Trim65 tripartite motif-containing 65 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Trim65provided by MGI
Official Full Name: tripartite motif-containing 65provided by MGI
Primary source: MGI:MGI:2442815
See related: Ensembl:ENSMUSG00000054517 AllianceGenome:MGI:2442815
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4732463G12Rik
Summary: Enables ubiquitin-protein transferase activity. Involved in protein K63-linked ubiquitination. Acts upstream of or within several processes, including innate immune response; positive regulation of protein oligomerization; and positive regulation of type I interferon production. Located in cytoplasm. Orthologous to human TRIM65 (tripartite motif containing 65). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in spleen adult (RPKM 8.3), ovary adult (RPKM 6.0) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 E2; 11 80.91 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (116008015..116022239, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (116124708..116131413, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TRIM65 Suppresses oxLDL-induced Endothelial Inflammation by Interaction with VCAM-1 in Atherogenesis.
Title: TRIM65 Suppresses oxLDL-induced Endothelial Inflammation by Interaction with VCAM-1 in Atherogenesis.
TRIM65 knockout inhibits the development of HCC by polarization tumor-associated macrophages towards M1 phenotype via JAK1/STAT1 signaling pathway.
Title: TRIM65 knockout inhibits the development of HCC by polarization tumor-associated macrophages towards M1 phenotype via JAK1/STAT1 signaling pathway.
E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury through inhibition of TOX4-mediated apoptosis.
Title: E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury through inhibition of TOX4-mediated apoptosis.
Trim65 attenuates isoproterenol-induced cardiac hypertrophy by promoting autophagy and ameliorating mitochondrial dysfunction via the Jak1/Stat1 signaling pathway.
Title: Trim65 attenuates isoproterenol-induced cardiac hypertrophy by promoting autophagy and ameliorating mitochondrial dysfunction via the Jak1/Stat1 signaling pathway.
The E3 Ubiquitin Ligase TRIM65 Negatively Regulates Inflammasome Activation Through Promoting Ubiquitination of NLRP3.
Title: The E3 Ubiquitin Ligase TRIM65 Negatively Regulates Inflammasome Activation Through Promoting Ubiquitination of NLRP3.
TRIM65 as an essential component for the MDA5 signaling pathway and provide physiological evidence showing that ubiquitination is important for MDA5 oligomerization and activation.
Title: TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunity.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (7) 1 citation
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info	PubMed
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within antiviral innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of autophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of autophagy	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of interferon-alpha production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of interferon-beta production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interferon-beta production	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of protein oligomerization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein K48-linked ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within type I interferon-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM65

Names: tripartite motif-containing protein 65

NP_848917.2

EC 2.3.2.27

XP_017170108.1

EC 2.3.2.27

XP_036012674.1

EC 2.3.2.27

XP_036012675.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_178802.4 → NP_848917.2 E3 ubiquitin-protein ligase TRIM65

See identical proteins and their annotated locations for NP_848917.2

Status: VALIDATED

Source sequence(s)

AK028857, AK050080, AK079982, BE850668

Consensus CDS

CCDS48981.1

UniProtKB/Swiss-Prot

A2A867, Q6PGD3, Q8BFW4

UniProtKB/TrEMBL

A4FUV9

Related

ENSMUSP00000063410.4, ENSMUST00000067632.4

Conserved Domains (4) summary

cd00021
Location:96 → 132

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:12 → 55

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cd12896
Location:325 → 504

SPRY_PRY_TRIM65; PRY/SPRY domain in tripartite motif-containing domain 65 (TRIM65)

cl21478
Location:129 → 228

ATP-synt_B; ATP synthase B/B' CF(0)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

116008015..116022239 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156781.1 → XP_036012674.1 E3 ubiquitin-protein ligase TRIM65 isoform X1

Conserved Domains (5) summary

COG0711
Location:135 → 228

AtpF; FoF1-type ATP synthase, membrane subunit b or b' [Energy production and conversion]

cd00162
Location:13 → 51

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cl00034
Location:95 → 136

Bbox_SF; B-box-type zinc finger superfamily

cl02614
Location:325 → 394

SPRY; SPRY domain

cl17238
Location:11 → 52

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
XM_036156782.1 → XP_036012675.1 E3 ubiquitin-protein ligase TRIM65 isoform X2

Related

ENSMUSP00000102048.3, ENSMUST00000106440.9

Conserved Domains (5) summary

COG0711
Location:135 → 228

AtpF; FoF1-type ATP synthase, membrane subunit b or b' [Energy production and conversion]

cd00162
Location:13 → 51

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cl00034
Location:95 → 136

Bbox_SF; B-box-type zinc finger superfamily

cl02614
Location:325 → 393

SPRY; SPRY domain

cl17238
Location:11 → 52

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
XM_017314619.3 → XP_017170108.1 E3 ubiquitin-protein ligase TRIM65 isoform X3

Conserved Domains (4) summary

cd00162
Location:13 → 51

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

pfam12128
Location:140 → 243

DUF3584; Protein of unknown function (DUF3584)

cl00034
Location:95 → 136

Bbox_SF; B-box-type zinc finger superfamily

cl17238
Location:11 → 52

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain