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    TIL1 DNA polymerase epsilon catalytic subunit [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 837346, updated on 18-Sep-2024

    Summary

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    Official Symbol
    TIL1
    Official Full Name
    DNA polymerase epsilon catalytic subunit
    Primary source
    TAIR:AT1G08260
    Locus tag
    AT1G08260
    See related
    Araport:AT1G08260
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ABA OVERLY SENSITIVE 4; ABO4; EARLY IN SHORT DAYS 7; EMB142; EMB2284; EMB529; EMBRYO DEFECTIVE 142; EMBRYO DEFECTIVE 2284; EMBRYO DEFECTIVE 529; ESD7; POL2A; T23G18.21; T23G18_21; TILTED 1
    Summary
    Similar to POL2A, DNA polymerase epsilon catalytic subunit. Essential for Arabidopsis growth. Null homozygotes are embryo lethal, partial loss of function alleles show embryo patterning defects such as root pole displacement. Delayed progression through cell cycle results in embryos with smaller numbers of larger cells.
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    Genomic context

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    See TIL1 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    49
    Sequence:
    Chromosome: 1; NC_003070.9 (2588854..2606892)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene Transmembrane amino acid transporter family protein Neighboring gene tRNA-Gly Neighboring gene arogenate dehydratase 6 Neighboring gene vacuolar protein sorting-associated protein Neighboring gene Glycosyltransferase family 29 (sialyltransferase) family protein Neighboring gene WIP domain protein 3

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis. Bourguet P, et al. Genome Biol, 2020 Nov 25. PMID 33234150, Free PMC Article
    2. Formation of interference-sensitive meiotic cross-overs requires sufficient DNA leading-strand elongation. Huang J, et al. Proc Natl Acad Sci U S A, 2015 Oct 6. PMID 26392549, Free PMC Article
    3. Epigenetic regulation, somatic homologous recombination, and abscisic acid signaling are influenced by DNA polymerase epsilon mutation in Arabidopsis. Yin H, et al. Plant Cell, 2009 Feb. PMID 19244142, Free PMC Article
    4. The plant DNA polymerase theta is essential for the repair of replication-associated DNA damage. Nisa M, et al. Plant J, 2021 Jun. PMID 33989439
    5. Function of the Plant DNA Polymerase Epsilon in Replicative Stress Sensing, a Genetic Analysis. Pedroza-García JA, et al. Plant Physiol, 2017 Mar. PMID 28153919, Free PMC Article

    See all (13) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. DNA polymerase epsilon binds histone H3.1-H4 and recruits MORC1 to mediate meiotic heterochromatin condensation.
      Title: DNA polymerase epsilon binds histone H3.1-H4 and recruits MORC1 to mediate meiotic heterochromatin condensation.
    2. DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot in Arabidopsis.
      Title: DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot in Arabidopsis.
    3. The plant DNA polymerase theta is essential for the repair of replication-associated DNA damage.
      Title: The plant DNA polymerase theta is essential for the repair of replication-associated DNA damage.
    4. DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis.
      Title: DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis.
    5. Results indicate that the role of Pol epsilon in replicative stress sensing is conserved in plants.
      Title: Function of the Plant DNA Polymerase Epsilon in Replicative Stress Sensing, a Genetic Analysis.
    6. We also demonstrate that a domain of the C-terminal region of ESD7 mediates the binding to the different PRC2 components and this interaction is necessary for the proper recruitment of PRC2 to FT and SOC1 chromatin.
      Title: Arabidopsis DNA polymerase ϵ recruits components of Polycomb repressor complex to mediate epigenetic gene silencing.
    7. Data indicate that mutations in DNA polymerase epsilon catalytic subunit A (POL2A) cause reduced fertility and meiotic defects, including abnormal chromosome association, improper chromosome segregation, and fragmentation.
      Title: Formation of interference-sensitive meiotic cross-overs requires sufficient DNA leading-strand elongation.
    8. The ESD7 gene encodes the catalytic subunit of DNA polymerase epsilon. The esd7-1 mutant is early flowering and displays pleiotropic defects in both vegetative and reproductive development. [ESD7]
      Title: EARLY IN SHORT DAYS 7 (ESD7) encodes the catalytic subunit of DNA polymerase epsilon and is required for flowering repression through a mechanism involving epigenetic gene silencing.
    9. POL2a is involved in maintaining epigenetic states, homologous recombination, and ABA signaling in Arabidopsis.
      Title: Epigenetic regulation, somatic homologous recombination, and abscisic acid signaling are influenced by DNA polymerase epsilon mutation in Arabidopsis.
    10. role of DNA polymerase epsilon during gametophyte and seed development in A. thaliana, encoded by POL2a and POl2b, with a main regulatory subunit DPB2, is reported
      Title: Genetic analysis of two Arabidopsis DNA polymerase epsilon subunits during early embryogenesis.
    Submit: New GeneRIF Correction

    General gene information

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    NM_001331765.1
    NM_100699.6

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within embryo development ending in seed dormancy IMP
    Inferred from Mutant Phenotype
    more info
    		  
    acts_upstream_of_or_within embryo development ending in seed dormancy NAS
    Non-traceable Author Statement
    more info
    		  
    acts_upstream_of_or_within embryonic root morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of long-day photoperiodism, flowering IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of cell division IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in apoplast HDA PubMed 
    part_of epsilon DNA polymerase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of epsilon DNA polymerase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    DNA polymerase epsilon catalytic subunit
    NP_172303.5
    • TILTED 1 (TIL1); FUNCTIONS IN: DNA-directed DNA polymerase activity, DNA binding, nucleotide binding, zinc ion binding, nucleic acid binding; INVOLVED IN: positive regulation of S phase of mitotic cell cycle, negative regulation of long-day photoperiodism, flowering, embryo development ending in seed dormancy; LOCATED IN: apoplast, epsilon DNA polymerase complex; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: DNA polymerase, family B (InterPro:IPR022762), DNA-directed DNA polymerase, family B, exonuclease domain (InterPro:IPR006133), Domain of unknown function DUF1744 (InterPro:IPR013697), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337), DNA-directed DNA polymerase, family B, conserved region (InterPro:IPR006134); BEST Arabidopsis thaliana protein match is: DNA polymerase epsilon catalytic subunit (TAIR:AT2G27120.1); Has 2011 Blast hits to 1700 proteins in 652 species: Archae - 331; Bacteria - 603; Metazoa - 305; Fungi - 291; Plants - 83; Viruses - 58; Other Eukaryotes - 340 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      2588854..2606892
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_100699.6 → NP_172303.5  DNA polymerase epsilon catalytic subunit [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_172303.5

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B3H4I0, F4HW04, Q9SGD5
      UniProtKB/TrEMBL
      A0A178WHZ0, A0A654E847
      Conserved Domains (4) summary
      cd05779
      Location:240 → 444
      DNA_polB_epsilon_exo; DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase epsilon, a family-B DNA polymerase
      cd05535
      Location:505 → 1122
      POLBc_epsilon; DNA polymerase type-B epsilon subfamily catalytic domain. Three DNA-dependent DNA polymerases type B (alpha, delta, and epsilon) have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase (Pol) epsilon has been proposed ...
      pfam03104
      Location:72 → 400
      DNA_pol_B_exo1; DNA polymerase family B, exonuclease domain
      pfam08490
      Location:1489 → 1872
      DUF1744; Domain of unknown function (DUF1744)

    2. NM_001331765.1 → NP_001322767.1  DNA polymerase epsilon catalytic subunit [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178WHZ0, A0A178WI12, A0A1P8AV03, A0A384L5K4
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    F4HW04.1

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