Mib2 MIB E3 ubiquitin protein ligase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mib2provided by RGD
Official Full Name: MIB E3 ubiquitin protein ligase 2provided by RGD
Primary source: RGD:1359469
See related: EnsemblRapid:ENSRNOG00000017564 AllianceGenome:RGD:1359469
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable ubiquitin protein ligase activity. Involved in regulation of postsynaptic neurotransmitter receptor internalization and regulation protein catabolic process at postsynapse. Is active in glutamatergic synapse and postsynapse. Orthologous to human MIB2 (MIB E3 ubiquitin protein ligase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 102.2), Adrenal (RPKM 66.0) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 5q36

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	5	NC_086023.1 (171526037..171542479, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	5	NC_051340.1 (166243776..166259944, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	5	NC_005104.4 (173082943..173099353, complement)

Chromosome 5 - NC_086023.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

sustained activation of glutamate receptors, a condition occurring in brain ischemia that down-regulates GABAB receptors, considerably increased the expression of MIB2 and Lys-63-linked ubiquitination of GABAB receptors. Interfering with Lys-63-linked ubiquitination by overexpressing ubiquitin mutants or GABAB1 mutants deficient in Lys-63-linked ubiquitination prevented glutamate-induced down-regulation of the receptors.
Title: Lys-63-linked Ubiquitination of γ-Aminobutyric Acid (GABA), Type B1, at Multiple Sites by the E3 Ligase Mind Bomb-2 Targets GABAB Receptors to Lysosomal Degradation.

Submit: New GeneRIF Correction

General gene information

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Markers

RH141852 (e-PCR)
- UniSTS:223844

Mib2 (e-PCR), detects polymorphism
- UniSTS:508529

Mib2 (e-PCR), detects polymorphism
- UniSTS:508366

Mib2 (e-PCR), detects polymorphism
- UniSTS:508368

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Notch signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic neurotransmitter receptor internalization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation protein catabolic process at postsynapse	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in early endosome	IEA Inferred from Electronic Annotation more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
part_of ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase MIB2

Names: RBSC-skeletrophin; RBSC-skeletrophin/dystrophin-like polypeptide; RING-type E3 ubiquitin transferase MIB2; mind bomb homolog 2; mindbomb E3 ubiquitin protein ligase 2

NP_001005901.2

EC 2.3.2.27

XP_006239656.1

EC 2.3.2.27

XP_017449042.1

EC 2.3.2.27

XP_017449043.1

EC 2.3.2.27

XP_017449044.1

EC 2.3.2.27

XP_017449047.1

EC 2.3.2.27

XP_063144230.1

EC 2.3.2.27

XP_063144231.1

EC 2.3.2.27

XP_063144232.1

EC 2.3.2.27

XP_063144233.1

EC 2.3.2.27

XP_063144235.1

EC 2.3.2.27

XP_063144236.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001005901.2 → NP_001005901.2 E3 ubiquitin-protein ligase MIB2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/TrEMBL

A0A140TAC8

Conserved Domains (8) summary

cd00204
Location:459 → 581

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd02339
Location:89 → 133

ZZ_Mind_bomb; Zinc finger, ZZ type. Zinc finger present in Drosophila Mind bomb (D-mib) and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Mind bomb is an E3 ubiqitin ligase that has ...

pfam06701
Location:160 → 224

MIB_HERC2; Mib_herc2

pfam10921
Location:255 → 333

DUF2710; Protein of unknown function (DUF2710)

pfam12796
Location:465 → 557

Ank_2; Ankyrin repeats (3 copies)

pfam13857
Location:548 → 603

Ank_5; Ankyrin repeats (many copies)

pfam13920
Location:905 → 948

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)

sd00045
Location:461 → 491

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086023.1 Reference GRCr8

Range

171526037..171542479 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017593554.3 → XP_017449043.1 E3 ubiquitin-protein ligase MIB2 isoform X6

UniProtKB/TrEMBL

A0A140TAC8, A0A8I5ZTL8

Conserved Domains (9) summary

PLN03192
Location:453 → 517

PLN03192; Voltage-dependent potassium channel; Provisional

PHA03095
Location:486 → 737

PHA03095; ankyrin-like protein; Provisional

cd02339
Location:89 → 133

ZZ_Mind_bomb; Zinc finger, ZZ type. Zinc finger present in Drosophila Mind bomb (D-mib) and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Mind bomb is an E3 ubiqitin ligase that has ...

sd00045
Location:490 → 520

ANK; ANK repeat [structural motif]

cd00162
Location:938 → 970

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cd16726
Location:858 → 894

RING1-HC_MIB2; RING finger 1, HC subclass, found in mind bomb 2 (MIB2) and similar proteins

pfam06701
Location:160 → 224

MIB_HERC2; Mib_herc2

pfam18346
Location:328 → 392

SH3_15; Mind bomb SH3 repeat domain

cl17238
Location:937 → 982

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
XM_017593553.3 → XP_017449042.1 E3 ubiquitin-protein ligase MIB2 isoform X6

UniProtKB/TrEMBL

A0A140TAC8, A0A8I5ZTL8

Conserved Domains (9) summary

PLN03192
Location:453 → 517

PLN03192; Voltage-dependent potassium channel; Provisional

PHA03095
Location:486 → 737

PHA03095; ankyrin-like protein; Provisional

cd02339
Location:89 → 133

ZZ_Mind_bomb; Zinc finger, ZZ type. Zinc finger present in Drosophila Mind bomb (D-mib) and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Mind bomb is an E3 ubiqitin ligase that has ...

sd00045
Location:490 → 520

ANK; ANK repeat [structural motif]

cd00162
Location:938 → 970

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cd16726
Location:858 → 894

RING1-HC_MIB2; RING finger 1, HC subclass, found in mind bomb 2 (MIB2) and similar proteins

pfam06701
Location:160 → 224

MIB_HERC2; Mib_herc2

pfam18346
Location:328 → 392

SH3_15; Mind bomb SH3 repeat domain

cl17238
Location:937 → 982

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
XM_063288161.1 → XP_063144231.1 E3 ubiquitin-protein ligase MIB2 isoform X3

UniProtKB/TrEMBL

A0A140TAC8
XM_017593555.3 → XP_017449044.1 E3 ubiquitin-protein ligase MIB2 isoform X6

UniProtKB/TrEMBL

A0A140TAC8, A0A8I5ZTL8

Related

ENSRNOP00000082241.1, ENSRNOT00000100849.2

Conserved Domains (9) summary

PLN03192
Location:453 → 517

PLN03192; Voltage-dependent potassium channel; Provisional

PHA03095
Location:486 → 737

PHA03095; ankyrin-like protein; Provisional

cd02339
Location:89 → 133

ZZ_Mind_bomb; Zinc finger, ZZ type. Zinc finger present in Drosophila Mind bomb (D-mib) and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Mind bomb is an E3 ubiqitin ligase that has ...

sd00045
Location:490 → 520

ANK; ANK repeat [structural motif]

cd00162
Location:938 → 970

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cd16726
Location:858 → 894

RING1-HC_MIB2; RING finger 1, HC subclass, found in mind bomb 2 (MIB2) and similar proteins

pfam06701
Location:160 → 224

MIB_HERC2; Mib_herc2

pfam18346
Location:328 → 392

SH3_15; Mind bomb SH3 repeat domain

cl17238
Location:937 → 982

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
XM_063288166.1 → XP_063144236.1 E3 ubiquitin-protein ligase MIB2 isoform X6

UniProtKB/TrEMBL

A0A140TAC8, A0A8I5ZTL8
XM_006239594.4 → XP_006239656.1 E3 ubiquitin-protein ligase MIB2 isoform X7

UniProtKB/TrEMBL

A0A140TAC8

Conserved Domains (7) summary

cd00204
Location:387 → 509

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam06701
Location:59 → 123

MIB_HERC2; Mib_herc2

pfam12796
Location:393 → 485

Ank_2; Ankyrin repeats (3 copies)

pfam13857
Location:476 → 531

Ank_5; Ankyrin repeats (many copies)

pfam13920
Location:833 → 876

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)

sd00045
Location:389 → 419

ANK; ANK repeat [structural motif]

cl00295
Location:1 → 32

ZZ; Zinc finger, ZZ type. Zinc finger present in dystrophin, CBP/p300 and many other proteins. The ZZ motif coordinates one or two zinc ions and most likely participates in ligand binding or molecular scaffolding. Many proteins containing ZZ motifs have ...
XM_063288165.1 → XP_063144235.1 E3 ubiquitin-protein ligase MIB2 isoform X6

UniProtKB/TrEMBL

A0A140TAC8, A0A8I5ZTL8
XM_063288163.1 → XP_063144233.1 E3 ubiquitin-protein ligase MIB2 isoform X5

UniProtKB/TrEMBL

A0A140TAC8, A6IUR4
XM_063288162.1 → XP_063144232.1 E3 ubiquitin-protein ligase MIB2 isoform X4

UniProtKB/TrEMBL

A0A140TAC8

Related

ENSRNOP00000023696.9, ENSRNOT00000023696.9
XM_063288160.1 → XP_063144230.1 E3 ubiquitin-protein ligase MIB2 isoform X1

UniProtKB/TrEMBL

A0A140TAC8
XM_017593558.3 → XP_017449047.1 E3 ubiquitin-protein ligase MIB2 isoform X2

UniProtKB/TrEMBL

A0A140TAC8

Conserved Domains (9) summary

PLN03192
Location:428 → 492

PLN03192; Voltage-dependent potassium channel; Provisional

PHA03095
Location:461 → 712

PHA03095; ankyrin-like protein; Provisional

cd02339
Location:89 → 133

ZZ_Mind_bomb; Zinc finger, ZZ type. Zinc finger present in Drosophila Mind bomb (D-mib) and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Mind bomb is an E3 ubiqitin ligase that has ...

sd00045
Location:465 → 495

ANK; ANK repeat [structural motif]

cd00162
Location:913 → 945

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cd16726
Location:833 → 869

RING1-HC_MIB2; RING finger 1, HC subclass, found in mind bomb 2 (MIB2) and similar proteins

pfam06701
Location:160 → 224

MIB_HERC2; Mib_herc2

pfam18346
Location:328 → 392

SH3_15; Mind bomb SH3 repeat domain

cl17238
Location:912 → 957

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain