Rad9a RAD9 checkpoint clamp component A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rad9aprovided by MGI
Official Full Name: RAD9 checkpoint clamp component Aprovided by MGI
Primary source: MGI:MGI:1328356
See related: Ensembl:ENSMUSG00000024824 AllianceGenome:MGI:1328356
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rad9
Summary: Predicted to enable 3'-5' exonuclease activity; SH3 domain binding activity; and enzyme binding activity. Acts upstream of or within mitotic intra-S DNA damage checkpoint signaling and positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage. Located in cytoplasm and nucleus. Is expressed in male reproductive gland or organ; retina; telencephalon; and thyroid gland. Orthologous to human RAD9A (RAD9 checkpoint clamp component A). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 48.0), spleen adult (RPKM 29.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 A; 19 3.89 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (4245195..4251710, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (4195196..4201672, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RAD9A may also play an important role in maternal chromatin reprogramming. In summary, our data reveal an important role of RAD9A in zygotic chromatin reprogramming and female fertility.
Title: Rad9a is involved in chromatin decondensation and post-zygotic embryo development in mice.
Rad9a is indispensable for spermatogonia differentiation and testicular development in mice.
Title: Rad9a is required for spermatogonia differentiation in mice.
RAD9 has a prominent role in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling.
Title: Regulation of ATRIP protein abundance by RAD9 in the DNA damage repair pathway.
We demonstrated that Mrad9 null enhances chromatid aberration frequency induced by radiation in bystander mouse embryonic stem cells
Title: RAD9 deficiency enhances radiation induced bystander DNA damage and transcriptomal response.
RAD9A is essential for male fertility and for repair of DNA double-strand breaks during meiotic prophase I.
Title: The DNA damage checkpoint protein RAD9A is essential for male meiosis in the mouse.
HUS1 acts as a component of the canonical 9-1-1 complex during meiotic prophase I to promote DSB repair and further propose that RAD1 and TOPBP1 respond to unsynapsed chromatin through an alternative mechanism that does not require RAD9 or HUS1.
Title: Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance.
A review of the many activities assigned to Rad9, and speculation as to which influence its function in tumor development.
Title: Contributions of Rad9 to tumorigenesis.
Data show that Rad9 plays dual roles in generating functional antibodies and in maintaining the integrity of the whole genome in B cells.
Title: Rad9 is required for B cell proliferation and immunoglobulin class switch recombination.
Rad9A-mediated Claspin localization is a vital step during checkpoint activation.
Title: The Rad9A checkpoint protein is required for nuclear localization of the claspin adaptor protein.
tousled-like kinases play important roles in DNA repair, not only by modulation of chromatin assembly via Asf1, but also by a more direct function in processing the ends of a tousled-like kinase via interaction with Rad9.
Title: TLK1B promotes repair of DSBs via its interaction with Rad9 and Asf1.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3'-5' exonuclease activity	ISO Inferred from Sequence Orthology more info
enables SH3 domain binding	IEA Inferred from Electronic Annotation more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables double-stranded DNA 3'-5' DNA exonuclease activity	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	IEA Inferred from Electronic Annotation more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA damage checkpoint signaling	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to ionizing radiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to ionizing radiation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to ionizing radiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of intrinsic apoptotic signaling pathway in response to DNA damage	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of intrinsic apoptotic signaling pathway in response to DNA damage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within mitotic intra-S DNA damage checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to radiation	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Component	Evidence Code	Pubs
part_of checkpoint clamp complex	IBA Inferred from Biological aspect of Ancestor more info
part_of checkpoint clamp complex	IEA Inferred from Electronic Annotation more info
part_of checkpoint clamp complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cell cycle checkpoint control protein RAD9A

Names: DNA repair exonuclease rad9 homolog A; RAD9 homolog A; Rad9-like protein; mRAD9

NP_035367.1

EC 3.1.11.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011237.2 → NP_035367.1 cell cycle checkpoint control protein RAD9A

See identical proteins and their annotated locations for NP_035367.1

Status: PROVISIONAL

Source sequence(s)

AC140073

Consensus CDS

CCDS29422.1

UniProtKB/Swiss-Prot

Q8QZZ3, Q9Z0F6

Related

ENSMUSP00000025740.7, ENSMUST00000025740.8

Conserved Domains (2) summary

cd00577
Location:9 → 267

PCNA; Proliferating Cell Nuclear Antigen (PCNA) domain found in eukaryotes and archaea. These polymerase processivity factors play a role in DNA replication and repair. PCNA encircles duplex DNA in its central cavity, providing a DNA-bound platform for the ...

pfam04139
Location:13 → 265

Rad9; Rad9