Pdcl phosducin-like [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pdclprovided by MGI
Official Full Name: phosducin-likeprovided by MGI
Primary source: MGI:MGI:1914716
See related: Ensembl:ENSMUSG00000009030 AllianceGenome:MGI:1914716
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PhLP1; 1200011E13Rik
Summary: Involved in positive regulation of smoothened signaling pathway. Acts upstream of or within heterotrimeric G-protein complex assembly and visual perception. Predicted to be located in cilium. Predicted to be active in cytoplasm. Orthologous to human PDCL (phosducin like). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 13.6), CNS E14 (RPKM 11.9) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 B; 2 24.19 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (37240086..37249832, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (37350074..37359820, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pdcl has been implicated as a positive regulator of Hedgehog signaling that may control the trafficking of signaling receptors to primary cilia.
Title: CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.
findings reveal a common mechanism of Gbetagamma and RGS9-Gbeta5 assembly in rods and cones, highlighting the importance of PhLP1 and CCT-mediated Gbeta complex formation in G protein signaling.
Title: Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling.
evidence of a generic mechanism, whereby the splicing of the PhLP gene could potentially and efficiently regulate the cellular levels of heterotrimeric G proteins.
Title: Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.
this study demonstrated in vivo that PhLP1 is required for the folding and assembly of both Gbetagamma and Gbeta5-RGS9.
Title: Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.
These data are consistent with the hypothesis that PhLP is a widely expressed modulator of Gbetagamma function.
Title: Interaction of phosducin-like protein with G protein betagamma subunits.
These results suggest a mechanism for Gbetagamma assembly in which PhLP stabilizes the nascent Gbeta polypeptide until Ggamma can associate, resulting in membrane binding of Gbetagamma and release of PhLP to catalyze another round of assembly.
Title: Phosducin-like protein acts as a molecular chaperone for G protein betagamma dimer assembly.
N-glycosylated phosducin-like protein long (PhLP(L)) is expressed in all structures of the central nervous system and regulates opioid receptor function in the brain.
Title: Glycosylated phosducin-like protein long regulates opioid receptor function in mouse brain.
physiological control of G-protein regulation by PhLP seems to involve phosphorylation by CK2 and alternative splicing of the regulator
Title: Regulation of phosducin-like protein by casein kinase 2 and N-terminal splicing.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:234380

AW108059 (e-PCR)
- UniSTS:178613

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell projection organization	IEA Inferred from Electronic Annotation more info
involved_in heterotrimeric G-protein complex assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within heterotrimeric G-protein complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein refolding	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein folding	ISO Inferred from Sequence Orthology more info
involved_in regulation of G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within visual perception	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cilium	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: phosducin-like protein

Names: PHLP

NP_080452.2

EC 1.14.99.1

XP_006498315.1

EC 1.14.99.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_026176.3 → NP_080452.2 phosducin-like protein

Status: VALIDATED

Source sequence(s)

AK028668, AV286628, BY024444

Consensus CDS

CCDS15998.1

UniProtKB/Swiss-Prot

Q3TKI0, Q9DBX2

UniProtKB/TrEMBL

Q3UZN6

Related

ENSMUSP00000009174.9, ENSMUST00000009174.15

Conserved Domains (1) summary

pfam02114
Location:35 → 299

Phosducin

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

37240086..37249832 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006498252.1 → XP_006498315.1 phosducin-like protein isoform X1

UniProtKB/Swiss-Prot

Q3TKI0, Q9DBX2

UniProtKB/TrEMBL

Q3UZN6

Conserved Domains (1) summary

pfam02114
Location:35 → 299

Phosducin