puf-8 PUM-HD domain-containing protein [Caenorhabditis elegans] - Gene

Summary

Go to the top of the page Help

Official Symbol: puf-8
Official Full Name: PUM-HD domain-containing protein
Primary source: WormBase:WBGene00004244
Locus tag: CELE_C30G12.7
See related: AllianceGenome:WB:WBGene00004244
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables mRNA 3'-UTR binding activity. Involved in male meiotic nuclear division; negative regulation of MAPK cascade; and spermatogenesis. Located in P granule and nuclear periphery. Is expressed in several structures, including P3.p hermaphrodite; P4.p hermaphrodite; germ line; sperm; and vulva. Used to study obesity. Human ortholog(s) of this gene implicated in cerebellar ataxia type 47. Orthologous to human PUM1 (pumilio RNA binding family member 1) and PUM2 (pumilio RNA binding family member 2). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See puf-8 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 6

Sequence:: Chromosome: II; NC_003280.10 (7296110..7298247, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PUF-8, a C. elegans ortholog of the RNA-binding proteins PUM1 and PUM2, is required for robustness of the cell death fate.
Title: PUF-8, a C. elegans ortholog of the RNA-binding proteins PUM1 and PUM2, is required for robustness of the cell death fate.
Genetic and Chemical Controls of Sperm Fate and Spermatocyte Dedifferentiation via PUF-8 and MPK-1 in Caenorhabditis elegans.
Title: Genetic and Chemical Controls of Sperm Fate and Spermatocyte Dedifferentiation via PUF-8 and MPK-1 in Caenorhabditis elegans.
Structural recognition of the mRNA 3' UTR by PUF-8 restricts the lifespan of C. elegans.
Title: Structural recognition of the mRNA 3' UTR by PUF-8 restricts the lifespan of C. elegans.
A PUF-8/PUM2-mediated layer of post-transcriptional regulation links altered Mff translation to mitochondrial dynamics and mitophagy, thereby mediating age-related mitochondrial dysfunctions.
Title: The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging.
Results reveal a role for the proteasome in chromosome tethering and identify PUF-8 as a regulator of proteasome activity during early meiosis.
Title: PUF-8 facilitates homologous chromosome pairing by promoting proteasome activity during meiotic entry in C. elegans.
Gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes.
Title: PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes in Caenorhabditis elegans.
PUF-8 negatively regulates RAS/MAPK signalling to promote differentiation of C. elegans germ cells.
Title: PUF-8 negatively regulates RAS/MAPK signalling to promote differentiation of C. elegans germ cells.
PUF-8 and TCER-1 are essential for normal levels of multiple mRNAs in the C. elegans germline.PUF proteins, in addition to their known cytoplasmic roles, participate in nuclear processing and/or export of mRNAs.
Title: PUF-8 and TCER-1 are essential for normal levels of multiple mRNAs in the C. elegans germline.
puf-8 likely inhibits the proliferative fate through negatively regulating GLP-1/Notch signaling or by functioning parallel to it.
Title: PUF-8, a Pumilio homolog, inhibits the proliferative fate in the Caenorhabditis elegans germline.
PUF-8 binds in vitro to specific sequences within pal-1 3' UTR that are critical for post-transcriptional suppression in germline stem cells.
Title: PUF-8 suppresses the somatic transcription factor PAL-1 expression in C. elegans germline stem cells.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

STS2-155 (e-PCR)
- UniSTS:241824

C30G12.7 (e-PCR)
- UniSTS:308673

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in post-transcriptional regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of translation	TAS Traceable Author Statement more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear periphery	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

Go to the top of the page Help

Preferred Names: PUM-HD domain-containing protein

NP_495523.3

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

7296110..7298247 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_063122.9 → NP_495523.3 PUM-HD domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495523.3

Status: REVIEWED

UniProtKB/TrEMBL

Q09487

Conserved Domains (1) summary

cd07920
Location:189 → 514

Pumilio; Pumilio-family RNA binding domain