SFC ARF GTPase-activating protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: SFC
Official Full Name: ARF GTPase-activating protein
Primary source: TAIR:AT5G13300
Locus tag: AT5G13300
See related: Araport:AT5G13300
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AGD3; ARF-GAP DOMAIN3; ASCULAR NETWORK DEFECTIVE 3; SCARFACE; T31B5.120; T31B5_120; VAN3
Summary: Belongs to 15-member small GTPase gene family, ARF-GAP domain proteins (AGD); corresponds to AGD3, and is one of four proteins belonging to class 1, together with AGD1, AGD2 and AGD4. The protein contains four domains: BAR domain, PH domain, an ARF-GAP domain, and two Ankyrin repeats. In sfc mutants, the secondary and tertiary veins of cotyledons, leaves, sepals and petals are largely replaced by small segments of discontinuous veins. sfc mutants have exaggerated responses to auxin.
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Genomic context

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See SFC in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 19

Sequence:: Chromosome: 5; NC_003076.8 (4255547..4262568, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

FKD1 and SFC co-localize with members of the RABA group of RAB-GTPases, which are proposed to act in the post-Golgi secretory pathway. The compartments labeled by FKD1 and SFC do not localize to membrane compartments induced by the fungal toxin brefeldin A.
Title: Localization of Arabidopsis FORKED1 to a RABA-positive compartment suggests a role in secretion.
Data show that ARF-GEF GNOM and ARF-GAP vascular network defective 3 (VAN3), both of which are involved in polar auxin transport-dependent morphogenesis, localize at the plasma membranes as well as in intracellular structures.
Title: ADP-ribosylation factor machinery mediates endocytosis in plant cells.
existence of PtdIns(4)P and/or IP(3)-dependent subcellular targeting and regulation of VAN3 ACAP activity that governs plant vascular tissue continuity
Title: Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants.
Scarface (SFC) is required for normal intracellular transport of PIN1 from the plasma membrane to the endosome. [Scarface] [SFC]
Title: SCARFACE encodes an ARF-GAP that is required for normal auxin efflux and vein patterning in Arabidopsis.
VAN3 is involved in vesicle trafficking and DRP1A is responsible for the vascular formation synergistically working with the VAN3 protein. [VAN3]
Title: DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis.
expression of this gene is induced by auxin and positively regulated by VAN3 itself (VAN3)
Title: VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_121333.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within leaf morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phloem or xylem histogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to auxin	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within xylem and phloem pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cellular_component	ND No biological Data available more info
located_in nucleus	ISM Inferred from Sequence Model more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in trans-Golgi network transport vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ARF GTPase-activating protein

NP_196834.3

SCARFACE (SFC); FUNCTIONS IN: ARF GTPase activator activity, phosphoinositide binding; INVOLVED IN: xylem and phloem pattern formation, response to auxin stimulus, phloem or xylem histogenesis, leaf morphogenesis; LOCATED IN: cytosol, trans-Golgi network transport vesicle, plasma membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Arf GTPase activating protein (InterPro:IPR001164), Pleckstrin homology-type (InterPro:IPR011993), Ankyrin repeat-containing domain (InterPro:IPR020683), BAR (InterPro:IPR004148), Pleckstrin homology (InterPro:IPR001849), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: ARF-GAP domain 1 (TAIR:AT5G61980.1); Has 64753 Blast hits to 27558 proteins in 1221 species: Archae - 110; Bacteria - 5131; Metazoa - 34265; Fungi - 4887; Plants - 2591; Viruses - 654; Other Eukaryotes - 17115 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

4255547..4262568 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_121333.4 → NP_196834.3 ARF GTPase-activating protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_196834.3

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WNK6, Q5W7F2, Q9LYU6

UniProtKB/TrEMBL

A0A5S9Y4E6, A0A654G1A8

Conserved Domains (7) summary

cd07606
Location:14 → 215

BAR_SFC_plant; The Bin/Amphiphysin/Rvs (BAR) domain of the plant protein SCARFACE (SFC)

cd00204
Location:711 → 816

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd13250
Location:295 → 434

PH_ACAP; ArfGAP with coiled-coil, ankyrin repeat and PH domains Pleckstrin homology (PH) domain

pfam00169
Location:293 → 430

PH; PH domain

pfam01412
Location:501 → 639

ArfGap; Putative GTPase activating protein for Arf

pfam13637
Location:729 → 782

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:729 → 759

ANK; ANK repeat [structural motif]