Aqp8 aquaporin 8 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Aqp8provided by RGD
Official Full Name: aquaporin 8provided by RGD
Primary source: RGD:2146
See related: EnsemblRapid:ENSRNOG00000014652 AllianceGenome:RGD:2146
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: AQP-8
Summary: Enables ammonium channel activity; methylammonium channel activity; and water channel activity. Involved in several processes, including ammonium import across plasma membrane; canalicular bile acid transport; and hydrogen peroxide transmembrane transport. Located in several cellular components, including basolateral plasma membrane; brush border membrane; and intracellular canaliculus. Biomarker of brain ischemia. Orthologous to human AQP8 (aquaporin 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Liver (RPKM 791.1), Testes (RPKM 189.1) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 1q36

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (187424314..187430243)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (177993313..177999158)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (193424818..193430665)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AQP8 is a crucial H2O2 transporter in insulin-producing RINm5F cells.
Title: AQP8 is a crucial H(2)O(2) transporter in insulin-producing RINm5F cells.
Aquaporin 8 is involved in H2 O2 -mediated differential regulation of metabolic signaling by alpha1 - and beta-adrenoceptors in hepatocytes.
Title: Aquaporin 8 is involved in H(2) O(2) -mediated differential regulation of metabolic signaling by α(1) - and β-adrenoceptors in hepatocytes.
Mitochondrial AQP8 is involved in SREBP2-controlled hepatocyte cholesterol biosynthesis.
Title: Mitochondrial aquaporin-8 is involved in SREBP-controlled hepatocyte cholesterol biosynthesis.
liquid water metabolic abnormalities and intestine permeability might be the mechanism of IBS by regulating AQP1, AQP3 and AQP8 via NF-kappaB pathway.
Title: Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats' colon via NF-κB pathway.
in vitro and in vivo data suggest that hepatic mtAQP8 expression is downregulated in acidosis, a mechanism that may contribute to decreased ureagenesis from ammonia in response to acidosis
Title: Acidosis-induced downregulation of hepatocyte mitochondrial aquaporin-8 and ureagenesis from ammonia.
Downregulation of hepatocyte mtAQP8 expression leads to defective ureagenesis from ammonia in lipopolysaccharide-treated rats.
Title: Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: involvement of mitochondrial aquaporin-8.
The results obtained here implied that both AQP3 and AQP8 may be involved in the pathogenesis of inflammatory bowel disease.
Title: Aquaporin 3 and 8 are down-regulated in TNBS-induced rat colitis.
Hepatocyte mtAQP8 channels facilitate the mitochondrial uptake of ammonia and its metabolism into urea, mainly under glucagon stimulation.
Title: Ammonia detoxification via ureagenesis in rat hepatocytes involves mitochondrial aquaporin-8 channels.
There are no significant changes in the expressions of aquaporin 4 and 8 in rat models with slow transit constipation.
Title: [Expression of aquaporin 3, 4, and 8 in colonic mucosa of rat models with slow transit constipation].
AQP8 plays an important role in the adaptive response of proximal tubule to acidosis possibly facilitating mitochondrial ammonia transport
Title: Mitochondrial aquaporin-8 in renal proximal tubule cells: evidence for a role in the response to metabolic acidosis.

Submit: New GeneRIF Correction See all GeneRIFs (28)

General gene information

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Markers

RH142181 (e-PCR)
- UniSTS:231431

RH136820 (e-PCR)
- UniSTS:218909

Aqp8 (e-PCR), detects polymorphism
- UniSTS:186497

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ammonium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables ammonium channel activity	IEA Inferred from Electronic Annotation more info
enables ammonium channel activity	ISO Inferred from Sequence Orthology more info
enables ammonium channel activity	ISS Inferred from Sequence or Structural Similarity more info
enables channel activity	IEA Inferred from Electronic Annotation more info
enables methylammonium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables methylammonium channel activity	IEA Inferred from Electronic Annotation more info
enables methylammonium channel activity	ISO Inferred from Sequence Orthology more info
enables urea channel activity	IEA Inferred from Electronic Annotation more info
enables urea channel activity	ISO Inferred from Sequence Orthology more info
enables urea channel activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables water channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables water channel activity	IDA Inferred from Direct Assay more info	PubMed
enables water channel activity	IEA Inferred from Electronic Annotation more info
enables water channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables water channel activity	ISO Inferred from Sequence Orthology more info
enables water channel activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in B cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in B cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in B cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in ammonium import across plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in ammonium import across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in ammonium import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in ammonium transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in ammonium transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in canalicular bile acid transport	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular detoxification	IEA Inferred from Electronic Annotation more info
involved_in cellular detoxification	ISO Inferred from Sequence Orthology more info
involved_in cellular detoxification	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to cAMP	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cAMP	ISO Inferred from Sequence Orthology more info
involved_in hydrogen peroxide transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in hydrogen peroxide transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hydrogen peroxide transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in hydrogen peroxide transmembrane transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in methylammonium transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in methylammonium transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in methylammonium transport	IDA Inferred from Direct Assay more info	PubMed
involved_in methylammonium transport	ISO Inferred from Sequence Orthology more info
involved_in regulation of cholesterol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of cholesterol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of cholesterol biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in transepithelial water transport	IDA Inferred from Direct Assay more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in urea transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in urea transport	ISO Inferred from Sequence Orthology more info
involved_in urea transport	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in water transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in water transport	IDA Inferred from Direct Assay more info	PubMed
involved_in water transport	IEA Inferred from Electronic Annotation more info
involved_in water transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within water transport	ISO Inferred from Sequence Orthology more info
involved_in water transport	ISO Inferred from Sequence Orthology more info
involved_in water transport	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endomembrane system	IEA Inferred from Electronic Annotation more info
located_in intracellular canaliculus	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular canaliculus	IEA Inferred from Electronic Annotation more info
located_in intracellular canaliculus	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in intracellular vesicle	IEA Inferred from Electronic Annotation more info
located_in intracellular vesicle	ISO Inferred from Sequence Orthology more info
located_in intracellular vesicle	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in smooth endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in smooth endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in smooth endoplasmic reticulum	ISS Inferred from Sequence or Structural Similarity more info
located_in smooth endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: aquaporin-8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019158.2 → NP_062031.1 aquaporin-8

See identical proteins and their annotated locations for NP_062031.1

Status: PROVISIONAL

Source sequence(s)

BC081812

UniProtKB/Swiss-Prot

P56405

UniProtKB/TrEMBL

A0A8I6GK50

Conserved Domains (1) summary

cl00200
Location:40 → 250

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

187424314..187430243

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006230187.5 → XP_006230249.1 aquaporin-8 isoform X1

See identical proteins and their annotated locations for XP_006230249.1

UniProtKB/TrEMBL

A0A0H2UHM1, A0A8I6GK50, A6I904

Related

ENSRNOP00000019939.6, ENSRNOT00000019939.8

Conserved Domains (1) summary

cl00200
Location:39 → 249

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...