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    Rhau RHAU helicase [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 35339, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Rhauprovided by FlyBase
    Official Full Name
    RHAU helicaseprovided by FlyBase
    Primary source
    FLYBASE:FBgn0032883
    Locus tag
    Dmel_CG9323
    See related
    AllianceGenome:FB:FBgn0032883
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    38E.15; cg9323; CG9323; DmDHX36; Dmel\CG9323; DmRHAU; RHAU
    Summary
    Enables DNA helicase activity; G-quadruplex RNA binding activity; and RNA helicase activity. Involved in G-quadruplex DNA unwinding. Predicted to be active in cytoplasm and nucleus. Is expressed in organism and spermatozoon. Used to study candidiasis. Orthologous to human DHX36 (DEAH-box helicase 36). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See Rhau in Genome Data Viewer
    Location:
    38E7-38E7; 2-54 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2L NT_033779.5 (20754579..20757985, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2L NT_033779.4 (20754579..20757985, complement)

    Chromosome 2L - NT_033779.5Genomic Context describing neighboring genes Neighboring gene Transmembrane 9 superfamily protein member 2 Neighboring gene Female sterile (2) Ketel Neighboring gene Alpha-methylacyl-CoA racemase Neighboring gene Nucleostemin 4 Neighboring gene diaphanous Neighboring gene caudal

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. RNAi-mediated knockdown of the Rhau helicase preferentially depletes proteins with a Guanine-quadruplex motif in the 5'-UTR of their mRNA. Vester K, et al. Biochem Biophys Res Commun, 2019 Jan 15. PMID 30528389
    2. Molecular Mechanistic Insights into Drosophila DHX36-Mediated G-Quadruplex Unfolding: A Structure-Based Model. Chen WF, et al. Structure, 2018 Mar 6. PMID 29429875
    3. A comparison study of pathological features and drug efficacy between Drosophila models of C9orf72 ALS/FTD. Lee D, et al. Mol Cells, 2024 Jan. PMID 38376483, Free PMC Article
    4. RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis. You H, et al. Nucleic Acids Res, 2017 Jan 9. PMID 28069994, Free PMC Article
    5. Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. Lattmann S, et al. Nucleic Acids Res, 2010 Oct. PMID 20472641, Free PMC Article

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. This indicates that in an unexpectedly small set of genes, in which G-quadruplex motifs are unusually common in the 5'-UTR of their mRNAs, Rhau helicase is responsible for the regulation of their expression.
      Title: RNAi-mediated knockdown of the Rhau helicase preferentially depletes proteins with a Guanine-quadruplex motif in the 5'-UTR of their mRNA.
    2. The crystal structures of DHX36 in complex with RNA and DNA, revealing that G-rich tracts are specifically recognized by the helicase. The negatively charged G-quadruplex is recognized and destabilized by a positively charged binding cage and then unfolded via ATP-dependent ssDNA translocation activity. On the basis of these results, a conceptual G4 DNA-resolving mechanism is proposed.
      Title: Molecular Mechanistic Insights into Drosophila DHX36-Mediated G-Quadruplex Unfolding: A Structure-Based Model.
    3. The study provides the first evidence that the unfolding kinetics of a G-quadruplex can be modulated by different nucleotide-bound states of the RHAU helicase (CG9323).
      Title: RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.
    4. CG9323 is the true ortholog of the human RHAU/DHX36 DEAH-box RNA helicase. CG9323 possesses guanine quadruplex-RNA binding and resolving activities.
      Title: Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables G-quadruplex DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables G-quadruplex RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables G-quadruplex RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables helicase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in G-quadruplex DNA unwinding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    RHAU helicase
    Names
    CG9323-PA
    CG9323-PB
    Rhau-PA
    Rhau-PB
    NP_001260639.1
    NP_610056.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033779.5 Reference assembly

      Range
      20754579..20757985 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001273710.1 → NP_001260639.1  RHAU helicase, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001260639.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8SWT2, Q9VIJ8
      Conserved Domains (5) summary
      smart00487
      Location:147 → 328
      DEXDc; DEAD-like helicases superfamily
      smart00847
      Location:614 → 696
      HA2; Helicase associated domain (HA2) Add an annotation
      cd00046
      Location:170 → 312
      DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
      pfam00271
      Location:411 → 544
      Helicase_C; Helicase conserved C-terminal domain
      pfam07717
      Location:735 → 853
      OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold

    2. NM_136212.2 → NP_610056.1  RHAU helicase, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_610056.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8SWT2, Q9VIJ8
      Related
      FBpp0080988
      Conserved Domains (5) summary
      smart00487
      Location:147 → 328
      DEXDc; DEAD-like helicases superfamily
      smart00847
      Location:614 → 696
      HA2; Helicase associated domain (HA2) Add an annotation
      cd00046
      Location:170 → 312
      DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.
      pfam00271
      Location:411 → 544
      Helicase_C; Helicase conserved C-terminal domain
      pfam07717
      Location:735 → 853
      OB_NTP_bind; Oligonucleotide/oligosaccharide-binding (OB)-fold
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8SWT2 Q8SWT2

    Gene LinkOut

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