Spdef SAM pointed domain containing ets transcription factor [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spdefprovided by MGI
Official Full Name: SAM pointed domain containing ets transcription factorprovided by MGI
Primary source: MGI:MGI:1353422
See related: Ensembl:ENSMUSG00000024215 AllianceGenome:MGI:1353422
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pse; PDEF
Summary: Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Acts upstream of or within several processes, including columnar/cuboidal epithelial cell differentiation; positive regulation of cell fate commitment; and regulation of transcription by RNA polymerase II. Located in nucleus. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and sensory organ. Orthologous to human SPDEF (SAM pointed domain containing ETS transcription factor). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in colon adult (RPKM 65.2), stomach adult (RPKM 24.2) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 14.59 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (27933416..27949977, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (27714446..27731003, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lactobacillus rhamnosus 76 alleviates airway inflammation in ovalbumin-allergic mice and improves mucus secretion by down-regulating STAT6/SPDEF pathway.
Title: Lactobacillus rhamnosus 76 alleviates airway inflammation in ovalbumin-allergic mice and improves mucus secretion by down-regulating STAT6/SPDEF pathway.
that Spdef regulates baseline Muc5b expression in respiratory epithelia but does not contribute to Muc5b regulation in a mouse model of Muc5b-predominant mucus obstruction caused by airway dehydration
Title: Role of Spdef in the Regulation of Muc5b Expression in the Airways of Naive and Mucoobstructed Mice.
a novel mechanism that contributes to the EMT and metastatic phenotype observed in a subset of ADT-resistant prostate cancer, where the CCL2 is stimulated through the inactivated of AR-mediated SPDEF.
Title: Androgen deprivation therapy-induced epithelial-mesenchymal transition of prostate cancer through downregulating SPDEF and activating CCL2.
In studies of mice and human CRC, we found that SPDEF induces a quiescent state in CRC cells by disrupting binding of b-catenin to TCF1 and TCF3 and regulation of genes that control the cell cycle.
Title: SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of β-catenin.
SPDEF causes goblet cell differentiation and Th2 inflammation during postnatal development and is required for goblet cell metaplasia and normal Th2 inflammatory responses to house dust mite aeroallergen.
Title: Airway epithelial SPDEF integrates goblet cell differentiation and pulmonary Th2 inflammation.
SPDEF inhibits prostate carcinogenesis by disrupting a positive feedback loop in regulation of the Foxm1 oncogene.
Title: SPDEF inhibits prostate carcinogenesis by disrupting a positive feedback loop in regulation of the Foxm1 oncogene.
Spdef(-/-) mice have an ocular surface phenotype similar to that in moderate dry eye, providing a new, more convenient model for the disease.
Title: Spdef null mice lack conjunctival goblet cells and provide a model of dry eye.
SPDEF inhibited the expression of beta-catenin-target genes in mouse colon tumors, and interacted with beta-catenin to block its transcriptional activity in CRC cell lines, resulting in lower levels of cyclin D1 and c-MYC.
Title: SPDEF functions as a colorectal tumor suppressor by inhibiting β-catenin activity.
Our data reveal a novel nonredundant role for Serpinb3a in mediating mucus production through regulation of sterile alpha motif pointed domain containing v-ets avian erythroblastosis virus E26 oncogene homolog transcription factor expression
Title: A nonredundant role for mouse Serpinb3a in the induction of mucus production in asthma.
Spdef functions in secretory intestinal cells and suggest a common molecular mechanism for maturation of gastrointestinal secretory lineages.
Title: Requirement of the epithelium-specific Ets transcription factor Spdef for mucous gland cell function in the gastric antrum.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MARC_21610-21611:1033065458:1 (e-PCR)
- UniSTS:268587

Spdef (e-PCR), detects polymorphism
- UniSTS:525274

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of epithelial cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glandular epithelial cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intestinal epithelial cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lung goblet cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: SAM pointed domain-containing Ets transcription factor

Names: prostate epithelium-specific Ets transcription factor; prostate specific ets transcription factor; prostate-derived Ets factor; prostate-specific Ets

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357728.1 → NP_001344657.1 SAM pointed domain-containing Ets transcription factor

Status: VALIDATED

Source sequence(s)

AC131800, BY446800

Consensus CDS

CCDS28568.1

UniProtKB/Swiss-Prot

Q9WTP3

UniProtKB/TrEMBL

A0A3F2YNL9

Conserved Domains (2) summary

cd08532
Location:124 → 204

SAM_PNT-PDEF-like; Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor

smart00413
Location:238 → 324

ETS; erythroblast transformation specific domain
NM_001414277.1 → NP_001401206.1 SAM pointed domain-containing Ets transcription factor

Status: VALIDATED

Source sequence(s)

AC131800

UniProtKB/Swiss-Prot

Q9WTP3

UniProtKB/TrEMBL

A0A3F2YNL9
NM_001414278.1 → NP_001401207.1 SAM pointed domain-containing Ets transcription factor

Status: VALIDATED

Source sequence(s)

AC131800

UniProtKB/Swiss-Prot

Q9WTP3

UniProtKB/TrEMBL

A0A3F2YNL9
NM_013891.4 → NP_038919.1 SAM pointed domain-containing Ets transcription factor

See identical proteins and their annotated locations for NP_038919.1

Status: VALIDATED

Source sequence(s)

AK078863, AV370101, AV370994

Consensus CDS

CCDS28568.1

UniProtKB/Swiss-Prot

Q9WTP3

UniProtKB/TrEMBL

A0A3F2YNL9

Related

ENSMUSP00000025054.3, ENSMUST00000025054.10

Conserved Domains (2) summary

cd08532
Location:124 → 204

SAM_PNT-PDEF-like; Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor

smart00413
Location:238 → 324

ETS; erythroblast transformation specific domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

27933416..27949977 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006524380.5 → XP_006524443.1 SAM pointed domain-containing Ets transcription factor isoform X1

See identical proteins and their annotated locations for XP_006524443.1

UniProtKB/Swiss-Prot

Q9WTP3

UniProtKB/TrEMBL

A0A3F2YNL9

Conserved Domains (2) summary

cd08532
Location:124 → 204

SAM_PNT-PDEF-like; Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor

smart00413
Location:238 → 324

ETS; erythroblast transformation specific domain
XM_006524379.5 → XP_006524442.1 SAM pointed domain-containing Ets transcription factor isoform X1

See identical proteins and their annotated locations for XP_006524442.1

UniProtKB/Swiss-Prot

Q9WTP3

UniProtKB/TrEMBL

A0A3F2YNL9

Conserved Domains (2) summary

cd08532
Location:124 → 204

SAM_PNT-PDEF-like; Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor

smart00413
Location:238 → 324

ETS; erythroblast transformation specific domain
XM_006524381.5 → XP_006524444.1 SAM pointed domain-containing Ets transcription factor isoform X1

See identical proteins and their annotated locations for XP_006524444.1

UniProtKB/Swiss-Prot

Q9WTP3

UniProtKB/TrEMBL

A0A3F2YNL9

Conserved Domains (2) summary

cd08532
Location:124 → 204

SAM_PNT-PDEF-like; Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor

smart00413
Location:238 → 324

ETS; erythroblast transformation specific domain