Vamp8 vesicle-associated membrane protein 8 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Vamp8provided by RGD
Official Full Name: vesicle-associated membrane protein 8provided by RGD
Primary source: RGD:620421
See related: EnsemblRapid:ENSRNOG00000012748 AllianceGenome:RGD:620421
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables SNAP receptor activity and syntaxin binding activity. Involved in protein-containing complex assembly and vesicle fusion. Located in endosome; lysosomal membrane; and midbody. Part of SNARE complex. Orthologous to human VAMP8 (vesicle associated membrane protein 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Lung (RPKM 1046.1), Kidney (RPKM 774.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q32

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (106000570..106011081, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (104442383..104452884, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (100250301..100252756, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

acute inhibition of VAMP8-mediated secretion during pancreatitis triggers intracellular trypsin accumulation and loss of the early endosomal compartment
Title: Acute acinar pancreatitis blocks vesicle-associated membrane protein 8 (VAMP8)-dependent secretion, resulting in intracellular trypsin accumulation.
The authors took a proteomics approach using a PKCB knockout mast cell model and found that the key mast cell secretory protein VAMP8 becomes phosphorylated by PKC at multiple residues in the SNARE domain.
Title: Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.
VAMP8, which also mediates fusion of late endosomes and lysosomes, plays a major role in hexosaminidase release
Title: Endobrevin/VAMP8 mediates exocytotic release of hexosaminidase from rat basophilic leukaemia cells.
Individual pancreatic acinar cells express VAMP 8-specific populations of zymogen granules that orchestrate the constitutive and calcium(2+)-regulated secretory pathways.
Title: Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs
Title: Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables SNAP receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables SNAP receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables chloride channel inhibitor activity	IEA Inferred from Electronic Annotation more info
enables chloride channel inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in SNARE complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in SNARE complex assembly	ISO Inferred from Sequence Orthology more info
involved_in autophagosome maturation	ISO Inferred from Sequence Orthology more info
involved_in autophagosome maturation	ISS Inferred from Sequence or Structural Similarity more info
involved_in autophagosome membrane docking	IEA Inferred from Electronic Annotation more info
involved_in autophagosome membrane docking	ISO Inferred from Sequence Orthology more info
involved_in autophagy	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in defense response to virus	IEA Inferred from Electronic Annotation more info
NOT involved_in eosinophil degranulation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of localization in cell	ISO Inferred from Sequence Orthology more info
involved_in membrane fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in mucus secretion	IBA Inferred from Biological aspect of Ancestor more info
involved_in mucus secretion	IEA Inferred from Electronic Annotation more info
involved_in mucus secretion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of secretion by cell	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of secretion by cell	ISO Inferred from Sequence Orthology more info
NOT involved_in neutrophil degranulation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of histamine secretion by mast cell	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of histamine secretion by mast cell	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of pancreatic amylase secretion	ISO Inferred from Sequence Orthology more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein-containing complex assembly	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of endocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein localization to plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in symbiont entry into host cell	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within symbiont entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in vesicle fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle fusion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within vesicle fusion	ISO Inferred from Sequence Orthology more info
involved_in vesicle-mediated transport	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within zymogen granule exocytosis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of SNARE complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SNARE complex	IDA Inferred from Direct Assay more info	PubMed
part_of SNARE complex	IEA Inferred from Electronic Annotation more info
part_of SNARE complex	ISO Inferred from Sequence Orthology more info
located_in azurophil granule membrane	IEA Inferred from Electronic Annotation more info
located_in azurophil granule membrane	ISO Inferred from Sequence Orthology more info
located_in basal part of cell	ISO Inferred from Sequence Orthology more info
located_in basolateral part of cell	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
located_in late endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome membrane	IEA Inferred from Electronic Annotation more info
located_in late endosome membrane	ISO Inferred from Sequence Orthology more info
located_in lysosomal membrane	IDA Inferred from Direct Assay more info	PubMed
located_in lysosomal membrane	IEA Inferred from Electronic Annotation more info
located_in lysosomal membrane	ISO Inferred from Sequence Orthology more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
is_active_in mucin granule	IBA Inferred from Biological aspect of Ancestor more info
located_in mucin granule	IEA Inferred from Electronic Annotation more info
located_in mucin granule	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in phagocytic vesicle	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed
located_in recycling endosome	IEA Inferred from Electronic Annotation more info
located_in recycling endosome	ISO Inferred from Sequence Orthology more info
located_in secretory granule	ISO Inferred from Sequence Orthology more info
located_in secretory granule membrane	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISO Inferred from Sequence Orthology more info
located_in zymogen granule	ISO Inferred from Sequence Orthology more info
located_in zymogen granule membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: vesicle-associated membrane protein 8

Names: EDB; VAMP-8; endobrevin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031827.2 → NP_114015.1 vesicle-associated membrane protein 8

See identical proteins and their annotated locations for NP_114015.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000004

UniProtKB/Swiss-Prot

Q9WUF4

UniProtKB/TrEMBL

A0A8I6AB54, A6IAB7

Related

ENSRNOP00000083978.1, ENSRNOT00000096938.2

Conserved Domains (2) summary

cd15868
Location:9 → 75

R-SNARE_VAMP8; SNARE motif of VAMP8

pfam00957
Location:8 → 75

Synaptobrevin; Synaptobrevin

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

106000570..106011081 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039108449.2 → XP_038964377.1 vesicle-associated membrane protein 8 isoform X1

UniProtKB/TrEMBL

A0A8I6AB54, F1LNT8

Related

ENSRNOP00000017301.3, ENSRNOT00000017301.6

Conserved Domains (1) summary

cd15868
Location:25 → 91

R-SNARE_VAMP8; SNARE motif of VAMP8