ARA7 Ras-related small GTP-binding family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: ARA7
Official Full Name: Ras-related small GTP-binding family protein
Primary source: TAIR:AT4G19640
Locus tag: AT4G19640
See related: Araport:AT4G19640
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARA-7; ARABIDOPSIS RAB GTPASE HOMOLOG F2B; ATRAB-F2B; ATRAB5B; ATRABF2B; F24J7.190; F24J7_190; RAB GTPASE HOMOLOG F2B; RAB-F2B; RABF2B
Summary: Encodes Ara7.
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Genomic context

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See ARA7 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 7

Sequence:: Chromosome: 4; NC_003075.7 (10687196..10689748, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

endosome-associated VPS9a, the conserved guanine-nucleotide exchange factor activating Rab5 GTPases, is required for both pre- and postinvasive immunity against a nonadapted powdery mildew fungus.
Title: VPS9a Activates the Rab5 GTPase ARA7 to Confer Distinct Pre- and Postinvasive Plant Innate Immunity.
VHA-a3 targets to the tonoplast through a Rab5-mediated but Rab7-independent pathway, shedding new lights on the unconventional vacuolar trafficking route in plant cells.[Rab5]
Title: Tonoplast targeting of VHA-a3 relies on a Rab5-mediated but Rab7-independent vacuolar trafficking route.
Ara7 was localized to the the extrahaustorial membrane during powdery mildew infection.
Title: Modulation of Plant RAB GTPase-Mediated Membrane Trafficking Pathway at the Interface Between Plants and Obligate Biotrophic Pathogens.
A loss of FAB1 function causes the release of late endosomal proteins, Ara7, and SNX1 from the endosome membrane, indicating that FAB1, or its product PtdIns(3,5)P2, mediates the maturation process of the late endosomes.
Title: Phosphatidylinositol 3-Phosphate 5-Kinase, FAB1/PIKfyve Kinase Mediates Endosome Maturation to Establish Endosome-Cortical Microtubule Interaction in Arabidopsis.
Arabidopsis SAND protein is not required for early-to-late endosomal maturation, although its role in mediating Rab5-to-Rab7 conversion is conserved. Instead, it is essential for the subsequent fusion of multivesicular bodies with the vacuole.
Title: Protein delivery to vacuole requires SAND protein-dependent Rab GTPase conversion for MVB-vacuole fusion.
ARA7(Q69L) overexpression induces the formation of enlarged multivesicular bodies.
Title: ARA7(Q69L) expression in transgenic Arabidopsis cells induces the formation of enlarged multivesicular bodies.
The crystal structure of ARA7-GDP-divalent calcium ion (Ca2+)-VPS9a reveals that the metal ion directly interacts with the aspartate finger of VPS9a.
Title: Direct metal recognition by guanine nucleotide-exchange factor in the initial step of the exchange reaction.
These results indicate that the two types of plant Rab5 members are functionally differentiated, even though they are regulated by the same activator, VPS9a.
Title: VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for the development of Arabidopsis thaliana.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001341361.1

Supported by ESTs:

NM_118084.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in late endosome to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular organelle	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: Ras-related small GTP-binding family protein

NP_193699.1

ARA7; FUNCTIONS IN: GTP binding; INVOLVED IN: protein transport, small GTPase mediated signal transduction; LOCATED IN: endosome, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ras GTPase (InterPro:IPR001806), Small GTP-binding protein (InterPro:IPR005225), Small GTPase (InterPro:IPR020851), Ras (InterPro:IPR013753), Ras small GTPase, Rab type (InterPro:IPR003579), Rab5-related (InterPro:IPR015599); BEST Arabidopsis thaliana protein match is: RAB homolog 1 (TAIR:AT5G45130.1); Has 26416 Blast hits to 26384 proteins in 719 species: Archae - 27; Bacteria - 133; Metazoa - 13862; Fungi - 3690; Plants - 2969; Viruses - 20; Other Eukaryotes - 5715 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

10687196..10689748 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001341360.1 → NP_001327978.1 Ras-related small GTP-binding family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8B388
NM_118084.5 → NP_193699.1 Ras-related small GTP-binding family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_193699.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9SN68

UniProtKB/TrEMBL

A0A178UXV7, A0A5S9XU39, A0A7G2FDY8

Conserved Domains (1) summary

cd01860
Location:10 → 172

Rab5_related; Rab-related GTPase family includes Rab5 and Rab22; regulates early endosome fusion
NM_001341361.1 → NP_001327977.1 Ras-related small GTP-binding family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8B381