N6amt1 N-6 adenine-specific DNA methyltransferase 1 (putative) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: N6amt1provided by MGI
Official Full Name: N-6 adenine-specific DNA methyltransferase 1 (putative)provided by MGI
Primary source: MGI:MGI:1915018
See related: Ensembl:ENSMUSG00000044442 AllianceGenome:MGI:1915018
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Hemk2; Pred28; 5830445C04Rik
Summary: Enables protein-glutamine N-methyltransferase activity. Involved in peptidyl-glutamine methylation. Located in nucleus. Is expressed in several structures, including genitourinary system; gut; nervous system; sensory organ; and thymus. Orthologous to human N6AMT1 (N-6 adenine-specific DNA methyltransferase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in bladder adult (RPKM 6.9), ovary adult (RPKM 5.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 C3.3; 16 49.52 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (87151073..87165537)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (87354185..87368649)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DNA N6-methyladenine methylase N6AMT1 controls neuropathic pain through epigenetically modifying Kcnj16 in dorsal horn neurons.
Title: DNA N6-methyladenine methylase N6AMT1 controls neuropathic pain through epigenetically modifying Kcnj16 in dorsal horn neurons.
HEMK2 requires a GQX3R motif for methylation activity.
Title: Substrate Specificity of the HEMK2 Protein Glutamine Methyltransferase and Identification of Novel Substrates.
Disruption of N6amt1 gene led to early embryonic lethality.
Title: Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality.
The murine PRED28 encodes two alternatively spliced RNA.
Title: Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.
suggested that mN6A1 participates in protein translation through interaction with eRF1
Title: RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

N6amt1-pending (e-PCR), detects polymorphism
- UniSTS:259110

AU048745 (e-PCR)
- UniSTS:145195

N6amt1-pending (e-PCR), detects polymorphism
- UniSTS:259637

NoName (e-PCR)
- UniSTS:493194

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables S-adenosyl-L-methionine binding	ISO Inferred from Sequence Orthology more info
enables S-adenosyl-L-methionine binding	ISS Inferred from Sequence or Structural Similarity more info
enables S-adenosylmethionine-dependent methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arsenite methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables arsenite methyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables histone H4K12 methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables histone H4K12 methyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein methyltransferase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables protein-glutamine N-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-glutamine N-methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables site-specific DNA-methyltransferase (adenine-specific) activity	ISO Inferred from Sequence Orthology more info
NOT enables site-specific DNA-methyltransferase (adenine-specific) activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in arsonoacetate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in arsonoacetate metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in methylation	ISO Inferred from Sequence Orthology more info
involved_in methylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of gene expression, epigenetic	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of gene expression, epigenetic	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-glutamine methylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-glutamine methylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell growth	ISO Inferred from Sequence Orthology more info	PubMed
involved_in toxin metabolic process	ISO Inferred from Sequence Orthology more info
involved_in toxin metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in transcription initiation-coupled chromatin remodeling	ISO Inferred from Sequence Orthology more info
involved_in transcription initiation-coupled chromatin remodeling	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of eRF1 methyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: methyltransferase N6AMT1

Names: HemK methyltransferase family member 2; M-N6DNA MTase A; N(6)-adenine-specific DNA methyltransferase 1; N6-DNA methyltransferase A; lysine N-methyltransferase 9; methylarsonite methyltransferase N6AMT1; protein N(5)-glutamine methyltransferase

NP_001152803.1

EC 2.1.1.72

NP_080642.1

EC 2.1.1.72

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001159331.1 → NP_001152803.1 methyltransferase N6AMT1 isoform 2

See identical proteins and their annotated locations for NP_001152803.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AC140319, AY456393

Consensus CDS

CCDS49889.1

UniProtKB/TrEMBL

Q6PRU9, Q6SKR2

Related

ENSMUSP00000113229.2, ENSMUST00000118310.8

Conserved Domains (1) summary

cl17173
Location:21 → 133

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
NM_026366.2 → NP_080642.1 methyltransferase N6AMT1 isoform 1

See identical proteins and their annotated locations for NP_080642.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC140319, AY456393

Consensus CDS

CCDS28289.1

UniProtKB/Swiss-Prot

D3Z6J0, E9PXT7, Q4KMV5, Q6PRU9, Q6SKR2

UniProtKB/TrEMBL

A0A4W7N3B2

Related

ENSMUSP00000061835.5, ENSMUST00000054442.11

Conserved Domains (1) summary

cl17173
Location:21 → 213

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...