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    CDC27 cell division cycle 27 [ Homo sapiens (human) ]

    Gene ID: 996, updated on 10-Dec-2024

    Summary

    Official Symbol
    CDC27provided by HGNC
    Official Full Name
    cell division cycle 27provided by HGNC
    Primary source
    HGNC:HGNC:1728
    See related
    Ensembl:ENSG00000004897 MIM:116946; AllianceGenome:HGNC:1728
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
    Summary
    The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]
    Expression
    Ubiquitous expression in thyroid (RPKM 20.3), testis (RPKM 11.6) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See CDC27 in Genome Data Viewer
    Location:
    17q21.32
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 17 NC_000017.11 (47117703..47189295, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 17 NC_060941.1 (47979197..48051640, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (45195069..45266661, complement)

    Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene leucine rich repeat containing 37 member A2 Neighboring gene uncharacterized LOC101927060 Neighboring gene leucine rich repeat containing 37 member A17, pseudogene Neighboring gene MPRA-validated peak2870 silencer Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:45145515-45146016 Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:45146017-45146516 Neighboring gene uncharacterized LOC124904015 Neighboring gene RNA, 7SL, cytoplasmic 270, pseudogene Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:45175453-45176078 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8621 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr17:45197166-45197776 Neighboring gene protein GVQW1-like Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr17:45213296-45214495 Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:45266269-45266865 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8622 Neighboring gene CRISPRi-validated cis-regulatory element chr17.3215 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12300 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12301 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12302 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12303 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12304 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8623 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr17:45298098-45298754 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:45298755-45299410 Neighboring gene ribosomal protein S2 pseudogene 47 Neighboring gene Sharpr-MPRA regulatory region 4457 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12305 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:45314666-45315602 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:45315603-45316539 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8624 Neighboring gene myosin light chain 4 Neighboring gene RNA, U7 small nuclear 186 pseudogene Neighboring gene integrin subunit beta 3

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein phosphatase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Component Evidence Code Pubs
    part_of anaphase-promoting complex IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    part_of anaphase-promoting complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of anaphase-promoting complex IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    part_of anaphase-promoting complex NAS
    Non-traceable Author Statement
    more info
    PubMed 
    located_in centrosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in mitotic spindle IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in spindle IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    cell division cycle protein 27 homolog
    Names
    D0S1430E, D17S978E
    anaphase promoting complex subunit 3
    anaphase-promoting complex, protein 3
    cell division cycle 27 homolog
    nuc2 homolog

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001114091.4NP_001107563.1  cell division cycle protein 27 homolog isoform 1

      See identical proteins and their annotated locations for NP_001107563.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AA927469, AC002558, BC011656, HY023336
      Consensus CDS
      CCDS45720.1
      UniProtKB/Swiss-Prot
      P30260
      Related
      ENSP00000434614.1, ENST00000531206.5
      Conserved Domains (5) summary
      COG0457
      Location:507768
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:573601
      TPR; TPR repeat [structural motif]
      pfam00515
      Location:641673
      TPR_1; Tetratricopeptide repeat
      pfam12895
      Location:1792
      ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3
      pfam13424
      Location:463534
      TPR_12; Tetratricopeptide repeat
    2. NM_001256.6NP_001247.3  cell division cycle protein 27 homolog isoform 2

      See identical proteins and their annotated locations for NP_001247.3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.
      Source sequence(s)
      AA927469, AC002558, HY023336, S78234
      Consensus CDS
      CCDS11509.1
      UniProtKB/Swiss-Prot
      G3V1C4, P30260, Q16349, Q96F35
      Related
      ENSP00000066544.3, ENST00000066544.8
      Conserved Domains (3) summary
      COG0457
      Location:501762
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:567595
      TPR; TPR repeat [structural motif]
      pfam12895
      Location:1792
      ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3
    3. NM_001293089.3NP_001280018.1  cell division cycle protein 27 homolog isoform 3

      See identical proteins and their annotated locations for NP_001280018.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1.
      Source sequence(s)
      AA927469, AC002558, HY023336, U00001
      Consensus CDS
      CCDS74090.1
      UniProtKB/TrEMBL
      G5EA36
      Related
      ENSP00000437339.1, ENST00000527547.5
      Conserved Domains (5) summary
      COG0457
      Location:500761
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:566594
      TPR; TPR repeat [structural motif]
      pfam00515
      Location:634666
      TPR_1; Tetratricopeptide repeat
      pfam12895
      Location:1792
      ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3
      pfam13424
      Location:457527
      TPR_12; Tetratricopeptide repeat
    4. NM_001293091.3NP_001280020.1  cell division cycle protein 27 homolog isoform 4

      See identical proteins and their annotated locations for NP_001280020.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) lacks an alternate exon in the 5' region and it thus differs in its 5' UTR and initiates translation at an alternate start codon, and it also uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1.
      Source sequence(s)
      AA927469, AC002558, AK296881, HY023336
      UniProtKB/TrEMBL
      B4DL80, E7EN31
      Conserved Domains (2) summary
      COG0457
      Location:440701
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:506534
      TPR; TPR repeat [structural motif]
    5. NM_001353035.2NP_001339964.1  cell division cycle protein 27 homolog isoform 5

      Status: REVIEWED

      Source sequence(s)
      AC002558
      Conserved Domains (3) summary
      COG0457
      Location:501759
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:567595
      TPR; TPR repeat [structural motif]
      pfam12895
      Location:1792
      ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3
    6. NM_001353047.2NP_001339976.1  cell division cycle protein 27 homolog isoform 6

      Status: REVIEWED

      Source sequence(s)
      AC002558
      Conserved Domains (3) summary
      COG0457
      Location:500758
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:566594
      TPR; TPR repeat [structural motif]
      pfam12895
      Location:1792
      ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3
    7. NM_001353049.2NP_001339978.1  cell division cycle protein 27 homolog isoform 7

      Status: REVIEWED

      Source sequence(s)
      AC002558
      Conserved Domains (2) summary
      COG0457
      Location:440698
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:506534
      TPR; TPR repeat [structural motif]
    8. NM_001353050.2NP_001339979.1  cell division cycle protein 27 homolog isoform 8

      Status: REVIEWED

      Source sequence(s)
      AC002558
      Conserved Domains (2) summary
      COG0457
      Location:404665
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:470498
      TPR; TPR repeat [structural motif]
    9. NM_001353051.2NP_001339980.1  cell division cycle protein 27 homolog isoform 9

      Status: REVIEWED

      Source sequence(s)
      AC002558
      Conserved Domains (3) summary
      COG0457
      Location:56303
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      TIGR02917
      Location:182414
      PEP_TPR_lipo; putative PEP-CTERM system TPR-repeat lipoprotein
      sd00006
      Location:169197
      TPR; TPR repeat [structural motif]

    RNA

    1. NR_148340.2 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AC002558

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000017.11 Reference GRCh38.p14 Primary Assembly

      Range
      47117703..47189295 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017025485.3XP_016880974.1  cell division cycle protein 27 homolog isoform X7

    2. XM_017025484.3XP_016880973.1  cell division cycle protein 27 homolog isoform X6

    3. XM_047437230.1XP_047293186.1  cell division cycle protein 27 homolog isoform X5

    4. XM_011525548.4XP_011523850.1  cell division cycle protein 27 homolog isoform X4

      See identical proteins and their annotated locations for XP_011523850.1

      Conserved Domains (5) summary
      COG0457
      Location:446707
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:512540
      TPR; TPR repeat [structural motif]
      pfam00515
      Location:580612
      TPR_1; Tetratricopeptide repeat
      pfam13181
      Location:684714
      TPR_8; Tetratricopeptide repeat
      pfam13424
      Location:402473
      TPR_12; Tetratricopeptide repeat
    5. XM_011525546.4XP_011523848.1  cell division cycle protein 27 homolog isoform X1

      Conserved Domains (5) summary
      COG0457
      Location:506767
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:572600
      TPR; TPR repeat [structural motif]
      pfam00515
      Location:640672
      TPR_1; Tetratricopeptide repeat
      pfam12895
      Location:1792
      ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3
      pfam13424
      Location:463533
      TPR_12; Tetratricopeptide repeat
    6. XM_011525549.3XP_011523851.1  cell division cycle protein 27 homolog isoform X4

      See identical proteins and their annotated locations for XP_011523851.1

      Conserved Domains (5) summary
      COG0457
      Location:446707
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:512540
      TPR; TPR repeat [structural motif]
      pfam00515
      Location:580612
      TPR_1; Tetratricopeptide repeat
      pfam13181
      Location:684714
      TPR_8; Tetratricopeptide repeat
      pfam13424
      Location:402473
      TPR_12; Tetratricopeptide repeat
    7. XM_011525547.4XP_011523849.1  cell division cycle protein 27 homolog isoform X3

      Conserved Domains (5) summary
      COG0457
      Location:507765
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00006
      Location:573601
      TPR; TPR repeat [structural motif]
      pfam00515
      Location:641673
      TPR_1; Tetratricopeptide repeat
      pfam12895
      Location:1792
      ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3
      pfam13424
      Location:463534
      TPR_12; Tetratricopeptide repeat
    8. XM_047437229.1XP_047293185.1  cell division cycle protein 27 homolog isoform X2

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060941.1 Alternate T2T-CHM13v2.0

      Range
      47979197..48051640 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054318107.1XP_054174082.1  cell division cycle protein 27 homolog isoform X7

    2. XM_054318106.1XP_054174081.1  cell division cycle protein 27 homolog isoform X6

    3. XM_054318105.1XP_054174080.1  cell division cycle protein 27 homolog isoform X5

    4. XM_054318103.1XP_054174078.1  cell division cycle protein 27 homolog isoform X4

    5. XM_054318100.1XP_054174075.1  cell division cycle protein 27 homolog isoform X1

    6. XM_054318104.1XP_054174079.1  cell division cycle protein 27 homolog isoform X4

    7. XM_054318102.1XP_054174077.1  cell division cycle protein 27 homolog isoform X3

    8. XM_054318101.1XP_054174076.1  cell division cycle protein 27 homolog isoform X2