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    Dhfr Dihydrofolate reductase [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 42003, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Dhfrprovided by FlyBase
    Official Full Name
    Dihydrofolate reductaseprovided by FlyBase
    Primary source
    FLYBASE:FBgn0004087
    Locus tag
    Dmel_CG14887
    See related
    AllianceGenome:FB:FBgn0004087
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG14887; dhfr; DHFR; Dmel\CG14887
    Summary
    Enables dihydrofolate reductase activity. Predicted to be involved in dihydrofolate metabolic process; folic acid metabolic process; and tetrahydrofolate biosynthetic process. Predicted to be active in mitochondrion. Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia. Orthologous to human DHFR (dihydrofolate reductase); DHFR2 (dihydrofolate reductase 2); and DHFRP1 (dihydrofolate reductase pseudogene 1). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See Dhfr in Genome Data Viewer
    Location:
    89B18-89B18; 3-58 cM
    Exon count:
    2
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (16481167..16481878)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (12306889..12307600)

    Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene antisense RNA:CR46029 Neighboring gene Guanylyl cyclase at 89Da Neighboring gene Guanylyl cyclase at 89Db Neighboring gene Succinate dehydrogenase assembly factor 3 Neighboring gene Derlin-2 Neighboring gene Cadherin 89D

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Selection for methotrexate resistance in mammalian cells bearing a Drosophila dihydrofolate reductase transgene: Methotrexate resistance in transgenic mammalian cells. Affleck JG, et al. Cell Biol Toxicol, 2010 Apr. PMID 19337845
    2. A single point mutation in Drosophila dihydrofolate reductase confers methotrexate resistance to a transgenic CHO cell line. Neumann K, et al. Genome, 2003 Aug. PMID 12897877
    3. The purification of dihydrofolate reductase from Drosophila melanogaster. Rancourt SL, et al. Biochim Biophys Acta, 1990 Jul 6. PMID 2116172
    4. Isolation and characterization of a dihydrofolate reductase gene mutation in methotrexate-resistant Drosophila cells. Hao H, et al. Gene Expr, 1996. PMID 9196078, Free PMC Article
    5. Dihydrofolate reductase of Drosophila. Cloning and expression of a gene with a rare transcript. Hao H, et al. J Biol Chem, 1994 May 27. PMID 8195153

    See all (42) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables NADP binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables NADP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables dihydrofolate reductase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables dihydrofolate reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables dihydrofolate reductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in dihydrofolate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in folic acid metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tetrahydrofolate biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tetrahydrofolate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in mitochondrial matrix ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    dihydrofolate reductase
    Names
    CG14887-PA
    CG14887-PB
    Dhfr-PA
    Dhfr-PB
    NP_001036720.1
    NP_732147.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033777.3 Reference assembly

      Range
      16481167..16481878
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001043255.2 → NP_001036720.1  dihydrofolate reductase, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001036720.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A4V302, P17719, Q9VEU4
      UniProtKB/TrEMBL
      G4LU61
      Conserved Domains (1) summary
      cd00209
      Location:4 → 179
      DHFR; Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5, ...

    2. NM_169721.2 → NP_732147.1  dihydrofolate reductase, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_732147.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A4V302, P17719, Q9VEU4
      UniProtKB/TrEMBL
      G4LU61
      Related
      FBpp0082746
      Conserved Domains (1) summary
      cd00209
      Location:4 → 179
      DHFR; Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5, ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P17719.3 P17719

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