Synj Synaptojanin [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Synjprovided by FlyBase
Official Full Name: Synaptojaninprovided by FlyBase
Primary source: FLYBASE:FBgn0034691
Locus tag: Dmel_CG6562
See related: AllianceGenome:FB:FBgn0034691
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG6562; Dmel\CG6562; IPP; synj
Summary: Predicted to enable inositol-1,4,5-trisphosphate 5-phosphatase activity and phosphatidylinositol phosphate phosphatase activity. Involved in several processes, including maintenance of presynaptic active zone structure; positive regulation of autophagosome assembly; and synaptic vesicle endocytosis. Located in type I terminal bouton; vacuole; and vesicle membrane. Is expressed in several structures, including adult head; embryonic/larval gut; embryonic/larval salivary gland; eye-antennal disc; and larval central nervous system. Used to study Parkinson's disease. Human ortholog(s) of this gene implicated in Parkinson's disease; Parkinson's disease 20; and developmental and epileptic encephalopathy 53. Orthologous to several human genes including SYNJ1 (synaptojanin 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 58B4-58B4; 2-98 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (22080176..22085767)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (17967681..17973272)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Minibrain kinase and calcineurin coordinate activity-dependent bulk endocytosis through synaptojanin.
Title: Minibrain kinase and calcineurin coordinate activity-dependent bulk endocytosis through synaptojanin.
This is the first report that identifies synaptojanin as a negative regulator of Hh signaling, implying its importance and an additional regulatory step in Hh signal transduction.
Title: Synaptojanin regulates Hedgehog signalling by modulating phosphatidylinositol 4-phosphate levels.
This study demonstrated that Synaptojanin, a protein with a known role in synaptic vesicle endocytosis, is phosphorylated at S1029 in vivo by the Minibrain kinase. We further demonstrate that the phosphorylation status of Synaptojanin at S1029 differentially regulates its participation in the recycling of distinct synaptic vesicle pools.
Title: Phosphorylation of Synaptojanin Differentially Regulates Endocytosis of Functionally Distinct Synaptic Vesicle Pools.
Mnb is a synaptic kinase that promotes efficient synaptic vesicle recycling by Synj function at the neuromuscular junction.
Title: Activity-dependent facilitation of Synaptojanin and synaptic vesicle recycling by the Minibrain kinase.
Findings imply that restoring the level of any one of these genes dap160/itsn1, synj/synj1, and nla/dscr1 may reduce endocytic defects seen in DS.
Title: Upregulation of three Drosophila homologs of human chromosome 21 genes alters synaptic function: implications for Down syndrome.
Synjanin is mislocalized and destabilized at synapses devoid of Endophilin, suggesting that Endophilin recruits and stabilizes Synjanin on newly formed vesicles to promote vesicle uncoating.
Title: Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating.
In the absence of synaptojanin or endophilin, vesicles undergo full fusion and re-formation.
Title: A slowed classical pathway rather than kiss-and-run mediates endocytosis at synapses lacking synaptojanin and endophilin.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables inositol-1,4,5-trisphosphate 5-phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phosphate 5-phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables phosphatidylinositol-3-phosphate phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables phosphatidylinositol-4-phosphate phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in bulk synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clathrin-dependent synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of presynaptic active zone structure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylinositol dephosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synapse structure or activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in autolysosome	IDA Inferred from Direct Assay more info	PubMed
located_in autophagosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed
located_in type I terminal bouton	IDA Inferred from Direct Assay more info	PubMed
located_in vesicle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: synaptojanin

Names: CG6562-PA; CG6562-PB; CG6562-PC; Synj-PA; Synj-PB; Synj-PC

NP_001246454.1

EC 3.1.3.-

EC 3.1.3.36

EC 3.1.3.64

NP_569729.1

EC 3.1.3.-

EC 3.1.3.36

EC 3.1.3.64

NP_726155.1

EC 3.1.3.-

EC 3.1.3.36

EC 3.1.3.64

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

22080176..22085767

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_130462.4 → NP_569729.1 synaptojanin, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_569729.1

Status: REVIEWED

UniProtKB/TrEMBL

Q5U0V7

Related

FBpp0071670

Conserved Domains (3) summary

cd09089
Location:538 → 863

INPP5c_Synj; Catalytic inositol polyphosphate 5-phosphatase (INPP5c) domain of synaptojanins

pfam02383
Location:61 → 353

Syja_N; SacI homology domain

pfam08952
Location:862 → 1008

DUF1866; Domain of unknown function (DUF1866)
NM_166505.2 → NP_726155.1 synaptojanin, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726155.1

Status: REVIEWED

UniProtKB/TrEMBL

Q5U0V7

Related

FBpp0071671

Conserved Domains (3) summary

cd09089
Location:538 → 863

INPP5c_Synj; Catalytic inositol polyphosphate 5-phosphatase (INPP5c) domain of synaptojanins

pfam02383
Location:61 → 353

Syja_N; SacI homology domain

pfam08952
Location:862 → 1008

DUF1866; Domain of unknown function (DUF1866)
NM_001259525.1 → NP_001246454.1 synaptojanin, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246454.1

Status: REVIEWED

UniProtKB/TrEMBL

Q5U0V7

Conserved Domains (3) summary

cd09089
Location:538 → 863

INPP5c_Synj; Catalytic inositol polyphosphate 5-phosphatase (INPP5c) domain of synaptojanins

pfam02383
Location:61 → 353

Syja_N; SacI homology domain

pfam08952
Location:862 → 1008

DUF1866; Domain of unknown function (DUF1866)