Mylk3 myosin light chain kinase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mylk3provided by MGI
Official Full Name: myosin light chain kinase 3provided by MGI
Primary source: MGI:MGI:2443063
See related: Ensembl:ENSMUSG00000031698 AllianceGenome:MGI:2443063
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MLCK; D830007F02Rik
Summary: Enables calcium/calmodulin-dependent protein kinase activity and myosin light chain kinase activity. Involved in protein phosphorylation and sarcomere organization. Located in cytoplasm. Colocalizes with actin cytoskeleton. Is expressed in heart atrium; heart ventricle; inner ear; liver; and tibialis anterior. Orthologous to human MYLK3 (myosin light chain kinase 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in heart adult (RPKM 54.9), liver E14 (RPKM 12.4) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C3; 8 41.61 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (86050933..86112969, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (85324928..85386340, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin.
Title: Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin.
A temporal Ca(2+) desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKbeta/PP2A pathways.
Title: A temporal Ca(2+) desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKβ/PP2A pathways.
Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not.
Title: Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not.
Pharmacological study revealed that lamellipodia formation mediated by arp2/3 and contractility regulated by myosin light chain kinase (MLCK) were responsible for the intriguing turning behavior of T cells climbing the ramp-like structures.
Title: Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation.
Acanthopanax senticosus polysaccharide pretreatment may be associated with inhibition of the NF-kappaB/MLCK pathway and concomitant amelioration of LPS-induced tight junction dysfunction of intestinal epithelium in endotoxemia
Title: Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model.
that MLCK is competing with centrally active ROCK for a limiting pool of monomer with which to drive lamellar filament assembly
Title: Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.
This study demonstrated that catchlike property during dynamic performance in mouse hindlimb muscles with (wild-type) and without MLCK(-/-) ) the primary mechanism for PTP (myosin phosphorylation).
Title: Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle.
Ablation of cardiac Mylk3 suggests that Mylk3 plays a pivotal role in the transition from compensated to decompensated hypertrophy via cardiac myocyte atrophy and sarcomeric disorganization.
Title: Acute heart failure with cardiomyocyte atrophy induced in adult mice by ablation of cardiac myosin light chain kinase.
Data suggest that galectin-3 in hepatocellular carcinoma cells promotes cell migration by inducing RhoA GTPase activity, activating Mlck, up-regulating phosphorylation of MLC2 (myosin light chain 2), and inducing actin rearrangement.
Title: Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation.
epithelial MLCK-activated brush border fanning by IFN-gamma promotes adherence and internalization of normally noninvasive enteric bacteria
Title: Commensal bacterial endocytosis in epithelial cells is dependent on myosin light chain kinase-activated brush border fanning by interferon-γ.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Spontaneous (1) 1 citation
Targeted (4) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
NOT enables calcium/calmodulin-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium/calmodulin-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium/calmodulin-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables myosin light chain kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables myosin light chain kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables myosin light chain kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cardiac myofibril assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in cardiac myofibril assembly	IEA Inferred from Electronic Annotation more info
involved_in cardiac myofibril assembly	ISO Inferred from Sequence Orthology more info
involved_in cellular response to interleukin-1	IEA Inferred from Electronic Annotation more info
involved_in cellular response to interleukin-1	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of membrane permeability	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of sarcomere organization	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of sarcomere organization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of tight junction disassembly	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of vascular permeability involved in acute inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in sarcomere organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in sarcomere organization	IDA Inferred from Direct Assay more info	PubMed
involved_in sarcomere organization	ISO Inferred from Sequence Orthology more info
involved_in sarcomerogenesis	IEA Inferred from Electronic Annotation more info
involved_in sarcomerogenesis	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
colocalizes_with actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: myosin light chain kinase 3

Names: cardiac MLCK; cardiac-MyBP-C-associated Ca/CaM kinase; cardiac-specific myosin light chain kinase; putative myosin light chain kinase 3

NP_001284541.1

EC 2.7.11.18

NP_780650.2

EC 2.7.11.18

XP_006530888.1

EC 2.7.11.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001297612.1 → NP_001284541.1 myosin light chain kinase 3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

AK146683, AK165216, BB843262

Consensus CDS

CCDS80910.1

UniProtKB/TrEMBL

D3Z630

Related

ENSMUSP00000113389.2, ENSMUST00000122452.8

Conserved Domains (2) summary

smart00220
Location:361 → 613

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14192
Location:353 → 613

STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3
NM_175441.5 → NP_780650.2 myosin light chain kinase 3 isoform 1

See identical proteins and their annotated locations for NP_780650.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC134854

Consensus CDS

CCDS40422.1

UniProtKB/Swiss-Prot

B0LY41, Q3UIZ8, Q3V3V0, Q8BWD1

UniProtKB/TrEMBL

B2RQL6

Related

ENSMUSP00000034133.8, ENSMUST00000034133.14

Conserved Domains (2) summary

smart00220
Location:494 → 746

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14192
Location:486 → 746

STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

86050933..86112969 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006530825.4 → XP_006530888.1 myosin light chain kinase 3 isoform X1

Conserved Domains (1) summary

cd14192
Location:326 → 586

STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3