Prkg2 protein kinase, cGMP-dependent, type II [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkg2provided by MGI
Official Full Name: protein kinase, cGMP-dependent, type IIprovided by MGI
Primary source: MGI:MGI:108173
See related: Ensembl:ENSMUSG00000029334 AllianceGenome:MGI:108173
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CGKII; Prkgr2; cGK-II
Summary: Predicted to enable identical protein binding activity; mitogen-activated protein kinase binding activity; and protein kinase activity. Involved in cellular hypotonic response and negative regulation of cell volume. Acts upstream of or within circadian regulation of gene expression. Located in plasma membrane. Is expressed in brain; limb segment; lung; and small intestine. Human ortholog(s) of this gene implicated in acromesomelic dysplasia-4 and spondylometaphyseal dysplasia. Orthologous to human PRKG2 (protein kinase cGMP-dependent 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in small intestine adult (RPKM 2.2), frontal lobe adult (RPKM 1.5) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 E3; 5 48.35 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (99077632..99185042, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (98929773..99037375, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

cGMP-dependent kinase 2, Na[+]/H[+] exchanger NHE3, and PDZ-adaptor NHERF2 co-assemble in apical membrane microdomains.
Title: cGMP-dependent kinase 2, Na(+)/H(+) exchanger NHE3, and PDZ-adaptor NHERF2 co-assemble in apical membrane microdomains.
Protein Kinase G2 Is Essential for Skeletal Homeostasis and Adaptation to Mechanical Loading in Male but Not Female Mice.
Title: Protein Kinase G2 Is Essential for Skeletal Homeostasis and Adaptation to Mechanical Loading in Male but Not Female Mice.
Type-2 cGMP-dependent protein kinase suppresses proliferation and carcinogenesis in the colon epithelium.
Title: Type-2 cGMP-dependent protein kinase suppresses proliferation and carcinogenesis in the colon epithelium.
Loss of cGMP-dependent protein kinase II alters ultrasonic vocalizations in mice, a model for speech impairment in human microdeletion 4q21 syndrome.
Title: Loss of cGMP-dependent protein kinase II alters ultrasonic vocalizations in mice, a model for speech impairment in human microdeletion 4q21 syndrome.
Phosphorylation of myelin regulatory factor by PRKG2 mediates demyelination in Huntington's disease.
Title: Phosphorylation of myelin regulatory factor by PRKG2 mediates demyelination in Huntington's disease.
Protein synthesis inhibition promotes nitric oxide generation and activation of CGKII-dependent downstream signaling pathways in the retina.
Title: Protein synthesis inhibition promotes nitric oxide generation and activation of CGKII-dependent downstream signaling pathways in the retina.
Prkg2 determines beta-catenin accumulation that is essential for uterine decidualization in mice.
Title: cGMP-dependent protein kinase II determines β-catenin accumulation that is essential for uterine decidualization in mice.
PKG2 is a key regulator of osteoblast proliferation and post-natal bone formation.
Title: cGMP-dependent protein kinase-2 regulates bone mass and prevents diabetic bone loss.
Study shows that there are brain region-specific effects of cGMP-dependent protein kinase II knock-out on AMPA receptor trafficking that affects animal behavior. Study shows that GluA1 phosphorylation levels differ in various brain regions, and specific behaviors are altered according to region-specific changes in GluA1 phosphorylation.
Title: Brain region-specific effects of cGMP-dependent kinase II knockout on AMPA receptor trafficking and animal behavior.
cGKII may play a role in the integration of information that conveys both anxiety-provoking stimuli as well as the spatial and environmental cues that facilitate functional memory processes and appropriate behavioral response.
Title: cGMP-dependent protein kinase type II knockout mice exhibit working memory impairments, decreased repetitive behavior, and increased anxiety-like traits.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (2) 1 citation

General gene information

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Markers

AW212535 (e-PCR)
- UniSTS:179207

Prkg2 (e-PCR), detects polymorphism
- UniSTS:525464

RH118292 (e-PCR)
- UniSTS:159401

Prkg2 (e-PCR), detects polymorphism
- UniSTS:498353

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cGMP binding	IEA Inferred from Electronic Annotation more info
enables cGMP-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables cGMP-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables mitogen-activated protein kinase binding	ISO Inferred from Sequence Orthology more info
enables mitogen-activated protein kinase binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in cellular hypotonic response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within circadian regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell volume	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of chloride transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chondrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in signal transduction	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cGMP-dependent protein kinase 2

Names: cGK 2; cGK2; cGMP-dependent protein kinase II

NP_032952.3

EC 2.7.11.12

XP_006534883.1

EC 2.7.11.12

XP_006534884.1

EC 2.7.11.12

XP_036020790.1

EC 2.7.11.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008926.4 → NP_032952.3 cGMP-dependent protein kinase 2

See identical proteins and their annotated locations for NP_032952.3

Status: VALIDATED

Source sequence(s)

AC141896, AK038739, AK081422

Consensus CDS

CCDS19459.1

UniProtKB/TrEMBL

Q8C4R2, Q8CAH8

Related

ENSMUSP00000124963.2, ENSMUST00000161490.8

Conserved Domains (4) summary

PTZ00263
Location:417 → 752

PTZ00263; protein kinase A catalytic subunit; Provisional

COG0664
Location:162 → 285

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:286 → 401

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:459 → 718

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

99077632..99185042 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036164897.1 → XP_036020790.1 cGMP-dependent protein kinase 2 isoform X1

UniProtKB/TrEMBL

Q8C4R2

Conserved Domains (2) summary

cd00038
Location:286 → 401

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:459 → 718

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
XM_006534821.3 → XP_006534884.1 cGMP-dependent protein kinase 2 isoform X3

Conserved Domains (1) summary

cd05572
Location:1 → 238

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
XM_006534820.5 → XP_006534883.1 cGMP-dependent protein kinase 2 isoform X2

Conserved Domains (2) summary

cd00038
Location:286 → 401

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

pfam08614
Location:24 → 84

ATG16; Autophagy protein 16 (ATG16)