Chek2 checkpoint kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chek2provided by MGI
Official Full Name: checkpoint kinase 2provided by MGI
Primary source: MGI:MGI:1355321
See related: Ensembl:ENSMUSG00000029521 AllianceGenome:MGI:1355321
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CHK2; Cds1; Rad53; HUCDS1
Summary: Enables protein kinase activity. Acts upstream of or within several processes, including cellular response to gamma radiation; signal transduction by p53 class mediator; and thymocyte apoptotic process. Predicted to be located in Golgi apparatus and PML body. Predicted to be active in cytoplasm and nucleus. Predicted to colocalize with chromosome, telomeric region. Is expressed in several structures, including central nervous system; genitourinary system; and liver. Human ortholog(s) of this gene implicated in several diseases, including Li-Fraumeni syndrome 2; breast cancer (multiple); colorectal cancer; osteosarcoma; and prostate cancer. Orthologous to human CHEK2 (checkpoint kinase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in liver E14 (RPKM 9.9), liver E14.5 (RPKM 8.7) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 53.73 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (110987668..111022006)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (110839800..110874140)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Checkpoint kinase 2 controls insulin secretion and glucose homeostasis.
Title: Checkpoint kinase 2 controls insulin secretion and glucose homeostasis.
Chk2 Modulates Bmi1-Deficiency-Induced Renal Aging and Fibrosis via Oxidative Stress, DNA Damage, and p53/TGFbeta1-Induced Epithelial-Mesenchymal Transition.
Title: Chk2 Modulates Bmi1-Deficiency-Induced Renal Aging and Fibrosis via Oxidative Stress, DNA Damage, and p53/TGFβ1-Induced Epithelial-Mesenchymal Transition.
CHEK2 deficiency increase the response to PD-1 inhibitors by affecting the tumor immune microenvironment.
Title: CHEK2 deficiency increase the response to PD-1 inhibitors by affecting the tumor immune microenvironment.
MiR-669b-5p inhibits the Alzheimer's disease development via regulation of CHEK2 in Neuro2a APPSwe/Delta9 cells.
Title: MiR-669b-5p inhibits the Alzheimer's disease development via regulation of CHEK2 in Neuro2a APPSwe/Δ9 cells.
Checkpoint kinase 1/2 inhibition potentiates anti-tumoral immune response and sensitizes gliomas to immune checkpoint blockade.
Title: Checkpoint kinase 1/2 inhibition potentiates anti-tumoral immune response and sensitizes gliomas to immune checkpoint blockade.
Cell cycle kinase CHEK2 in macrophages alleviates the inflammatory response to Staphylococcus aureus-induced pneumonia.
Title: Cell cycle kinase CHEK2 in macrophages alleviates the inflammatory response to Staphylococcus aureus-induced pneumonia.
Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
Title: Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
SIRT1 modulates cell cycle progression by regulating CHK2 acetylation-phosphorylation.
Title: SIRT1 modulates cell cycle progression by regulating CHK2 acetylation-phosphorylation.
FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2.
Title: FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2.
CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos.
Title: CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos.

Submit: New GeneRIF Correction See all GeneRIFs (50)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Chek2 (e-PCR), detects polymorphism
- UniSTS:472836

NoName (e-PCR)
- UniSTS:235258

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA damage response	ISO Inferred from Sequence Orthology more info	PubMed
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in G2/M transition of mitotic cell cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cellular response to bisphenol A	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to gamma radiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair	ISS Inferred from Sequence or Structural Similarity more info
involved_in intrinsic apoptotic signaling pathway in response to DNA damage	ISO Inferred from Sequence Orthology more info
involved_in intrinsic apoptotic signaling pathway in response to DNA damage	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within mitotic G2 phase	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
involved_in mitotic intra-S DNA damage checkpoint signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitotic spindle assembly	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of DNA damage checkpoint	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA damage checkpoint	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of anoikis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of anoikis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of autophagosome assembly	IEA Inferred from Electronic Annotation more info
involved_in regulation of autophagosome assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within response to gamma radiation	IDA Inferred from Direct Assay more info	PubMed
involved_in response to glycoside	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within signal transduction in response to DNA damage	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within signal transduction in response to DNA damage	ISO Inferred from Sequence Orthology more info
involved_in signal transduction in response to DNA damage	ISO Inferred from Sequence Orthology more info
involved_in signal transduction in response to DNA damage	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within thymocyte apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in PML body	ISO Inferred from Sequence Orthology more info
located_in PML body	ISS Inferred from Sequence or Structural Similarity more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
colocalizes_with chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: serine/threonine-protein kinase Chk2

Names: CHK2 checkpoint homolog; Cds1 homolog; Rad53 homolog

NP_001350237.1

EC 2.7.11.1

NP_001412508.1

EC 2.7.11.1

NP_001412509.1

EC 2.7.11.1

NP_057890.1

EC 2.7.11.1

XP_006535131.3

EC 2.7.11.1

XP_006535133.1

EC 2.7.11.1

XP_006535134.3

EC 2.7.11.1

XP_030110520.1

EC 2.7.11.1

XP_036021156.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001363308.1 → NP_001350237.1 serine/threonine-protein kinase Chk2 isoform 2

Status: VALIDATED

Source sequence(s)

AC121934

Conserved Domains (1) summary

cd14084
Location:1 → 251

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
NM_001425579.1 → NP_001412508.1 serine/threonine-protein kinase Chk2 isoform 3

Status: VALIDATED

Source sequence(s)

AC121934
NM_001425580.1 → NP_001412509.1 serine/threonine-protein kinase Chk2 isoform 1

Status: VALIDATED

Source sequence(s)

AC121934

UniProtKB/Swiss-Prot

Q9Z265

UniProtKB/TrEMBL

Q543W6
NM_016681.4 → NP_057890.1 serine/threonine-protein kinase Chk2 isoform 1

See identical proteins and their annotated locations for NP_057890.1

Status: VALIDATED

Source sequence(s)

AK044913, BX512104, CJ079787

Consensus CDS

CCDS19533.1

UniProtKB/Swiss-Prot

Q9Z265

UniProtKB/TrEMBL

Q543W6

Related

ENSMUSP00000066679.2, ENSMUST00000066160.3

Conserved Domains (2) summary

cd00060
Location:97 → 205

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:217 → 490

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

RNA

NR_189322.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC121934

Related

ENSMUST00000199937.5
NR_189323.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC121934

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

110987668..111022006

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006535071.5 → XP_006535134.3 serine/threonine-protein kinase Chk2 isoform X4

Conserved Domains (2) summary

cd00060
Location:157 → 265

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cl21453
Location:277 → 484

PKc_like; Protein Kinases, catalytic domain
XM_006535068.5 → XP_006535131.3 serine/threonine-protein kinase Chk2 isoform X2

Conserved Domains (2) summary

cd00060
Location:157 → 265

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:277 → 550

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
XM_006535070.2 → XP_006535133.1 serine/threonine-protein kinase Chk2 isoform X3

See identical proteins and their annotated locations for XP_006535133.1

UniProtKB/Swiss-Prot

Q9Z265

UniProtKB/TrEMBL

Q543W6

Conserved Domains (2) summary

cd00060
Location:97 → 205

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:217 → 490

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
XM_036165263.1 → XP_036021156.1 serine/threonine-protein kinase Chk2 isoform X5

Conserved Domains (2) summary

cd00060
Location:97 → 205

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cl21453
Location:217 → 424

PKc_like; Protein Kinases, catalytic domain
XM_030254660.1 → XP_030110520.1 serine/threonine-protein kinase Chk2 isoform X6

Conserved Domains (1) summary

cd14084
Location:1 → 251

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2