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    Spag8 sperm associated antigen 8 [ Mus musculus (house mouse) ]

    Gene ID: 433700, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Spag8provided by MGI
    Official Full Name
    sperm associated antigen 8provided by MGI
    Primary source
    MGI:MGI:3056295
    See related
    Ensembl:ENSMUSG00000066196 AllianceGenome:MGI:3056295
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    SMP-1; MH-SPAG8
    Summary
    Predicted to enable microtubule binding activity. Involved in flagellated sperm motility. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in cytoplasm and male germ cell nucleus. Is active in axonemal A tubule inner sheath and sperm flagellum. Orthologous to human SPAG8 (sperm associated antigen 8). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in testis adult (RPKM 31.1), ovary adult (RPKM 6.1) and 4 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Spag8 in Genome Data Viewer
    Location:
    4 A5; 4 23.05 cM
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (43651330..43653594, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (43651330..43653594, complement)

    Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_10057 Neighboring gene predicted gene 12472 Neighboring gene predicted gene, 42279 Neighboring gene natriuretic peptide receptor 2 Neighboring gene histidine triad nucleotide binding protein 2 Neighboring gene small EDRK-rich factor 2 pseudogene Neighboring gene family with sequence similarity 221, member B Neighboring gene small EDRK-rich factor 2 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis. Wu H, et al. FEBS Lett, 2010 Jul 2. PMID 20488182
    2. De novo protein identification in mammalian sperm using in situ cryoelectron tomography and AlphaFold2 docking. Chen Z, et al. Cell, 2023 Nov 9. PMID 37865089, Free PMC Article
    3. In-cell structural insight into the stability of sperm microtubule doublet. Tai L, et al. Cell Discov, 2023 Nov 21. PMID 37989994, Free PMC Article
    4. Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility. Zhou L, et al. Cell, 2023 Jun 22. PMID 37295417
    5. A conditional knockout resource for the genome-wide study of mouse gene function. Skarnes WC, et al. Nature, 2011 Jun 15. PMID 21677750, Free PMC Article

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
      Title: Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 

    General gene information

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    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables microtubule binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell differentiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in flagellated sperm motility IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in single fertilization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in spermatogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in acrosomal vesicle ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in axonemal A tubule inner sheath IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in axonemal microtubule ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in axonemal microtubule ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in male germ cell nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in microtubule organizing center IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in sperm flagellum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in spindle IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    sperm-associated antigen 8
    Names
    sperm associated antigen 8-like protein
    sperm membrane protein 1
    sperm membrane protein BS-84

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001007463.2 → NP_001007464.1  sperm-associated antigen 8 isoform 2

      See identical proteins and their annotated locations for NP_001007464.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) contains a 3' terminal exon that extends past a splice site used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AL732626
      Consensus CDS
      CCDS38743.1
      UniProtKB/Swiss-Prot
      Q3V0Q6
      Related
      ENSMUSP00000103502.3, ENSMUST00000107870.3
      Conserved Domains (1) summary
      PHA03307
      Location:56 → 175
      PHA03307; transcriptional regulator ICP4; Provisional

    2. NM_001290462.2 → NP_001277391.1  sperm-associated antigen 8 isoform 1

      See identical proteins and their annotated locations for NP_001277391.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AK132968, AL732626
      Consensus CDS
      CCDS71376.1
      UniProtKB/Swiss-Prot
      B9EKF1, Q3V0Q6, Q5UAV3
      Related
      ENSMUSP00000081696.5, ENSMUST00000084646.11
      Conserved Domains (1) summary
      PHA03307
      Location:56 → 175
      PHA03307; transcriptional regulator ICP4; Provisional

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000070.7 Reference GRCm39 C57BL/6J

      Range
      43651330..43653594 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q3V0Q6.1 GenPept UniProtKB/Swiss-Prot:Q3V0Q6

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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