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    PUCHI Integrase-type DNA-binding superfamily protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 831974, updated on 18-Sep-2024

    Summary

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    Official Symbol
    PUCHI
    Official Full Name
    Integrase-type DNA-binding superfamily protein
    Primary source
    TAIR:AT5G18560
    Locus tag
    AT5G18560
    See related
    Araport:AT5G18560
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    T28N17.40; T28N17_40
    Summary
    Encodes PUCHI, a member of the ERF (ethylene response factor) subfamily B-1 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are 15 members in this subfamily including ATERF-3, ATERF-4, ATERF-7, and leafy petiole. PUCHI is required for morphogenesis in the early lateral root primordium of Arabidopsis. Expressed in early floral meristem (stage 1 to 2). Required for early floral meristem growth and for bract suppression. Triple mutant with bop1 and bop2 displays a strong defect in the determination of floral meristem identity with reduced LFY expression and the lack of AP1 expression.
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    Genomic context

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    See PUCHI in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    1
    Sequence:
    Chromosome: 5; NC_003076.8 (6164526..6166040, complement)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene protein serine/threonine kinase Neighboring gene E3 ubiquitin-protein ligase Neighboring gene Zinc finger C-x8-C-x5-C-x3-H type family protein Neighboring gene GTP1/OBG family protein Neighboring gene tonneau 2 (TON2) Neighboring gene ncRNA Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. PUCHI represses early meristem formation in developing lateral roots of Arabidopsis thaliana. Bellande K, et al. J Exp Bot, 2022 Jun 2. PMID 35224628, Free PMC Article
    2. PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation. Trinh DC, et al. Proc Natl Acad Sci U S A, 2019 Jul 9. PMID 31235573, Free PMC Article
    3. The auxin-regulated AP2/EREBP gene PUCHI is required for morphogenesis in the early lateral root primordium of Arabidopsis. Hirota A, et al. Plant Cell, 2007 Jul. PMID 17630277, Free PMC Article
    4. Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana. Goh T, et al. New Phytol, 2019 Oct. PMID 31310684
    5. DORNRÖSCHEN, DORNRÖSCHEN-LIKE, and PUCHI redundantly control floral meristem identity and organ initiation in Arabidopsis. Chandler JW, et al. J Exp Bot, 2017 Jun 15. PMID 28859377

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. PUCHI represses early meristem formation in developing lateral roots of Arabidopsis thaliana.
      Title: PUCHI represses early meristem formation in developing lateral roots of Arabidopsis thaliana.
    2. This study showed show that PUCHI expression is initiated after the first round of asymmetric cell division of lateral root (LR) founder cells and that premature induction of PUCHI during the preinitiation phase disrupts LR primordium formation.
      Title: Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana.
    3. We conclude that PUCHI-regulated very long chain fatty acid biosynthesis is part of a pathway controlling cell proliferation during lateral root and callus formation.
      Title: PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation.
    4. The combined loss of function of DORNROSCHEN, DORNROSCHEN-LIKE, and PUCHI leads to synergistic floral phenotypes, including reduced floral merosity in all whorls, which reflects redundant functions of all three genes in organ initiation rather than outgrowth.
      Title: DORNRÖSCHEN, DORNRÖSCHEN-LIKE, and PUCHI redundantly control floral meristem identity and organ initiation in Arabidopsis.
    5. PUCHI co-acts with LBD16 and LBD18 to control lateral root primordium development and lateral root emergence.
      Title: The AP2/EREBP gene PUCHI Co-Acts with LBD16/ASL18 and LBD18/ASL20 downstream of ARF7 and ARF19 to regulate lateral root development in Arabidopsis.
    6. STY1 expression in the strong drnl-2 mutant or the drn drnl-1 puchi-1 triple mutant, carrying knockdown mutations in both DRNL and its close paralogue DRN as well as one of their closest homologs, PUCHI, was significantly reduced.
      Title: Expression of Arabidopsis SHORT INTERNODES/STYLISH family genes in auxin biosynthesis zones of aerial organs is dependent on a GCC box-like regulatory element.
    7. PUCHI is required for flower meristem identity.
      Title: A role for Arabidopsis PUCHI in floral meristem identity and bract suppression.
    8. These results suggest that PUCHI acts downstream of auxin signaling and that this gene contributes to lateral root morphogenesis.[PUCHI]
      Title: The auxin-regulated AP2/EREBP gene PUCHI is required for morphogenesis in the early lateral root primordium of Arabidopsis.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables DNA-binding transcription factor activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within bract development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within bract development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within floral meristem determinacy IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within floral meristem determinacy IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within floral meristem growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within flower development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within lateral root morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of lateral root development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    Integrase-type DNA-binding superfamily protein
    NP_197357.2
    • PUCHI; CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: Integrase-type DNA-binding superfamily protein (TAIR:AT5G13910.1); Has 5489 Blast hits to 5364 proteins in 246 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 5473; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      6164526..6166040 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_121861.3 → NP_197357.2  Integrase-type DNA-binding superfamily protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_197357.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q6J9Q2
      UniProtKB/TrEMBL
      A0A178URC3, A0A5S9Y5A5, A0A654G2M9
      Conserved Domains (1) summary
      smart00380
      Location:54 → 117
      AP2; DNA-binding domain in plant proteins such as APETALA2 and EREBPs
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q6J9Q2.2

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