PolQ DNA polymerase theta [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: PolQprovided by FlyBase
Official Full Name: DNA polymerase thetaprovided by FlyBase
Primary source: FLYBASE:FBgn0002905
Locus tag: Dmel_CG6019
See related: AllianceGenome:FB:FBgn0002905
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0443/09; AAF54858; CG6019; Dmel\CG6019; DNApol-theta; mus-308; mus308; Mus308; Nuc3; Pol theta; polq; POLQ
Summary: Enables DNA-directed DNA polymerase activity. Involved in DNA metabolic process. Predicted to be active in mitochondrial nucleoid and nucleus. Is expressed in several structures, including anterior endoderm; anterior endoderm anlage; central brain primordium; egg chamber; and embryonic/larval midgut primordium. Orthologous to human POLQ (DNA polymerase theta). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 87C6-87C6; 3-52 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (12705523..12712299, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (8531245..8538021, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Persistent DNA damage signaling and DNA polymerase theta promote broken chromosome segregation.
Title: Persistent DNA damage signaling and DNA polymerase theta promote broken chromosome segregation.
Both polymerase and ATPase activities are required during microhomology-mediated end joining and interstrand crosslink repair.
Title: Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair.
DNA polymerase theta, encoded by the mus308 gene, plays a crucial role in DNA ligase 4-independent alternative end joining.
Title: Dual roles for DNA polymerase theta in alternative end-joining repair of double-strand breaks in Drosophila.
mus 308 gene product localizes throughout nuclei in wild-type cells but appears to be absent in a mus 308 mutant Drosophila
Title: The Drosophila mus 308 gene product, implicated in tolerance of DNA interstrand crosslinks, is a nuclear protein found in both ovaries and embryos.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm1973 (e-PCR)
- UniSTS:49864

Dm1718 (e-PCR)
- UniSTS:52954

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 5'-deoxyribose-5-phosphate lyase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA helicase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in DNA biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA synthesis involved in DNA repair	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA synthesis involved in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA-templated DNA replication	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via alternative nonhomologous end joining	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via alternative nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-excision repair involved in interstrand cross-link repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork processing	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in mitochondrial nucleoid	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA polymerase theta

Names: CG6019-PA; Nuclease-3; PolQ-PA; mutagen-sensitive 308; pol theta

NP_524333.1

EC 2.7.7.7

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

12705523..12712299 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079609.3 → NP_524333.1 DNA polymerase theta [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524333.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O18475, Q5BI65

Related

FBpp0082131

Conserved Domains (5) summary

cd08638
Location:1668 → 2053

DNA_pol_A_theta; DNA polymerase theta is a low-fidelity family A enzyme implicated in translesion synthesis and in somatic hypermutation

smart00490
Location:542 → 616

HELICc; helicase superfamily c-terminal domain

COG0749
Location:1489 → 2055

PolA; DNA polymerase I - 3'-5' exonuclease and polymerase domains [Replication, recombination and repair]

COG1204
Location:209 → 978

BRR2; Replicative superfamily II helicase [Replication, recombination and repair]

pfam00270
Location:251 → 404

DEAD; DEAD/DEAH box helicase