Dld dihydrolipoamide dehydrogenase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dldprovided by RGD
Official Full Name: dihydrolipoamide dehydrogenaseprovided by RGD
Primary source: RGD:735073
See related: EnsemblRapid:ENSRNOG00000006364 AllianceGenome:RGD:735073
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease. [provided by RefSeq, Jan 2014]
Expression: Biased expression in Heart (RPKM 1345.2), Muscle (RPKM 871.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 6q16

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (53631686..53655059, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (47904153..47924814, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (50597677..50618694, complement)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results, together with the findings that the protein contents of DLDH, sirt3, and NQO1 all are decreased in the diabetic lung, demonstrate that redox imbalance, mitochondrial abnormality, and oxidative stress contribute to lung injury in diabetes.
Title: Redox imbalance and mitochondrial abnormalities in the diabetic lung.
DLD is down-regulation and related to steroid biosynthesis in Leyig cells exposed to heavy metals; cAMP/PKA act as downstream effector molecules of DLD, which activate phosphorylation of ERK1/2 to initiate the steroidogenesis.
Title: Cytotoxic mechanism related to dihydrolipoamide dehydrogenase in Leydig cells exposed to heavy metals.
Density of NADHd-positive myoenteric neurons increased in experimental diabetes.
Title: Effects of insulin treatment on HuC/HuD, NADH diaphorase, and nNOS-positive myoenteric neurons of the duodenum of adult rats with acute diabetes.
Changes in Dld expression and activity during postnatal development and aging are reported.
Title: Changes in dihydrolipoamide dehydrogenase expression and activity during postnatal development and aging in the rat brain.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH127918 (e-PCR)
- UniSTS:211228

RH127725 (e-PCR)
- UniSTS:211035

RH130917 (e-PCR)
- UniSTS:214201

RH143660 (e-PCR)
- UniSTS:232906

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables NAD binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to branched-chain alpha-keto acid dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables dihydrolipoyl dehydrogenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dihydrolipoyl dehydrogenase activity	IDA Inferred from Direct Assay more info	PubMed
enables dihydrolipoyl dehydrogenase activity	IEA Inferred from Electronic Annotation more info
enables dihydrolipoyl dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables lipoamide binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to pyruvate dehydrogenase (NAD+) activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in 2-oxoglutarate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in 2-oxoglutarate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in acetyl-CoA biosynthetic process from pyruvate	IDA Inferred from Direct Assay more info	PubMed
involved_in acetyl-CoA biosynthetic process from pyruvate	ISO Inferred from Sequence Orthology more info
involved_in branched-chain amino acid catabolic process	ISO Inferred from Sequence Orthology more info
involved_in dihydrolipoamide metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within gastrulation	ISO Inferred from Sequence Orthology more info
involved_in lipoate metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within mitochondrial electron transport, NADH to ubiquinone	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within proteolysis	ISO Inferred from Sequence Orthology more info
involved_in pyruvate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of membrane potential	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sperm capacitation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of acetyltransferase complex	ISO Inferred from Sequence Orthology more info
located_in acrosomal matrix	ISO Inferred from Sequence Orthology more info
located_in acrosomal vesicle	IEA Inferred from Electronic Annotation more info
part_of branched-chain alpha-ketoacid dehydrogenase complex	ISO Inferred from Sequence Orthology more info
located_in cilium	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in motile cilium	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of oxoadipate dehydrogenase complex	ISO Inferred from Sequence Orthology more info
part_of oxoglutarate dehydrogenase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of oxoglutarate dehydrogenase complex	IDA Inferred from Direct Assay more info	PubMed
part_of oxoglutarate dehydrogenase complex	ISO Inferred from Sequence Orthology more info
part_of pyruvate dehydrogenase complex	IDA Inferred from Direct Assay more info	PubMed
part_of pyruvate dehydrogenase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dihydrolipoyl dehydrogenase, mitochondrial

Names: dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)

NP_955417.1

EC 1.8.1.4

XP_008762812.1

EC 1.8.1.4

XP_038967889.1

EC 1.8.1.4

XP_063117729.1

EC 1.8.1.4

XP_063117730.1

EC 1.8.1.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_199385.2 → NP_955417.1 dihydrolipoyl dehydrogenase, mitochondrial precursor

See identical proteins and their annotated locations for NP_955417.1

Status: VALIDATED

Source sequence(s)

FM044626, FM046215, FM087994, FM093110, FM100976, FM139628

UniProtKB/Swiss-Prot

Q6P6R2

UniProtKB/TrEMBL

A0A8L2Q3R1, A6HB44

Related

ENSRNOP00000008980.6, ENSRNOT00000008980.8

Conserved Domains (1) summary

TIGR01350
Location:43 → 508

lipoamide_DH; dihydrolipoamide dehydrogenase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086024.1 Reference GRCr8

Range

53631686..53655059 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063261659.1 → XP_063117729.1 dihydrolipoyl dehydrogenase, mitochondrial isoform X1

UniProtKB/Swiss-Prot

Q6P6R2

UniProtKB/TrEMBL

A6HB44
XM_039111961.1 → XP_038967889.1 dihydrolipoyl dehydrogenase, mitochondrial isoform X3

UniProtKB/TrEMBL

A0A8I5ZXS2, A0A8L2Q3R1

Conserved Domains (1) summary

cl39093
Location:36 → 501

Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase
XM_008764590.4 → XP_008762812.1 dihydrolipoyl dehydrogenase, mitochondrial isoform X2

UniProtKB/TrEMBL

A0A8L2Q3R1

Related

ENSRNOP00000083816.2, ENSRNOT00000103902.2

Conserved Domains (4) summary

TIGR01350
Location:41 → 506

lipoamide_DH; dihydrolipoamide dehydrogenase

pfam00070
Location:213 → 286

Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase

pfam02852
Location:388 → 494

Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerization domain

cl21454
Location:43 → 220

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins
XM_063261660.1 → XP_063117730.1 dihydrolipoyl dehydrogenase, mitochondrial isoform X4