lem-3 Ankyrin repeat and LEM domain-containing protein 1 homolog [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: lem-3
Official Full Name: Ankyrin repeat and LEM domain-containing protein 1 homolog
Primary source: WormBase:WBGene00002276
Locus tag: CELE_F42H11.2
See related: AllianceGenome:WB:WBGene00002276
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables DNA endonuclease activity. Involved in several processes, including DNA metabolic process; cellular response to hydroxyurea; and response to UV-C. Located in several cellular components, including condensed chromosome; midbody; and mitotic spindle midzone. Is expressed in embryonic cell. Orthologous to human ANKLE1 (ankyrin repeat and LEM domain containing 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See lem-3 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 7

Sequence:: Chromosome: I; NC_003279.8 (9578526..9581267, complement)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that LEM-3 is able to process erroneous recombination intermediates that persist into the second meiotic division.
Title: The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans.
LEM-3 acts at the midbody, the structure where abscission occurs at the end of cytokinesis.
Title: LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis.
LEM-3 is a LEM domain containing nuclease involved in the DNA damage response in C. elegans
Title: LEM-3 - A LEM domain containing nuclease involved in the DNA damage response in C. elegans.

Submit: New GeneRIF Correction

General gene information

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Markers

F42H11.2 (e-PCR)
- UniSTS:321466

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables endonuclease activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA damage response	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA damage response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cellular response to hydroxyurea	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to ionizing radiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reproductive process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in resolution of meiotic recombination intermediates	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to UV	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to UV-C	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to X-ray	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to X-ray	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle midzone	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle midzone	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Ankyrin repeat and LEM domain-containing protein 1 homolog

NP_492539.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

9578526..9581267 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_060138.4 → NP_492539.2 Ankyrin repeat and LEM domain-containing protein 1 homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_492539.2

Status: REVIEWED

UniProtKB/Swiss-Prot

G5EGA3

Conserved Domains (5) summary

smart00540
Location:426 → 470

LEM; in nuclear membrane-associated proteins

cd10454
Location:527 → 634

GIY-YIG_COG3680_Meta; GIY-YIG domain of hypothetical proteins from Metazoa

cd00204
Location:32 → 122

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam13637
Location:31 → 84

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:32 → 61

ANK; ANK repeat [structural motif]