.
Title: The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the regulation of pharynx pumping and normal neurotransmission in C. elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F55C7.4 (e-PCR)
- UniSTS:311825

F55C7.7 (e-PCR)
- UniSTS:311826

F55C7.3 (e-PCR)
- UniSTS:314470

F55C7.2 (e-PCR)
- UniSTS:316799

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Rac protein signal transduction	IC Inferred by Curator more info	PubMed
involved_in axon guidance	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell migration	TAS Traceable Author Statement more info	PubMed
involved_in chemorepulsion of axon	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in egg-laying behavior	TAS Traceable Author Statement more info	PubMed
involved_in embryo development ending in birth or egg hatching	NAS Non-traceable Author Statement more info	PubMed
involved_in engulfment of apoptotic cell	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment or maintenance of actin cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male genitalia morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mesodermal cell migration	TAS Traceable Author Statement more info	PubMed
involved_in motor neuron axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nematode larval development	NAS Non-traceable Author Statement more info	PubMed
involved_in netrin-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron migration	IPI Inferred from Physical Interaction more info	PubMed
involved_in neuron migration	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of Rac protein signal transduction	IC Inferred by Curator more info	PubMed
involved_in positive regulation of Rho protein signal transduction	IC Inferred by Curator more info	PubMed
involved_in positive regulation of acetylcholine secretion, neurotransmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of actin filament polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of axon extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to synapse	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of dorsal/ventral axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic transmission, GABAergic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of vulval development	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in muscle cell projection	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: CRAL-TRIO domain-containing protein;DH domain-containing protein;Rho guanine nucleotide exchange factor 26;Triple functional domain protein

NP_001021496.1

Confirmed by transcript evidence

NP_001021497.1

Confirmed by transcript evidence

NP_001021498.1

Confirmed by transcript evidence

NP_001021499.1

Confirmed by transcript evidence

NP_001021500.1

Confirmed by transcript evidence

NP_001249512.1

Confirmed by transcript evidence

NP_001249513.1

Confirmed by transcript evidence

NP_001361940.1

Confirmed by transcript evidence

NP_001367474.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

3999300..4031739 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001026325.2 → NP_001021496.1 CRAL-TRIO domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001021496.1

Status: REVIEWED

UniProtKB/TrEMBL

O61528, O61529, P91339

Conserved Domains (11) summary

smart00060
Location:2395 → 2473

FN3; Fibronectin type 3 domain

smart00516
Location:19 → 156

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:1208 → 1383

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam07679
Location:2305 → 2391

I-set; Immunoglobulin I-set domain

cd13241
Location:1990 → 2128

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

pfam00169
Location:1419 → 1511

PH; PH domain

pfam00621
Location:1813 → 1986

RhoGEF; RhoGEF domain

cl02488
Location:422 → 568

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cl11960
Location:2335 → 2392

Ig; Immunoglobulin domain

cl17036
Location:1575 → 1634

SH3; Src Homology 3 domain superfamily

cl17171
Location:1391 → 1509

PH-like; Pleckstrin homology-like domain
NM_001375256.3 → NP_001361940.1 Rho guanine nucleotide exchange factor 26 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

H2KYM3

Conserved Domains (6) summary

smart00060
Location:874 → 952

FN3; Fibronectin type 3 domain

smart00326
Location:52 → 112

SH3; Src homology 3 domains

cd00160
Location:289 → 464

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13241
Location:469 → 607

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

pfam12300
Location:547 → 723

RhlB; ATP-dependent RNA helicase RhlB

cl11960
Location:784 → 870

Ig; Immunoglobulin domain
NM_001026327.4 → NP_001021498.1 Rho guanine nucleotide exchange factor 26 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001021498.1

Status: REVIEWED

UniProtKB/TrEMBL

Q7JNG6

Conserved Domains (6) summary

smart00060
Location:835 → 913

FN3; Fibronectin type 3 domain

smart00326
Location:13 → 73

SH3; Src homology 3 domains

cd00160
Location:250 → 425

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam07679
Location:745 → 831

I-set; Immunoglobulin I-set domain

cd13241
Location:430 → 568

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

cl11960
Location:775 → 832

Ig; Immunoglobulin domain
NM_001262583.3 → NP_001249512.1 Triple functional domain protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001249512.1

Status: REVIEWED

UniProtKB/TrEMBL

H2KYM4

Conserved Domains (5) summary

smart00060
Location:830 → 908

FN3; Fibronectin type 3 domain

cd00160
Location:245 → 420

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam07679
Location:740 → 826

I-set; Immunoglobulin I-set domain

cd13241
Location:425 → 563

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

cl11960
Location:770 → 827

Ig; Immunoglobulin domain
NM_001381834.2 → NP_001367474.1 CRAL-TRIO domain-containing protein [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

C4ALD5

Conserved Domains (6) summary

smart00516
Location:19 → 156

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:1810 → 1985

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13241
Location:1990 → 2128

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2

cl02488
Location:422 → 568

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cl17036
Location:1575 → 1634

SH3; Src Homology 3 domain superfamily

cl17171
Location:1391 → 1509

PH-like; Pleckstrin homology-like domain
NM_001262584.2 → NP_001249513.1 DH domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001249513.1

Status: REVIEWED

UniProtKB/TrEMBL

H2KYM2

Conserved Domains (3) summary

smart00326
Location:13 → 73

SH3; Src homology 3 domains

cd00160
Location:250 → 425

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13241
Location:430 → 568

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2
NM_001026329.7 → NP_001021500.1 DH domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001021500.1

Status: REVIEWED

UniProtKB/TrEMBL

Q95Q51

Conserved Domains (2) summary

cd00160
Location:245 → 420

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13241
Location:425 → 563

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2
NM_001026328.3 → NP_001021499.1 DH domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001021499.1

Status: REVIEWED

UniProtKB/TrEMBL

Q7JNG4

Conserved Domains (2) summary

cd00160
Location:164 → 339

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13241
Location:344 → 482

PH2_Kalirin_Trio_p63RhoGEF; p63RhoGEF pleckstrin homology (PH) domain, repeat 2
NM_001026326.4 → NP_001021497.1 CRAL-TRIO domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001021497.1

Status: REVIEWED

UniProtKB/TrEMBL

Q7KPP4

Conserved Domains (6) summary

smart00516
Location:19 → 156

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:1208 → 1383

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam00169
Location:1419 → 1511

PH; PH domain

cl02488
Location:422 → 568

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cl17036
Location:1575 → 1634

SH3; Src Homology 3 domain superfamily

cl17171
Location:1391 → 1509

PH-like; Pleckstrin homology-like domain