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    Mid2 midline 2 [ Mus musculus (house mouse) ]

    Gene ID: 23947, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Mid2provided by MGI
    Official Full Name
    midline 2provided by MGI
    Primary source
    MGI:MGI:1344333
    See related
    Ensembl:ENSMUSG00000000266 AllianceGenome:MGI:1344333
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    FXY2; Trim1
    Summary
    Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and transcription coactivator activity. Predicted to be involved in several processes, including defense response to symbiont; negative regulation of viral transcription; and positive regulation of NF-kappaB transcription factor activity. Predicted to be located in cytoplasm. Predicted to colocalize with microtubule. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; heart; and sensory organ. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 101. Orthologous to human MID2 (midline 2). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Ubiquitous expression in lung adult (RPKM 6.4), bladder adult (RPKM 4.0) and 26 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Mid2 in Genome Data Viewer
    Location:
    X F1; X 61.35 cM
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (139565326..139668464)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (140664304..140767715)

    Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene TSC22 domain family, member 3 Neighboring gene glycine cleavage system protein H (aminomethyl carrier) pseudogene Neighboring gene predicted gene, 23199 Neighboring gene eukaryotic translation initiation factor 2C, 5 Neighboring gene eukaryotic translation initiation factor 2C, 2 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells. Boding L, et al. APMIS, 2015 Aug. PMID 25924778
    2. Mid1/Mid2 expression in craniofacial development and a literature review of X-linked opitz syndrome. Li B, et al. Mol Genet Genomic Med, 2016 Jan. PMID 26788540, Free PMC Article
    3. FXY2/MID2, a gene related to the X-linked Opitz syndrome gene FXY/MID1, maps to Xq22 and encodes a FNIII domain-containing protein that associates with microtubules. Perry J, et al. Genomics, 1999 Dec 15. PMID 10644436
    4. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Buchner G, et al. Hum Mol Genet, 1999 Aug. PMID 10400986
    5. The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote Cell Division. Gholkar AA, et al. Cell Rep, 2016 Jan 12. PMID 26748699, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (1) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables enzyme binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables microtubule binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables microtubule binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables phosphoprotein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transcription coactivator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in innate immune response ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of viral entry into host cell ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of viral transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of DNA-binding transcription factor activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of NF-kappaB transcription factor activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of autophagy ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of autophagy ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in positive regulation of canonical NF-kappaB signal transduction ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization to microtubule IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein localization to microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in suppression of viral release by host ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    colocalizes_with microtubule IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in microtubule IEA
    Inferred from Electronic Annotation
    more info
    		  
    colocalizes_with microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    probable E3 ubiquitin-protein ligase MID2
    Names
    RING-type E3 ubiquitin transferase MID2
    midline defect 2
    tripartite motif-containing protein 1
    NP_001345295.1
    NP_001345296.1
    NP_001390295.1
    NP_035975.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001358366.2 → NP_001345295.1  probable E3 ubiquitin-protein ligase MID2 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL683809, BX470203
      UniProtKB/Swiss-Prot
      Q9QUS6
      UniProtKB/TrEMBL
      B1AVF5
      Related
      ENSMUSP00000108614.2, ENSMUST00000112990.8
      Conserved Domains (5) summary
      smart00502
      Location:239 → 364
      BBC; B-Box C-terminal domain
      smart00336
      Location:195 → 232
      BBOX; B-Box-type zinc finger
      cd00063
      Location:401 → 501
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd13739
      Location:506 → 675
      SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
      cd16754
      Location:30 → 82
      RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins

    2. NM_001358367.2 → NP_001345296.1  probable E3 ubiquitin-protein ligase MID2 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AL683809, BX470203
      UniProtKB/TrEMBL
      B1AVF5
      Related
      ENSMUSP00000108612.2, ENSMUST00000112988.8
      Conserved Domains (5) summary
      smart00502
      Location:239 → 364
      BBC; B-Box C-terminal domain
      smart00336
      Location:195 → 232
      BBOX; B-Box-type zinc finger
      cd00063
      Location:401 → 531
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd13739
      Location:536 → 705
      SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
      cd16754
      Location:30 → 82
      RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins

    3. NM_001403366.1 → NP_001390295.1  probable E3 ubiquitin-protein ligase MID2 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AL683809, BX470203
      UniProtKB/TrEMBL
      B1AVF5

    4. NM_011845.4 → NP_035975.1  probable E3 ubiquitin-protein ligase MID2 isoform 3

      See identical proteins and their annotated locations for NP_035975.1

      Status: VALIDATED

      Source sequence(s)
      AL683809, BX470203
      Consensus CDS
      CCDS41151.1
      UniProtKB/TrEMBL
      B1AVF4, B1AVF5
      Related
      ENSMUSP00000108617.2, ENSMUST00000112993.2
      Conserved Domains (7) summary
      smart00502
      Location:219 → 344
      BBC; B-Box C-terminal domain
      cd00063
      Location:381 → 481
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      cd13739
      Location:486 → 655
      SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
      cd16754
      Location:10 → 62
      RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins
      pfam18568
      Location:323 → 374
      COS; TRIM C-terminal subgroup One Signature domain
      cd19823
      Location:173 → 212
      Bbox2_MID2_C-I; B-box-type 2 zinc finger found in midline-2 (MID2) and similar proteins
      cd19837
      Location:115 → 167
      Bbox1_MID2_C-I; B-box-type 1 zinc finger found in midline-2 (MID2) and similar proteins

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000086.8 Reference GRCm39 C57BL/6J

      Range
      139565326..139668464
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9QUS6.2 GenPept UniProtKB/Swiss-Prot:Q9QUS6

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