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    AS2 Lateral organ boundaries (LOB) domain family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 842873, updated on 18-Sep-2024

    Summary

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    Official Symbol
    AS2
    Official Full Name
    Lateral organ boundaries (LOB) domain family protein
    Primary source
    TAIR:AT1G65620
    Locus tag
    AT1G65620
    See related
    Araport:AT1G65620
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ASYMMETRIC LEAVES 2; F5I14.15; F5I14_15
    Summary
    required for formation of a symmetric flat leaf lamina, encodes a member of a family of proteins characterized by cysteine repeats and a leucine zipper; involved in KNOX gene regulation. Acts together with ASL1 in proximal-distal symmetry determination. Forms a complex with AS1 that binds to the BP promoter and leads to silencing of BP.
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    Genomic context

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    See AS2 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    4
    Sequence:
    Chromosome: 1; NC_003070.9 (24398143..24400989)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene beta-hexosaminidase 3 Neighboring gene Six-hairpin glycosidases superfamily protein Neighboring gene DegP protease 3 Neighboring gene DegP protease 4

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves. Semiarti E, et al. Development, 2001 May. PMID 11311158
    2. The formation of perinucleolar bodies is important for normal leaf development and requires the zinc-finger DNA-binding motif in Arabidopsis ASYMMETRIC LEAVES2. Luo L, et al. Plant J, 2020 Mar. PMID 31639235, Free PMC Article
    3. Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves. Iwakawa H, et al. Plant J, 2007 Jul. PMID 17559509
    4. Quantitative control of ASYMMETRIC LEAVES2 expression is critical for leaf axial patterning in Arabidopsis. Chen X, et al. J Exp Bot, 2013 Nov. PMID 24006428, Free PMC Article
    5. ASYMMETRIC LEAVES2 gene, a member of LOB/AS2 family of Arabidopsis thaliana, causes an abaxializing leaves in transgenic cockscomb. Sun SB, et al. Mol Biol Rep, 2012 Apr. PMID 22143880

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Roles of ASYMMETRIC LEAVES2 (AS2) and Nucleolar Proteins in the Adaxial-Abaxial Polarity Specification at the Perinucleolar Region in Arabidopsis.
      Title: Roles of ASYMMETRIC LEAVES2 (AS2) and Nucleolar Proteins in the Adaxial-Abaxial Polarity Specification at the Perinucleolar Region in Arabidopsis.
    2. The formation of perinucleolar bodies is important for normal leaf development and requires the zinc-finger DNA-binding motif in Arabidopsis ASYMMETRIC LEAVES2.
      Title: The formation of perinucleolar bodies is important for normal leaf development and requires the zinc-finger DNA-binding motif in Arabidopsis ASYMMETRIC LEAVES2.
    3. AS2 role in the DNA methylation and leaf polarity.AS2 binds specifically the sequence containing CpGs in exon 1 of ARF3.
      Title: Arabidopsis Zinc-Finger-Like Protein ASYMMETRIC LEAVES2 (AS2) and Two Nucleolar Proteins Maintain Gene Body DNA Methylation in the Leaf Polarity Gene ETTIN (ARF3).
    4. that complexes of the transcription factors ASYMMETRIC LEAVES 1 (AS1) and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS and KNAT2 via direct interactions with LHP1.
      Title: Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis.
    5. These data points to a general requirement for AS2 repression in meristematic regions to allow cell proliferation.
      Title: Repression of AS2 by WOX family transcription factors is required for leaf development in Medicago and Arabidopsis.
    6. We found that AS2 promotes DCP2 decapping activity, accelerates mRNA turnover rate, inhibits siRNA accumulation and functions as an endogenous suppressor of post-transcriptional gene silencing
      Title: Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis.
    7. ASYMMETRIC LEAVES2 expression is critical for leaf axial patterning.
      Title: Quantitative control of ASYMMETRIC LEAVES2 expression is critical for leaf axial patterning in Arabidopsis.
    8. Negative transcriptional, post-transcriptional and epigenetic regulation of ARF3 by AS1-AS2 is important for stabilizing early leaf partitioning into abaxial and adaxial domains.
      Title: Dual regulation of ETTIN (ARF3) gene expression by AS1-AS2, which maintains the DNA methylation level, is involved in stabilization of leaf adaxial-abaxial partitioning in Arabidopsis.
    9. The JLO and AS2 proteins interact molecularly and form multimeric complexes with AS1 to suppress KNOX expression. AS2 together with JLO regulate auxin transport in seedling roots.
      Title: Arabidopsis JAGGED LATERAL ORGANS acts with ASYMMETRIC LEAVES2 to coordinate KNOX and PIN expression in shoot and root meristems.
    10. These results suggest that AS2 bodies function to properly distribute AS2 to daughter cells during cell division in leaf primordia; and this process is controlled at least partially by signals encoded by the AS2 sequence itself.
      Title: Arabidopsis ASYMMETRIC LEAVES2 protein required for leaf morphogenesis consistently forms speckles during mitosis of tobacco BY-2 cells via signals in its specific sequence.
    Submit: New GeneRIF Correction See all GeneRIFs (23)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001084308.5
    NM_001084309.2
    NM_001124081.2
    NM_001334217.1
    NM_105235.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables DNA-binding transcription activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within adaxial/abaxial axis specification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within petal development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within polarity specification of adaxial/abaxial axis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within proximal/distal pattern formation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within specification of symmetry IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    Lateral organ boundaries (LOB) domain family protein
    NP_001077777.1
    • ASYMMETRIC LEAVES 2 (AS2); FUNCTIONS IN: protein binding; INVOLVED IN: in 6 processes; LOCATED IN: nucleoplasm, nucleus; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Lateral organ boundaries, LOB (InterPro:IPR004883); BEST Arabidopsis thaliana protein match is: ASYMMETRIC LEAVES 2-like 1 (TAIR:AT5G66870.1); Has 984 Blast hits to 979 proteins in 24 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 984; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
    NP_001077778.1
    • ASYMMETRIC LEAVES 2 (AS2); FUNCTIONS IN: protein binding; INVOLVED IN: in 6 processes; LOCATED IN: nucleoplasm, nucleus; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Lateral organ boundaries, LOB (InterPro:IPR004883); BEST Arabidopsis thaliana protein match is: ASYMMETRIC LEAVES 2-like 1 (TAIR:AT5G66870.1); Has 984 Blast hits to 979 proteins in 24 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 984; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
    NP_001117553.1
    • ASYMMETRIC LEAVES 2 (AS2); FUNCTIONS IN: protein binding; INVOLVED IN: in 6 processes; LOCATED IN: nucleoplasm, nucleus; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Lateral organ boundaries, LOB (InterPro:IPR004883); BEST Arabidopsis thaliana protein match is: ASYMMETRIC LEAVES 2-like 1 (TAIR:AT5G66870.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    NP_176739.1
    • ASYMMETRIC LEAVES 2 (AS2); CONTAINS InterPro DOMAIN/s: Lateral organ boundaries, LOB (InterPro:IPR004883); BEST Arabidopsis thaliana protein match is: ASYMMETRIC LEAVES 2-like 1 (TAIR:AT5G66870.1); Has 984 Blast hits to 979 proteins in 24 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 984; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      24398143..24400989
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001084308.5 → NP_001077777.1  Lateral organ boundaries (LOB) domain family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001077777.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B7XG54, O04479, Q53YQ2
      UniProtKB/TrEMBL
      A0A178WIA0, A0A5S9WS75
      Conserved Domains (1) summary
      pfam03195
      Location:9 → 89
      LOB; Lateral organ boundaries (LOB) domain

    2. NM_105235.5 → NP_176739.1  Lateral organ boundaries (LOB) domain family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_176739.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B7XG54, O04479, Q53YQ2
      UniProtKB/TrEMBL
      A0A178WIA0, A0A5S9WS75
      Conserved Domains (1) summary
      pfam03195
      Location:9 → 89
      LOB; Lateral organ boundaries (LOB) domain

    3. NM_001084309.2 → NP_001077778.1  Lateral organ boundaries (LOB) domain family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001077778.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B7XG54, O04479, Q53YQ2
      UniProtKB/TrEMBL
      A0A178WIA0, A0A5S9WS75
      Conserved Domains (1) summary
      pfam03195
      Location:9 → 89
      LOB; Lateral organ boundaries (LOB) domain

    4. NM_001124081.2 → NP_001117553.1  Lateral organ boundaries (LOB) domain family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001117553.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B7XG54, O04479, Q53YQ2
      UniProtKB/TrEMBL
      A0A178WIA0, A0A5S9WS75
      Conserved Domains (1) summary
      pfam03195
      Location:9 → 89
      LOB; Lateral organ boundaries (LOB) domain

    5. NM_001334217.1 → NP_001320370.1  Lateral organ boundaries (LOB) domain family protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B7XG54, O04479, Q53YQ2
      UniProtKB/TrEMBL
      A0A178WIA0, A0A5S9WS75
      Conserved Domains (1) summary
      pfam03195
      Location:9 → 89
      LOB; Lateral organ boundaries (LOB) domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O04479.1

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