amph-1 Amphiphysin [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: amph-1
Official Full Name: Amphiphysin
Primary source: WormBase:WBGene00010272
Locus tag: CELE_F58G6.1
See related: AllianceGenome:WB:WBGene00010272
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable phospholipid binding activity. Involved in lipid tube assembly involved in organelle fission; nucleus localization; and nucleus organization. Located in nuclear envelope; perinuclear region of cytoplasm; and recycling endosome. Is expressed in several structures, including coelomocyte; hypodermis; intestine; nervous system; and reproductive system. Human ortholog(s) of this gene implicated in breast carcinoma; centronuclear myopathy 2; and prostate adenocarcinoma. Orthologous to several human genes including BIN1 (bridging integrator 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See amph-1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 13

Sequence:: Chromosome: IV; NC_003282.8 (9644294..9647873, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GTP-stimulated membrane fission by the N-BAR protein AMPH-1.
Title: GTP-stimulated membrane fission by the N-BAR protein AMPH-1.
the loss of TBC-2 or AMPH-1 leads to abnormally high spatial overlap of RAB-5 and RAB-10. RAB-10 and AMPH-1 mediated down-regulation of RAB-5 is an important step in recycling, required for cargo exit from early endosomes
Title: Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes.
show that endogenous C. elegans AMPH-1 colocalizes with RME-1 on recycling endosomes in vivo, that amph-1-deletion mutants are defective in recycling endosome morphology and function.
Title: AMPH-1/Amphiphysin/Bin1 functions with RME-1/Ehd1 in endocytic recycling.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F58G6.1 (e-PCR)
- UniSTS:314534

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in lipid tube assembly involved in organelle fission	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleus localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleus organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in organelle	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Amphiphysin

NP_501711.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

9644294..9647873 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_069310.6 → NP_501711.1 Amphiphysin [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_501711.1

Status: REVIEWED

UniProtKB/TrEMBL

Q21004

Conserved Domains (3) summary

pfam16601
Location:252 → 390

NPF; Rabosyn-5 repeating NPF sequence-motif

cl12013
Location:22 → 240

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

cl17036
Location:400 → 461

SH3; Src Homology 3 domain superfamily