Necab2 N-terminal EF-hand calcium binding protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Necab2provided by MGI
Official Full Name: N-terminal EF-hand calcium binding protein 2provided by MGI
Primary source: MGI:MGI:2152211
See related: Ensembl:ENSMUSG00000031837 AllianceGenome:MGI:2152211
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Efcbp2
Summary: Predicted to enable A2A adenosine receptor binding activity; identical protein binding activity; and type 5 metabotropic glutamate receptor binding activity. Predicted to be involved in positive regulation of protein localization to membrane; regulation of amyloid precursor protein biosynthetic process; and regulation of signal transduction. Predicted to be located in plasma membrane. Predicted to be active in cytoplasm. Is expressed in brain; embryo ectoderm; eye; nasal pit; and trigeminal ganglion. Orthologous to human NECAB2 (N-terminal EF-hand calcium binding protein 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in frontal lobe adult (RPKM 6.6), CNS E18 (RPKM 5.5) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 E1; 8 67.73 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (120173431..120199379)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (119446719..119472640)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We identified over 100 proteins with robustly enriched expression in area CA2 compared to CA1. Many of these proteins, including RGS14 and NECAB2, have already been shown to be enriched in CA2 and important for its function, while many more merit further study in the context of enhanced expression in this enigmatic brain region.
Title: Specific Proteomes of Hippocampal Regions CA2 and CA1 Reveal Proteins Linked to the Unique Physiology of Area CA2.
NECAB2 was downregulated by peripheral nerve injury, leading to the hypothesis that NECAB2 loss of function could limit pain sensation. Indeed, Necab2-/- mice reached a pain-free state significantly faster after peripheral inflammation than did WT littermates.
Title: Ca2+-binding protein NECAB2 facilitates inflammatory pain hypersensitivity.
The genes for the three Necab's, including Necab-2, were expressed in specific, non-overlapping areas of the hippocampus.
Title: Expression of the calcium binding proteins Necab-1,-2 and -3 in the adult mouse hippocampus and dentate gyrus.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:487629

BB446373 (e-PCR)
- UniSTS:208335

ha2601 (e-PCR)
- UniSTS:515571

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables A2A adenosine receptor binding	IEA Inferred from Electronic Annotation more info
enables A2A adenosine receptor binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables type 5 metabotropic glutamate receptor binding	IEA Inferred from Electronic Annotation more info
enables type 5 metabotropic glutamate receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in negative regulation of G protein-coupled receptor internalization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of G protein-coupled receptor internalization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of adenosine receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of adenosine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glutamate receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of glutamate receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to membrane	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein localization to membrane	ISO Inferred from Sequence Orthology more info
involved_in regulation of amyloid precursor protein biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in axon	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in dendrite	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in postsynapse	IEA Inferred from Electronic Annotation more info
located_in presynapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: N-terminal EF-hand calcium-binding protein 2

Names: EF hand calcium binding protein 2; neuronal calcium binding 2; neuronal calcium-binding protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_054095.2 → NP_473436.1 N-terminal EF-hand calcium-binding protein 2

See identical proteins and their annotated locations for NP_473436.1

Status: PROVISIONAL

Source sequence(s)

AC166781

Consensus CDS

CCDS22705.1

UniProtKB/Swiss-Prot

Q1LZI4, Q8C492, Q91ZP9

UniProtKB/TrEMBL

Q3B7X9

Related

ENSMUSP00000095966.4, ENSMUST00000098363.10

Conserved Domains (3) summary

cd00051
Location:68 → 130

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam03992
Location:291 → 361

ABM; Antibiotic biosynthesis monooxygenase

pfam13499
Location:68 → 126

EF-hand_7; EF-hand domain pair

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

120173431..120199379

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036153728.1 → XP_036009621.1 N-terminal EF-hand calcium-binding protein 2 isoform X2

Conserved Domains (1) summary

pfam03992
Location:162 → 232

ABM; Antibiotic biosynthesis monooxygenase
XM_017312526.3 → XP_017168015.1 N-terminal EF-hand calcium-binding protein 2 isoform X2

UniProtKB/Swiss-Prot

Q91ZP9

Conserved Domains (1) summary

pfam03992
Location:162 → 232

ABM; Antibiotic biosynthesis monooxygenase
XM_036153727.1 → XP_036009620.1 N-terminal EF-hand calcium-binding protein 2 isoform X1

Conserved Domains (1) summary

pfam03992
Location:179 → 249

ABM; Antibiotic biosynthesis monooxygenase