Phlpp2 PH domain and leucine rich repeat protein phosphatase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Phlpp2provided by MGI
Official Full Name: PH domain and leucine rich repeat protein phosphatase 2provided by MGI
Primary source: MGI:MGI:2444928
See related: Ensembl:ENSMUSG00000031732 AllianceGenome:MGI:2444928
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Phlppl; C130044A18Rik
Summary: Predicted to enable protein serine/threonine phosphatase activity. Predicted to be involved in intracellular signal transduction. Predicted to be located in several cellular components, including mitotic spindle; photoreceptor inner segment; and photoreceptor outer segment membrane. Predicted to be active in cytoplasm. Is expressed in cerebellum; hippocampus; liver; and retina outer nuclear layer. Orthologous to human PHLPP2 (PH domain and leucine rich repeat protein phosphatase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in large intestine adult (RPKM 7.7), small intestine adult (RPKM 6.5) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 D3; 8 57.33 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (110595149..110671303)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (109868517..109944671)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Knockdown of Mmu-circ-0001380 Attenuates Myocardial Ischemia/Reperfusion Injury via Modulating miR-106b-5p/Phlpp2 Axis.
Title: Knockdown of Mmu-circ-0001380 Attenuates Myocardial Ischemia/Reperfusion Injury via Modulating miR-106b-5p/Phlpp2 Axis.
PHLPP isoforms differentially regulate Akt isoforms and AS160 affecting neuronal insulin signaling and insulin resistance via Scribble.
Title: PHLPP isoforms differentially regulate Akt isoforms and AS160 affecting neuronal insulin signaling and insulin resistance via Scribble.
Inhibition of PHLPP1/2 phosphatases rescues pancreatic beta-cells in diabetes.
Title: Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes.
Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-kappaB Signaling Pathway.
Title: Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway.
Adipocyte PHLPP2 inhibition prevents obesity-induced fatty liver.
Title: Adipocyte PHLPP2 inhibition prevents obesity-induced fatty liver.
Endothelial Scaffolding Protein ENH (Enigma Homolog Protein) Promotes PHLPP2 (Pleckstrin Homology Domain and Leucine-Rich Repeat Protein Phosphatase 2)-Mediated Dephosphorylation of AKT1 and eNOS (Endothelial NO Synthase) Promoting Vascular Remodeling.
Title: Endothelial Scaffolding Protein ENH (Enigma Homolog Protein) Promotes PHLPP2 (Pleckstrin Homology Domain and Leucine-Rich Repeat Protein Phosphatase 2)-Mediated Dephosphorylation of AKT1 and eNOS (Endothelial NO Synthase) Promoting Vascular Remodeling.
PHLPP2 was identified as a direct target of miR-130a-3p, and the hepatic exosome-derived miR-130a-3p could improve glucose intolerance via suppressing PHLPP2 to activate AKT-AS160-GLUT4 signaling pathway in adipocytes.
Title: Hepatic exosome-derived miR-130a-3p attenuates glucose intolerance via suppressing PHLPP2 gene in adipocyte.
PHLPP2 silencing increased the phosphorylation of glycogen synthase kinase (GSK)-3beta and promoted the nuclear translocation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2). In addition, PHLPP2 inhibition promoted Nrf2/antioxidant response element (ARE) transcriptional activity.
Title: PHLPP2 downregulation protects cardiomyocytes against hypoxia-induced injury through reinforcing Nrf2/ARE antioxidant signaling.
KCTD17, which is up-regulated in liver tissues of obese mice and patients with NAFLD, binds to phosphorylated PHLPP2 to target it for ubiquitin-mediated degradation; this increases expression of genes that regulate lipogenesis to promote hepatic steatosis.
Title: Degradation of PHLPP2 by KCTD17, via a Glucagon-Dependent Pathway, Promotes Hepatic Steatosis.
results identify a novel role of PHLPP in regulating aPKC and cell polarity.
Title: Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI481772 (e-PCR)
- UniSTS:159814

D8Mit269 (e-PCR), detects polymorphism
- UniSTS:131479

D14Sfk4 (e-PCR), detects polymorphism
- UniSTS:141994

NoName (e-PCR)
- UniSTS:225278

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables MAP kinase serine/threonine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables calmodulin-dependent protein phosphatase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS140 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine phosphatase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in photoreceptor inner segment	ISO Inferred from Sequence Orthology more info
located_in photoreceptor outer segment membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: PH domain leucine-rich repeat-containing protein phosphatase 2

Names: GTPase activating protein and VPS9 domains 1

NP_001116066.2

EC 3.1.3.16

XP_006531073.1

EC 3.1.3.16

XP_030099411.1

EC 3.1.3.16

XP_036009935.1

EC 3.1.3.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001122594.2 → NP_001116066.2 PH domain leucine-rich repeat-containing protein phosphatase 2

See identical proteins and their annotated locations for NP_001116066.2

Status: VALIDATED

Source sequence(s)

AC122807, AI481772, AK048472

Consensus CDS

CCDS57644.1

UniProtKB/Swiss-Prot

Q148U6, Q8BX96, Q8BXA7

UniProtKB/TrEMBL

J3QM82

Related

ENSMUSP00000136166.2, ENSMUST00000179721.8

Conserved Domains (5) summary

cd00116
Location:447 → 714

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

cd00143
Location:816 → 1065

PP2Cc; Serine/threonine phosphatases, family 2C, catalytic domain; The protein architecture and deduced catalytic mechanism of PP2C phosphatases are similar to the PP1, PP2A, PP2B family of protein Ser/Thr phosphatases, with which PP2C shares no sequence ...

cd13322
Location:181 → 275

PH_PHLPP-like; PH domain leucine-rich repeat protein phosphatase family Pleckstrin homology-like domain

sd00033
Location:514 → 535

LRR_RI; leucine-rich repeat [structural motif]

pfam13855
Location:676 → 735

LRR_8; Leucine rich repeat

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

110595149..110671303

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006531010.4 → XP_006531073.1 PH domain leucine-rich repeat-containing protein phosphatase 2 isoform X2

Conserved Domains (4) summary

PLN00113
Location:280 → 758

PLN00113; leucine-rich repeat receptor-like protein kinase; Provisional

cd13322
Location:181 → 275

PH_PHLPP-like; PH domain leucine-rich repeat protein phosphatase family Pleckstrin homology-like domain

sd00033
Location:514 → 535

LRR_RI; leucine-rich repeat [structural motif]

cd17241
Location:66 → 174

RA_PHLPP2; Ras-associating (RA) domain found in PH domain leucine-rich repeat-containing protein phosphatase 2 (PHLPP2)
XM_036154042.1 → XP_036009935.1 PH domain leucine-rich repeat-containing protein phosphatase 2 isoform X1

UniProtKB/Swiss-Prot

Q148U6, Q8BX96, Q8BXA7

Conserved Domains (4) summary

PLN00113
Location:114 → 592

PLN00113; leucine-rich repeat receptor-like protein kinase; Provisional

cd00143
Location:650 → 899

PP2Cc; Serine/threonine phosphatases, family 2C, catalytic domain; The protein architecture and deduced catalytic mechanism of PP2C phosphatases are similar to the PP1, PP2A, PP2B family of protein Ser/Thr phosphatases, with which PP2C shares no sequence ...

cd13322
Location:15 → 109

PH_PHLPP-like; PH domain leucine-rich repeat protein phosphatase family Pleckstrin homology-like domain

sd00033
Location:348 → 369

LRR_RI; leucine-rich repeat [structural motif]
XM_030243551.1 → XP_030099411.1 PH domain leucine-rich repeat-containing protein phosphatase 2 isoform X3

Conserved Domains (3) summary

PRK15370
Location:12 → 208

PRK15370; type III secretion system effector E3 ubiquitin transferase SlrP

cd00143
Location:243 → 492

PP2Cc; Serine/threonine phosphatases, family 2C, catalytic domain; The protein architecture and deduced catalytic mechanism of PP2C phosphatases are similar to the PP1, PP2A, PP2B family of protein Ser/Thr phosphatases, with which PP2C shares no sequence ...

sd00033
Location:9 → 29

LRR_RI; leucine-rich repeat [structural motif]